间歇运动对心梗大鼠心肌LIF及其受体表征和细胞凋亡的影响

发布时间：2018-06-20 01:50

本文选题：间歇运动 + 心肌梗死　；参考：《体育科学》2015年12期

【摘要】：目的:探讨间歇运动对心肌梗死(myocardial infarction,MI)大鼠心肌细胞凋亡和心肌白血病抑制因子(Leukemia inhibitory factor,LIF)及其受体LIFR表征的影响。方法:3月龄SD雄性大鼠,随机分为假手术组(Sham)、心梗安静组(MI)、心梗+间歇运动组(ME),每组12只。左冠状动脉前降支(Left anterior descending coronary artery,LAD)结扎建立MI模型,ME组采用小动物跑台进行8周间歇训练。训练结束后次日腹腔麻醉,血流动力学方法评定心功能,Masson染色观察分析心肌胶原容积(Collagen volume fraction,CVF)百分比,TUNEL法检测细胞凋亡,Western Blot检测心肌Bcl-2、Bax、LIF、LIFR、p-STAT 3和STAT 3蛋白表达,RT-qPCR检测心肌lif和lifr mRNA表达。结果:心梗大鼠心功能显著降低,心肌纤维化水平和细胞凋亡显著增加。间歇运动可有效改善心梗大鼠心功能,降低心肌纤维化水平,升高Bcl-2/Bax比值,减少TUNEL阳性颗粒,上调心肌LIF及LIFR蛋白与基因的表达和STAT 3磷酸化水平。结论:间歇运动可显著促进心梗大鼠心肌LIF蛋白表达,激活LIF/LIFR/STAT 3通路,抑制心肌细胞凋亡,改善心梗心脏病理重塑和心功能。开展运动干预缺血心脏LIF表征研究与心脏的保护效应,将为心梗患者运动康复手段筛选提供新思路。
[Abstract]:Aim: to investigate the effects of intermittent exercise on myocardial cell apoptosis and the characterization of myocardial leukemia inhibitory factor (inhibitory) and its receptor (LIFR) in myocardial infarction (MI) rats. Methods male Sprague-Dawley rats aged 3 months were randomly divided into sham-operated group, silent myocardial infarction group, and myocardial infarction intermittent exercise group, 12 rats in each group. Left anterior descending coronary artery (Left anterior descending coronary artery) ligation was used to establish MI model. The next day after the training, the abdominal cavity was anesthetized, The percentage of Collagen volume fractionation in Myocardial Collagen was evaluated by Hemodynamic Assay and Tunel method was used to detect apoptosis. Western Blot was used to detect the expression of Bcl-2LIFRP-STAT3 and STAT3 protein. RT-PCR was used to detect the expression of lif and lifr in myocardium. Results: cardiac function, myocardial fibrosis and apoptosis were significantly decreased in myocardial infarction rats. Intermittent exercise could effectively improve cardiac function, decrease myocardial fibrosis, increase Bcl-2 / Bax ratio, decrease Tunel positive granules, up-regulate the expression of LIF and LIFR protein and gene and phosphorylation of STAT3 in myocardium of myocardial infarction rats. Conclusion: intermittent exercise can significantly promote the expression of LIF protein, activate the LIF / LIFR / STAT3 pathway, inhibit cardiomyocyte apoptosis, and improve myocardial remodeling and cardiac function in myocardial infarction rats. The study of LIF characterization of ischemic heart with exercise intervention and the protective effect of the heart will provide a new idea for the screening of exercise rehabilitation methods in patients with myocardial infarction.
【作者单位】：陕西师范大学体育学院暨运动生物学研究所;马里兰大学医学院生物化学与分子生物学系;
【基金】：国家自然科学基金资助项目(31171141) 陕西师范大学特色学科建设项目(2015-01)
【分类号】：G804.7

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