炎症与氧化应激在大鼠动脉粥样硬化中的作用及药物干预研究

发布时间：2018-06-16 18:16

本文选题：炎症 + 氧化　；参考：《青岛大学》2014年硕士论文

【摘要】：目的：探讨相关炎症指标与氧化应激指标在大鼠早期动脉粥样硬化中的表达及应用瑞舒伐他汀(Rosuvastatin)、普罗布考(Probucol)干预后对各项指标的影响,并对两药作用进行比较。方法：雄性Wistar大鼠54只随机分5组,正常饮食对照组(A组)12只,高脂饮食模型组(B组)12只、瑞舒伐他汀干预组(C组)10只、普罗布考干预组(D组)10只、瑞舒伐他汀+普罗布考干预组(E组)10只。正常饮食对照组给予普通饲料喂养,高脂饮食模型组及药物干预组给予高脂饲料喂养同时给予卵清白蛋白(2.5mg/kg)腹腔注射(5次/周)。C、D、E组从第9周开始,进行药物干预。第16周末,所有大鼠称重,麻醉大鼠,开胸经右心房取血,检测血清血管内皮细胞钙黏蛋白(VE-cadherin)、脂联素(APN)、8-异前列腺素-F2a(8-iso-PGF2a)、超氧化物歧化酶(SOD)表达。在光镜下观察大鼠主动脉病理组织学改变。结果：与A组比较,模型组和药物干预组体重明显下降(P0.01),血清VE-cadherin、8-iso-PGF2a水平显著升高(P0.01),APN、 SOD水平降低(p0.01)；与B组比较,C、D、E组体重升高(P0.05)血清VE-cadherin、8-iso-PGF2a水平明显降低(p0.01), APN、SOD表达升高(P0.05),血管内膜损伤明显减轻；与C组比较,D、E组血清8-iso-PGF2a水平下降(P0.01),SOD水平升高(P0.01)；与C组比较,E组血清VE-cadherin降低(P0.01),APN升高(P0.01)；与D组比较,E组血清VE-cadherin降低(P0.05),APN升高(P0.05)；与CD组比较,E组血管内膜变薄(P0.05)。结论：瑞舒伐他汀与普罗布考均具有抗炎和抗氧化作用,普罗布考的抗氧化作用优于瑞舒伐他汀,两药联合使用可明显降低血清VE-cadherin、8-iso-PGF2a的水平,升高APN、SOD的表达,并可减轻血管内膜的病理改变。本实验可指导临床用药,在应用瑞舒伐他汀基础上加用普罗布考,可增强对血管内膜的保护作用。
[Abstract]:Aim: to investigate the expression of inflammatory and oxidative stress indexes in early atherosclerosis of rats and the effects of rosuvastatin (Rosuvastatin) and probucol (Probucoll) on these indexes and compare the effects of the two drugs. Methods: Fifty-four male Wistar rats were randomly divided into 5 groups: normal diet control group (n = 12), high fat diet group (n = 12), control group (n = 10), rosuvastatin group (n = 10) and probucol group (n = 10). Rosuvastatin probucol intervention group (n = 10). The normal diet control group was fed with normal diet, the high fat diet model group and the drug intervention group were given high fat diet and ovalbumin 2.5 mg / kg). At the end of the 16th week, all the rats were weighed and anesthetized, and the blood was taken from the right atrium through the right atrium. The expression of VE-cadherin, adiponectin (APN) 8-iso-PGF2aI, and superoxide dismutase (SOD) were detected. The histopathological changes of rat aorta were observed under light microscope. Results: compared with group A, body weight in model group and drug intervention group decreased significantly (P 0.01), serum VE-cadherinine 8-iso-PGF2a level increased significantly (P 0.01) and SOD level decreased (P 0.01). Compared with group B, the serum VE-cadherinine 8-iso-PGF2a level was significantly decreased, the expression of APN- SOD increased and the vascular intima injury was alleviated, compared with group C, the serum 8-iso-PGF2a level decreased, and the serum VE-cadherin decreased P0.01APN increased P0.01a, compared with C group, the serum VE-cadherin decreased P0.01APN increased P0.01a. Compared with group D, the serum VE-cadherin in E group decreased the level of P0.05APN increased, and that in group E was thinner than that in group CD. Conclusion: both rosuvastatin and probucol have anti-inflammatory and anti-oxidation effects. The antioxidant effect of probucol is better than that of rosuvastatin. The combined use of the two drugs can significantly reduce the level of serum VE-cadherinine 8-iso-PGF2a and increase the expression of APN- SOD. And can alleviate the pathological changes of vascular intima. This experiment can be used to guide the clinical use of Risuvastatin plus Probucol, which can enhance the protective effect on vascular intima.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R543.5

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