限制性液体复苏对失血性休克大鼠早期肺损伤的治疗作用

发布时间：2018-07-26 07:12

【摘要】：目的观察限制性液体复苏对失血性休克大鼠早期肺损伤的治疗作用。方法 SD大鼠60只,随机分为假手术对照组(C组)、限制性液体复苏组(LR组)、常规液体复苏组(TR组)、休克未复苏组(NR组),各15只。C组大鼠仅麻醉后行左侧颈动脉置管,右侧股静脉置管;后三组建立失血性休克模型后,LR、TR组分别给予限制性液体、常规液体复苏,NR组不做液体复苏。复苏结束后120 min处死大鼠取肺组织,光镜下观察肺组织病理改变,ELISA法检测肺组织中的核因子κB(NF-κB),免疫组化法检测肺组织中细胞黏附分子1(ICAM-1),末端脱氧核苷酸转移酶介导的原位末端标记法(TUNEL)检测肺组织细胞凋亡。结果 C组、LR组、TR组、NR组肺组织病理评分分别为(0.67±0.62)、(2.20±0.68)、(2.93±0.88)、(7.67±0.50)分,NF-κB水平分别为(0.76±0.09)、(1.16±0.10)、(1.26±0.13)、(3.07±0.19)U/m L,ICAM-1表达量分别为3.68±1.83、29.09±6.51、34.20±5.45、72.14±10.99,细胞凋亡指数分别为3.73±1.03、21.06±2.12、24.00±2.39、49.77±6.72。LR、TR组与C组比较,LR组与TR、NR组比较,P均0.05。结论限制性液体复苏对失血性休克大鼠早期肺损伤有治疗作用。
[Abstract]:Objective to observe the therapeutic effect of restrictive fluid resuscitation on early lung injury in hemorrhagic shock rats. Methods Sixty SD rats were randomly divided into three groups: sham operation control group (group C), restricted fluid resuscitation group (LR group), conventional fluid resuscitation group (tr group), shock and non-resuscitation group (NR group). In the latter three groups, the hemorrhagic shock model was established, and the LRR group was given restricted fluid, while the normal fluid resuscitation group did not do liquid resuscitation. The rats were killed at 120 min after resuscitation. Lung pathological changes were observed under light microscope. Nuclear factor 魏 B (NF- 魏 B) in lung tissue was detected by Elisa (NF- 魏 B), immunohistochemical method), cell adhesion molecule 1 (ICAM-1) in lung tissue was detected by immunohistochemistry, and apoptosis in lung tissue was detected by in situ terminal deoxynucleotidyl transferase-mediated terminal deoxynucleotidyl transferase (TDNtransferase) (TUNEL). 缁撴灉 C缁，

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