兔动脉粥样硬化过程中主动脉内膜水含量变化的研究

发布时间：2018-07-18 21:43

【摘要】：目的研究高脂饮食复制兔动脉粥样硬化过程中主动脉内膜水含量的变化。方法将45只新西兰大白兔随机分为普通饲料组(对照组)、高脂饲料组(高脂组)和高脂饲料加辛伐他汀组(辛伐他汀组),每组15只。在饲养的第8、16、24周末分别检测对照组、高脂组和辛伐他汀组血清中总胆固醇、甘油三酯和低密度脂蛋白胆固醇的浓度,HE染色观察主动脉的病理形态学变化并测量内膜厚度变化;将高脂组和辛伐他汀组主动脉弓内膜剥离,用真空冷冻干燥方法检测粥样硬化斑块内膜中水含量的变化。结果①高脂组血清中总胆固醇、甘油三酯和低密度脂蛋白胆固醇浓度均高于对照组和辛伐他汀组(P0.05)。②对照组主动脉内膜薄而光滑;高脂组动脉内膜明显增厚,光镜下可见大量泡沫细胞和脂质斑块;辛伐他汀组内膜局限性增厚,斑块局限,泡沫细胞数目减少、体积变小。③高脂饮食诱导兔形成动脉粥样硬化过程中,高脂组16周、24周与8周相比粥样硬化斑块内膜中水含量明显减少,24周与16周相比也明显减少(P0.05);辛伐他汀组16周、24周斑块内膜中水含量较高脂组相同时间点增多(P0.05)。结论兔动脉粥样硬化形成过程中,随时间延长粥样硬化斑块内膜中水含量减少。
[Abstract]:Objective to study the changes of aortic intima water content during high fat diet replication in rabbits with atherosclerosis. Methods Forty-five New Zealand white rabbits were randomly divided into three groups: normal diet group (control group), high-fat diet group (high-fat group) and high-fat diet plus simvastatin group (simvastatin group) with 15 rabbits in each group. The concentrations of total cholesterol, triglyceride and low density lipoprotein cholesterol in serum of control group, hyperlipidemia group and simvastatin group were detected by HE staining at the end of the 24th week of feeding. The pathological morphology and intimal thickness of aorta were observed by HE staining. The aortic arch intima of high fat group and simvastatin group were stripped and the changes of water content in intima of atherosclerotic plaque were detected by vacuum freeze drying method. Results 1 the concentrations of total cholesterol, triglyceride and low density lipoprotein cholesterol in serum of hyperlipidemia group were higher than those of control group and simvastatin group (P0.05). A large number of foam cells and lipid plaques were observed under light microscope. In simvastatin group, the intima was thickened, plaque was localized, the number of foam cells was reduced, and the volume was reduced. 3. 3 high fat diet induced the formation of atherosclerosis in rabbits. The water content in atherosclerotic plaque intima in high fat group was significantly lower than that in high fat group at 24 weeks and 8 weeks (P0.05), while that in simvastatin group was higher than that in high fat group at the same time point (P0.05). Conclusion during the formation of atherosclerosis, the water content in atherosclerotic plaque intima decreases with time.
【作者单位】：安徽医科大学附属省立医院心内科;
【分类号】：R543.5

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