脑室内注射orexin-A和orexin-B对下丘脑组胺释放的影响

发布时间：2018-06-20 01:49

本文选题：Orexin-A + Orexin-B　；参考：《临床麻醉学杂志》2017年09期

【摘要】：目的观察脑室内微注射orexin-A和orexin-B对下丘脑组胺释放的影响,为orexins促麻醉觉醒的机制提供新的解释。方法成年雄性SD大鼠随机分为三组:生理盐水组、orexin-A组和orexin-B组。在立体定位仪下将微透析管置入下丘脑结节乳头体核区。实验分为两部分:先收集脑室内分别注射orexin-A、orexin-B 1nmol和5nmol后1、2h和3h的微透析液(n=5),采用高效液相色谱法(HPLC)检测微透析液中组胺含量的时程变化。1周后,各组大鼠分别注入不同剂量的orexin-A和orexin-B(10、15nmol和20nmol,n=5),收集微注射后1h的透析液,检测组胺释放的剂量效应。在立体定位仪下结节乳头体核置入微注射管,1周后给予1.4%异氟醚(1 MAC)麻醉30min,各组大鼠分别微注射生理盐水、orexin-A和orexin-B 0.3μl(n=6),记录翻正反射恢复时间。结果与生理盐水组比较,脑室内微注射orexin-A 1nmol 2h后下丘脑组胺含量明显增加,但同样剂量的orexin-B无明显作用,注射orexin-A和orexin-B 5nmol后2、3h组胺含量均明显增加(P0.01)。脑室内微注射10nmol、15nmol和20nmol的orexin-A和orexin-B后1h组胺含量明显高于生理盐水,且20nmol含量最高(P0.05)。结节乳头体核微注射orexin-A后翻正反射恢复时间明显短于生理盐水(P0.01),微注射orexin-B后无明显作用。结论脑室内微注射orexin-A和orexin-B均可促进下丘脑组胺递质的释放,但orexin-A的作用更强。结节乳头体核微注射orexin-A促进异氟醚麻醉的觉醒。
[Abstract]:Objective to investigate the effects of intraventricular microinjection of orexin-A and orexin-B on histamine release from hypothalamus and to provide a new explanation for the mechanism of anesthesia arousal induced by orexins. Methods Adult male SD rats were randomly divided into three groups: normal saline group, orexin-A group and orexin-B group. The microdialysis tube was placed into the nucleus of the papillary body of the hypothalamic tubercle under the stereotactic locator. The experiment was divided into two parts: after intraventricular injection of orexin-Anoorexin-B 1nmol and 5nmol, the microdialysis solution was collected for 1h and 3h, respectively. The histamine content in microdialysis solution was measured by HPLC for 1. 1 weeks. Rats in each group were injected with different doses of orexin-A and orexin-BX 10nmol and 20nmol / nmol respectively. Dialysate was collected at 1 hour after microinjection to detect the dose effect of histamine release. Rats were anesthetized with 1.4% isoflurane 1 Macl for 30 min after microinjection of papillary body nucleus in tuberculous nucleus under stereotactic locator. Rats in each group were given physiological saline troxin-A and orexin-B 0.3 渭 l / L for 30 min, respectively. The recovery time of positive inversion reflex was recorded. Results compared with saline group, the content of histamine in hypothalamus increased significantly after intraventricular microinjection of orexin-A 1nmol for 2 h, but the same dose of orexin-B had no obvious effect. The content of histamine increased significantly at 2h after injection of orexin-A and orexin-B 5nmol. The content of histamine in orexin-A and orexin-B at 1 h after microinjection of 10 nmol and 15 nmol and 20nmol was significantly higher than that of normal saline, and the highest content of 20nmol was P0.05. The recovery time of positive inversion reflex after microinjection of orexin-A was significantly shorter than that of P0.01A, but it had no effect after microinjection of orexin-B. Conclusion Intraventricular microinjection of orexin-A and orexin-B can promote the release of histamine transmitters in hypothalamus, but orexin-A is more effective. Microinjection of orexin-A into nodular papillary nucleus promotes the arousal of isoflurane anesthesia.
【作者单位】：第四军医大学附属西京医院麻醉科;宁夏医科大学总医院麻醉科;
【基金】：国家自然科学基金(81371510) 国家自然科学青年基金(81401138)
【分类号】：R614

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