NERC-300高频焊接仪在大鼠肝部分切除术中的应用研究
发布时间：2018-07-13 17:34
【摘要】：目的:本实验使用高频软组织焊接仪和超声刀行大鼠肝左外叶部分切除术,经测量手术时间、出血量、工作温度、热损伤范围、肝功能变化等指标,验证高频软组织焊接仪行肝部分切除的有效性和安全性。方法:1.标本选择:选取雄性Wistar大鼠48只,体重范围190-210g,给予普通饲料、自由饮水,适应性饲养1周后随机分组(实验组与对照组)。实验前禁食12h仅给予饮用水,在各项工作准备完成后,以5%水合氯醛7ml/kg腹腔注射麻醉行开腹肝左外叶部分切除术。2.最高温度测量:使用红外热像仪测量软组织高频焊接仪切割肝组织时产生的最高温度:3.手术时间:使用秒表计算开腹至关腹所用手术时间;4.出血量:采用术前及术后纱布称重的方法粗略比较两组大鼠术中的出血量,术中出血量(g)=术后纱布重量(g)-术前纱布重量(g),出血量(ml)=术中出血重量(g)/l。5.热损伤范围:术后分批处死大鼠,取第一天处死大鼠的肝脏切缘用甲醛缓冲液固定,石蜡包埋,苏木精-伊红(hematoxylin-eosin staining,HE)染色,由病理科医生观察病理变化并测量热损伤范围;6.大鼠肝功能变化:于实验前抽取各组大鼠眼内眦静脉血,于3000转/分钟离心后取血清,送至南方医科大学珠江医院检验科检测血清丙氨酸氨基转移酶(ALT),血清天冬门氨酸氨基转移酶(AST),并于术后1天、3天、7天分批处死后心脏取血再次送检。7..统计学方法:使用SPSS 20.0软件进行统计学分析,其中计量资料以x±s表示,采用SNK检验两两比较,采用t检验比较组间数据,P0.05表示差异有统计学意义。结果:1、手术时间:实验组平均手术时间为(9.05±0.25)min,对照组为(8.87±0.40)min,P0.05,二者无显著差异。2、术中平均最高温度:实验组术中平均最高温度为(78.63±8.86)℃,对照组术中平均最高温度为(125.35±9.52)℃,P0.05,二者差异明显,高频焊接仪术中最高工作温度低于超声刀。3、术中出血量:实验组术中平均出血量为(0.63±0.05)ml,对照组术中平均出血量为(0.61±0.05)ml,P0.05,二者无统计学差异。4、肝脏切缘热损伤范围:实验组肝脏切缘病理损伤范围为(1.13±0.26)mm,对照组肝脏切缘病理损伤范围为(1.69±0.29)mm,P0.05,二者差异明显,高频焊接仪组肝脏热损伤程度小于超声刀。5、术前术后肝功能变化情况:术后第一天及第七天,实验组与对照组肝脏AST、ALT变化程度比较P0.05,无统计学差异,第三天AST、ALT变化中,高频焊接仪组变化小于超声刀组,P0.05。结论:高频软组织焊接仪行Wistar大鼠肝脏左外叶部分切除术中具有一定的安全性和有效性。
[Abstract]:Objective: to study the effect of high frequency soft tissue welder and ultrasonic knife on the left lateral lobectomy of the rat liver, and to measure the operation time, blood loss, working temperature, the range of thermal injury and the changes of liver function. To verify the effectiveness and safety of partial hepatectomy with high frequency soft tissue welder. Method 1: 1. Specimen selection: 48 male Wistar rats were randomly divided into two groups (experimental group and control group). Drinking water was given for 12 hours before fasting. After the preparation of the work, 5% chloral hydrate 7ml/kg was injected intraperitoneally to perform partial resection of the left lateral lobe of the open liver. Maximum temperature measurement: use the infrared thermograph to measure the highest temperature produced by cutting liver tissue by using an infrared thermograph. Operation time: use stopwatch to calculate the operation time used to open to close abdomen. Bleeding volume: the intraoperative bleeding volume (g) of the two groups was roughly compared by gauze weighing before and after operation. The intraoperative bleeding volume (g) = postoperative gauze weight (g)-preoperative gauze weight (g),) bleeding volume (ml) = intraoperative bleeding weight (g) / L. 5). The range of heat injury: the rats were killed in batches after operation. The liver incised margin was fixed with formaldehyde buffer solution, embedded in paraffin, stained with hematoxylin-eosin staininghe. Pathological changes were observed by pathologists and the range of thermal injury was measured. The changes of liver function in rats: the blood samples were drawn from the intraocular canthus vein of rats in each group before the experiment, and the serum was collected after centrifugation at 3000 rpm. Serum alanine aminotransferase (alt) and aspartate aminotransferase (AST) were detected in the laboratory of Zhujiang Hospital of Southern Medical University. Statistical methods: SPSS 20.0 software was used for statistical analysis, in which the measurement data were expressed as x 卤s, SNK test was used to compare the two groups, and t test was used to compare the difference between groups (P0.05). Results: the average operative time was (9.05 卤0.25) min in the experimental group and (8.87 卤0.40) min in the control group (P 0.05). There was no significant difference between the two groups. The mean intraoperative maximum temperature was (78.63 卤8.86) 鈩，
本文编号：2120192
[Abstract]:Objective: to study the effect of high frequency soft tissue welder and ultrasonic knife on the left lateral lobectomy of the rat liver, and to measure the operation time, blood loss, working temperature, the range of thermal injury and the changes of liver function. To verify the effectiveness and safety of partial hepatectomy with high frequency soft tissue welder. Method 1: 1. Specimen selection: 48 male Wistar rats were randomly divided into two groups (experimental group and control group). Drinking water was given for 12 hours before fasting. After the preparation of the work, 5% chloral hydrate 7ml/kg was injected intraperitoneally to perform partial resection of the left lateral lobe of the open liver. Maximum temperature measurement: use the infrared thermograph to measure the highest temperature produced by cutting liver tissue by using an infrared thermograph. Operation time: use stopwatch to calculate the operation time used to open to close abdomen. Bleeding volume: the intraoperative bleeding volume (g) of the two groups was roughly compared by gauze weighing before and after operation. The intraoperative bleeding volume (g) = postoperative gauze weight (g)-preoperative gauze weight (g),) bleeding volume (ml) = intraoperative bleeding weight (g) / L. 5). The range of heat injury: the rats were killed in batches after operation. The liver incised margin was fixed with formaldehyde buffer solution, embedded in paraffin, stained with hematoxylin-eosin staininghe. Pathological changes were observed by pathologists and the range of thermal injury was measured. The changes of liver function in rats: the blood samples were drawn from the intraocular canthus vein of rats in each group before the experiment, and the serum was collected after centrifugation at 3000 rpm. Serum alanine aminotransferase (alt) and aspartate aminotransferase (AST) were detected in the laboratory of Zhujiang Hospital of Southern Medical University. Statistical methods: SPSS 20.0 software was used for statistical analysis, in which the measurement data were expressed as x 卤s, SNK test was used to compare the two groups, and t test was used to compare the difference between groups (P0.05). Results: the average operative time was (9.05 卤0.25) min in the experimental group and (8.87 卤0.40) min in the control group (P 0.05). There was no significant difference between the two groups. The mean intraoperative maximum temperature was (78.63 卤8.86) 鈩，
本文编号：2120192
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