毒扁豆碱对异氟醚麻醉大鼠炎性反应和认知功能的影响

发布时间：2018-06-23 16:04

本文选题：毒扁豆碱 + 异氟醚　；参考：《临床麻醉学杂志》2015年04期

【摘要】：目的探讨毒扁豆碱对异氟醚麻醉大鼠认知功能的影响及其机制。方法 2月龄SD大鼠80只,随机分为四组,每组20只。对照组(C组)吸入含有30%氧气的空氧混合气体6h;异氟醚组(I组)吸入1.4%的异氟醚6h;毒扁豆碱+异氟醚组(PI组)与毒扁豆碱组(P组)腹腔注射毒扁豆碱100μg/kg,每8小时一次,共3次。首次给药后,PI组吸入1.4%异氟醚6h;P组吸入同浓度载气6h。麻醉结束行动脉血气分析,12h后取海马组织,ELISA法检测TNF-α、IL-1β、IL-6含量,RTPCR法检测capase-3mRNA的表达。光镜下观察海马CA1区锥体细胞形态学变化。麻醉后第2~7天评估认知功能。结果四组大鼠动脉血气指标、血糖和海马TNF-α含量差异均无统计学意义。与C组和P组比较,I组和PI组大鼠海马IL-1β、IL-6含量、capase-3mRNA明显升高,第4~6天逃避潜伏期明显延长,探索时间明显缩短(P0.05)。与I组比较,PI组大鼠海马IL-1β、IL-6含量、caspase-3mRNA表达明显降低,第4~6天逃避潜伏期明显缩短,探索时间明显延长(P0.05)。结论毒扁豆碱改善了异氟醚麻醉引起的大鼠认知功能损害,其机制可能与抑制海马炎性反应及神经元凋亡有关。
[Abstract]:Objective to investigate the effect and mechanism of NGF on the cognitive function of isoflurane anesthetized rats. Methods 2 month old SD rats were randomly divided into four groups, 20 rats in each group. The control group (group C) inhaled 30% oxygen oxygen mixed gas 6h; isoflurane group (group I) inhaled 1.4% isoflurane 6h; toxic lentione + isoflurane group (PI group) and toxic eneninline group (group P) ) intraperitoneal injection of 100 mu g/kg, once every 8 hours, 3 times. After the first administration, group PI inhaled 1.4% isoflurane 6h; group P inhaled the same concentration of gas carrying gas 6h. anesthesia action pulse gas analysis, 12h after the hippocampus tissue, ELISA method to detect TNF- alpha, IL-1 beta, IL-6 content, RTPCR method for capase-3mRNA expression. Under light microscope observe pyramidal pyramidal hippocampus area under the light microscope to observe the pyramidal pyramidal area under the light microscope pyrampyramid area under the hippocampal microscopic observation pyrampyramid area pyramidal hippocamhippocampal area under the light microscopy observation pyrampyramid area pyramidal hippocamhippocampus under the light scope observation pyrampyramid area hippocamhippocamhippocampal details under the pyramidal observation of pyramidal Morphological changes. The cognitive function was evaluated on day 2~7 after anesthesia. Results there was no significant difference in the arterial blood gas index, blood glucose and TNF- alpha content in the four groups. Compared with group C and group P, the hippocampal IL-1 beta, IL-6 content, capase-3mRNA increased significantly in group I and PI group, and the escape latency was obviously prolonged on the day of 4~6, and the exploration time was obviously shortened (P) 0.05). Compared with group I, the hippocampal IL-1 beta, IL-6 content, caspase-3mRNA expression of rats in group PI were significantly reduced, the escape latency was significantly shortened and the exploration time was significantly prolonged (P0.05). Conclusion venom can improve the impairment of cognitive function in rats induced by isoflurane anesthesia. The mechanism may be related to the inhibition of hippocampal inflammatory response and neuronal apoptosis.
【作者单位】：兰州大学第一医院麻醉科;
【分类号】：R614

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