搜风祛痰中药复方对AopE基因敲除小鼠动脉粥样硬化易损斑块Beclin-1和Bcl-2基因表达的影响

发布时间：2018-06-15 12:35

本文选题：痰风理论 + ApoE基因敲除小鼠　；参考：《中华中医药学刊》2015年09期

【摘要】：目的:观察搜风祛痰中药复方稳斑汤对ApoE基因敲除小鼠动脉粥样硬化易损斑块Beclin-1和Bcl-2基因表达的影响。方法:建立ApoE基因敲除小鼠动脉粥样硬化不稳定斑块模型,并设立空白组、模型组、西药组、稳斑汤低、中、高剂量组。13周后将小鼠麻醉处死,取经HE染色的主动脉组织在光学显微镜下进行病理学观察,并采用半定量RT-PCR技术检测Beclin-1和Bcl-2基因表达的变化。结果:与空白组相比,模型组Beclin-1mRNA表达水平明显下降(P0.01);与模型组相比,西药组和稳斑汤各剂量组均能提高Beclin-1mRNA表达水平(P0.01);与西药组相比,中药高剂量组Beclin1mRNA表达水平无明显差异(P0.05)。与空白组对比,模型组Bcl-2mRNA表达水平显著降低(P0.05);与模型组相比,西药组和稳斑汤各剂量组均能提高Bcl-2mRNA表达水平(P0.01);与西药组相比,稳斑汤高剂量组Bcl-2mRNA表达水平无明显差异(P0.05)。光镜下观察西药组和稳斑汤各剂量组炎症反应均明显弱于模型组。结论:搜风祛痰中药复方稳斑汤可能通过上调ApoE基因敲除小鼠AS易损斑块中Beclin-1和Bcl-2的基因表达而干预AS不稳定斑块的形成及破裂,具有良好的心血管保护作用。
[Abstract]:Aim: to observe the effect of Shuifeng decoction on Beclin-1 and Bcl-2 gene expression in atherosclerotic plaque of ApoE knockout mice. Methods: ApoE gene knockout mice model of unstable atherosclerotic plaque was established, and the mice were anesthetized to death after 13 weeks of anesthesia in blank group, model group, western medicine group, low, medium and high dose group. The changes of Beclin-1 and Bcl-2 gene expression in aortic tissue stained by HE were observed under optical microscope and semi-quantitative RT-PCR technique was used to detect the expression of Beclin-1 and Bcl-2 genes. Results: compared with the blank group, the expression level of Beclin-1 mRNA in the model group decreased significantly (P 0.01), and the expression level of Beclin-1 mRNA was increased in the western medicine group and the different dosage groups compared with the model group, while there was no significant difference in the expression level of Beclin-1 mRNA in the high dose Chinese medicine group compared with the western medicine group. Compared with the blank group, the expression level of Bcl-2 mRNA in the model group was significantly lower than that in the model group, compared with the model group, the level of Bcl-2 mRNA expression was increased in the western medicine group and the different dosage groups of the decoction, and there was no significant difference in the expression level of Bcl-2 mRNA between the high dose group and the western medicine group (P 0.05). Under the light microscope, the inflammatory reaction of the western medicine group and the different dose group were obviously weaker than that of the model group. Conclusion: the decoction may be able to regulate the expression of Beclin-1 and Bcl-2 in as vulnerable plaque by up-regulating the expression of Beclin-1 and Bcl-2 in ApoE gene knockout mice. It has a good cardiovascular protective effect on the formation and rupture of unstable plaque of as.
【作者单位】：辽宁中医药大学;辽宁中医药大学附属医院心内VIP科;
【基金】：中国博士后基金面上项目(20110491540) 辽宁省百千万人才项目(2011921022) 辽宁省自然科学基金项目(2014020055)
【分类号】：R285.5

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