麝香保心丸防治动脉粥样硬化的实验研究

发布时间：2018-06-19 02:44

  本文选题：动脉粥样硬化 + 麝香保心丸　； 参考：《中药新药与临床药理》2015年04期

【摘要】：目的研究慢性炎症反应与动脉粥样硬化的关系及麝香保心丸防治动脉粥样硬化的作用机制。方法新西兰雄性大耳白兔60只,随机分为5组,即空白对照组,模型组,麝香保心丸低、高剂量组,立普妥组。高脂饲料造模4周后再连续给药6周,末次给药后(空腹大于12 h)耳动脉采血,麻醉后取主动脉,检测血脂及炎症因子,观察各组动物动脉内膜病理形态的改变,检测Ca2+/钙调蛋白依赖性蛋白激酶II(CaMKⅡ)蛋白表达及Ca MKII的活性。结果与模型组比较,麝香保心丸低、高剂量组及立普妥组的总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆红素(TBIL)、直接胆红素(DBIL)、肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、白细胞介素8(IL-8)水平及动脉粥样斑块数量、厚度均有明显降低,Ca MKII蛋白含量及活性均有明显下降,差异均有统计学意义(P0.05,P0.01)。结论麝香保心丸具有抗实验性新西兰大耳兔动脉粥样硬化的作用,并可能与降低CaMKⅡ蛋白含量或活性,减轻或抑制慢性炎症反应有关。
[Abstract]:Objective to study the relationship between chronic inflammation and atherosclerosis and the mechanism of Shexiang Baoxin Pill in preventing and treating atherosclerosis. Methods Sixty New Zealand male rabbits were randomly divided into 5 groups: blank control group, model group, low, high dose and Lipitor groups. After 4 weeks of high fat diet, the rats were given continuously for 6 weeks. After the last administration (fasting > 12 hours), the aorta was taken out, blood lipids and inflammatory factors were measured, and the pathological changes of intima of the arteries in each group were observed. The expression of Ca 2 / calmodulin dependent protein kinase II (CaMK 鈪，

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