地格达-4味汤对“幽门结扎”性肝损伤的保护作用及机制研究

发布时间：2018-07-13 16:06

【摘要】：目的:研究地格达-4味汤对"幽门结扎"诱导的肝损伤的作用。方法:取SD大鼠50只,分成5组,即正常、模型、地格达-4味汤高、中、低(166.4、83.2、41.6 g·kg-1;生药量分别为557、278.6、139.3 g·kg-1)剂量组,共给药15 d,末次给药后禁食不禁水24 h,除正常以外腹腔注射10%水合氯醛麻醉,开腹幽门结扎,关腹后单笼饲养18 h,腹主动脉取血,取肝脏,测定相关指标。结果:模型组与正常组比较血清AST、ALT量明显增加(P0.01或P0.05),肝组织GSH-PX明显降低(P0.01);TBIL、DBIL、ALP明显增高(P0.01);形态学观察肝细胞有明显的脂肪变、水肿、边膜外肝细胞大量坏死;肝细胞膜蛋白MRP3表达上调至3.5倍、MRP4表达下调至0.05倍、核受体Car下调至2.8倍、核受体Pxr下调至0.0019倍。各给药组与模型组比较地格达-4味汤中、高剂量组AST、ALT明显降低,GSH-PX明显增高(P0.01);对TBIL、DBIL、ALP中剂量组明显降低(P0.05);肝组织形态有一定量的好转;中剂量组膜蛋白MRP3下调至0.3倍、MRP4上调至2.7倍、核受体Car上调至3066倍、Pxr上调至506倍。结论:模型建立成功,地格达-4味汤具有明显的保肝作用,且它的作用机制为对核受体Car、Pxr的作用,而调节MRP3、MRP4表达发生改变。据结果分析中剂量组效果最佳。
[Abstract]:Objective: to study the effect of Digda-4 decoction on hepatic injury induced by pyloric ligation. Methods: fifty Sprague-Dawley rats were divided into 5 groups: normal, model, high, middle and low (166.4N 83.2N 41.6 g kg-1) group, the dosage of raw drug was 557278.6139.3 g / kg-1, the dosage of digerda-4 was high, medium and low (166.4N 83.2N 41.6 g / kg -1), and the dosage was 557278.6139.3 g / kg-1. After 15 days of administration, fasting for 24 hours, 10% chloral hydrate was injected intraperitoneally to anesthetize, ligation of abdominal pylorus, feeding for 18 hours after abdominal closure. Blood was taken from abdominal aorta, liver was taken and related indexes were measured. Results: compared with the normal group, the serum alt level in the model group was significantly higher than that in the normal group (P0.01 or P0.05), while GSH-PX in the liver tissue was significantly decreased (P0.01) and the ALP in the liver tissue was significantly increased (P0.01). The expression of MRP3 was up-regulated to 3.5-fold. MRP4 was down-regulated to 0.05-fold. The expression of nuclear receptor car was down-regulated to 2.8-fold. The expression of nuclear receptor PXR was down-regulated to 0.0019 times. Compared with the model group, the ASTH-PX of the high dose group decreased significantly (P0.01), the middle dose group of TBILD DBILILALP decreased significantly (P0.05), the liver tissue morphology improved, the MRP3 decreased to 0.3 times and the MRP4 increased to 2.7 times. The nuclear receptor car was up-regulated to 3066 times and PXR was increased to 506-fold. Conclusion: the model was established successfully, and Digerda-4 decoction has obvious hepatoprotective effect, and its mechanism is the effect on nuclear receptor Carbonucleus Pxr, while the expression of MRP3 + MRP4 changes. According to the results, the medium dose group was the best.
【作者单位】：内蒙古民族大学;
【基金】：国家自然科学基金项目(81441105,81360673)
【分类号】：R29

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