伤科接骨片治疗大鼠骨折模型的影像学评价

发布时间：2018-07-17 04:49

【摘要】：目的借助于X-射线、CT扫描成像、MRI等医学成像技术评价中成药伤科接骨片对大鼠骨折模型的治疗作用。方法大鼠麻醉后手术分离右前肢桡骨,于中段形成横断的完全骨折,随后随机分为模型组、药物治疗组,另设伪手术组进行平行对照。治疗组按0.33g/kg的剂量灌胃给药,模型组和伪手术组等量灌胃生理盐水,连续给药4周,给药结束后以X射线、CT和MRI图像观察和记录骨折部位的愈合情况。最后麻醉处死动物,经解剖取骨折部位进行抗折力测定和HE染色的病理组织学检查。结果 X射线、CT和MRI检查结果清楚地表明,给药治疗后骨折部位可见有明显的致密性骨痂形成,骨折线模糊不清或消失,多数可见大量的钙盐沉积,趋于愈合,与骨折部位的病理组织学检查结果基本一致。结论借助于医学影像技术可以更加客观地评价药物对大鼠骨折模型的治疗作用,尤以CT四维成像技术更加直观、清晰,值得进一步地推广应用。愈合后的前肢桡骨的抗折力也明显地增强。
[Abstract]:Objective to evaluate the therapeutic effect of traditional Chinese medicine on fracture model of rats by X ray scanning CT scanning MRI and other medical imaging techniques. Methods the radial bone of right forelimb was separated from the right forelimb after anesthesia, and the transverse fracture was formed in the middle segment of the rat. Then the rats were randomly divided into the model group, the drug treatment group and the pseudo-operation group for parallel control. The treatment group was given intragastric administration of 0.33g/kg, and the model group and pseudo-operation group were given the same amount of normal saline for 4 weeks. The healing of fracture site was observed and recorded by X-ray CT and MRI images. Finally, anesthetized animals were killed and the fracture site was dissected for the determination of flexural strength and the histopathology of HE staining. Results the results of X-ray CT and MRI clearly showed that there was obvious dense callus formation in the fracture site, the fracture line was blurred or disappeared, and a large amount of calcium deposits were observed, which tended to heal. The results of histopathological examination were consistent with those of fracture site. Conclusion with the aid of medical imaging technique, the therapeutic effect of drugs on rat fracture model can be evaluated more objectively, especially the four-dimensional CT imaging technique is more intuitive and clear, which is worthy of further popularization and application. The flexural strength of the forelimb radius after healing was also significantly increased.
【作者单位】：江苏省中医药研究院;
【基金】：江苏省科技公益专项基金资助(编号:BM2007506)
【分类号】：S858.91

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