丹参联合参附注射液对大鼠重症胰腺炎肾脏NF-κB调控的研究

发布时间：2018-07-23 17:39

【摘要】：目的观察丹参联合参附注射液干预对模型大鼠重症急性胰腺炎(SAP)肾脏核转录因子-κB(NF-κB)的表达、血清肌酐(Cr)、血清尿素氮(BUN)的影响,探讨其对SAP肾损害的作用机制,为临床运用丹参联合参附注射液治疗重症胰腺炎提供理论依据。方法将72只SD大鼠随机分为3组(假手术组、SAP模型组、SAP丹参联合参附治疗组),各组24只。建模成功后予以药物干预,分别在术后6、12及24 h将其麻醉,腹腔静脉采血检测血清Cr及BUN,免疫组织化学检测肾脏组织NF-κB蛋白的表达情况。结果 SAP建模后予以药物干预6、12、24 h后,SAP模型组、丹参联合参附治疗组同假手术组比较,NF-κB蛋白的表达增加,血清Cr、BUN升高(P0.01);丹参联合参附治疗组同SAP模型组相比较,NF-κB蛋白的表达以及血清Cr、BUN减少(P0.01);假手术组在不同时间NF-κB蛋白的表达以及血清Cr、BUN基本不变(P0.05);SAP模型组、丹参联合参附治疗组随着时间的推移,NF-κB蛋白的表达增加,血清Cr、BUN升高(P0.01)。光学显微镜下可见,丹参联合参附治疗组的胰腺、肾脏组织炎症较SAP模型组明显减轻。结论丹参联合参附注射液能够抑制SAP大鼠肾脏组织的NF-κB的表达,血清Cr、BUN一定程度上得到控制,阻断NF-κB的激活可能是SAP肾损害作用机制之一。
[Abstract]:Objective to observe the effects of salvia miltiorrhiza combined with shenfu injection on the expression of nuclear factor 魏 B (NF- 魏 B) in the kidney of (SAP) and the effect of serum creatinine (Cr), on serum urea nitrogen (BUN) in rats with severe acute pancreatitis, and to explore the mechanism of its effect on renal damage of SAP. To provide a theoretical basis for the treatment of severe pancreatitis with salvia miltiorrhiza combined with shenfu injection. Methods Seventy-two Sprague-Dawley rats were randomly divided into 3 groups (sham-operation group, SAP model group, SAP combined with salvia miltiorrhiza group) with 24 rats in each group. After the model was successfully established, the rats were anesthetized at 6h and 24h postoperatively, the serum Cr and BUNwere collected from abdominal vein and the expression of NF- 魏 B protein in renal tissue was detected by immunohistochemistry. Results the expression of NF- 魏 B protein was increased in SAP-treated group and salvia miltiorrhiza treatment group, compared with sham-operation group, after drug intervention for 6h and 12h after SAP modeling, the expression of NF- 魏 B protein was increased in salvia miltiorrhiza group and sham operation group. Compared with SAP model group, the expression of NF- 魏 B protein and serum Cr-bun decreased in salvia miltiorrhiza combined with shenfu group (P0.01), and the expression of NF- 魏 B protein and serum Cr-bun in sham-operation group were almost unchanged at different time points (P0.05). In salvia miltiorrhiza combined with shenfu treatment group, the expression of NF- 魏 B protein increased and serum Crnbun increased with time (P0.01). Under the optical microscope, the inflammation of pancreas and kidney in the salvia miltiorrhiza combined with shenfu group was significantly reduced than that in the SAP model group. Conclusion Salvia miltiorrhiza combined with shenfu injection can inhibit the expression of NF- 魏 B in renal tissue of SAP rats, and the inhibition of activation of NF- 魏 B may be one of the mechanisms of renal damage induced by SAP.
【作者单位】：贵阳中医学院第二临床医学院;贵阳中医学院第二附属医院;
【基金】：贵州省卫计委资助课题(gzwjkj2014-1-043)
【分类号】：R285.5

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