不同盖髓材料对大鼠磨牙牙髓组织病理学改变的实验观察
发布时间：2018-07-26 07:56
【摘要】：目的：通过比较Urbical LC光固化氢氧化钙、3M Adper Easy One自酸蚀粘结剂、Gluma脱敏剂及3M Vitrebond光固化玻璃离子在大鼠深龋模型中对牙髓组织的病理学变化，为临床深龋的树脂窝洞充填中如何选择盖髓材料起参考作用。 方法：选择河北医科大学实验中心提供Wistar雄性大鼠72只，体重250~300g,鼠龄5~6个月,口腔卫生状况良好,无明显龋坏、隐裂等牙体硬组织疾病。 根据不同盖髓方式随机分为A、B、C、D四组，每组18只。A组为对照组，B、C、D组为实验组 A组：Urbical LC光固化氢氧化钙 B组：3M Adper Easy One自酸蚀粘结剂 C组：Gluma脱敏剂 D组：3M Vitrebond光固化玻璃离子 A组：将大鼠100g/L水合氯醛(3mL/kg)腹腔注射麻醉后，仰卧固定，用30ml/L的饱和H2O2棉球清洁口腔，750mg/l乙醇随机消毒一侧磨牙区，选取该侧上颌第一磨牙为实验牙，用小球钻在实验牙合面预备近髓窝洞，以窝洞底部透红为标准。隔湿，干燥后，涂一薄层Urbical LC光固化氢氧化钙，光照20s，涂布3MAdper Easy One自酸蚀粘接剂20s，轻吹5s，光照20s，流动树脂充填。B组直接涂布粘接剂，流动树脂充填。C组涂布粘接剂前使用Gluma脱敏剂，D组涂布粘接剂前玻璃离子盖髓。B、C、D其余步骤同A。 分别于1d、7d、14d，，将大鼠于全麻下用4%多聚甲醛心脏灌流固定1h后，取实验牙,距根尖1mm处截断牙根，暴露牙髓,4%多聚甲醛固定48h,10%EDTA脱钙4～6周,梯度乙醇脱水、石蜡包埋,将标本按平行牙长轴方向做颊舌向连续切片，制备5μm厚切片，HE染色和Gram细菌染色。 采用Costa和Hebling的评分标准，评估包括炎症细胞反应、组织破坏程度、反应性牙本质形成和细菌染色四种组织学特征并计分，使用电子测量尺测量各组剩余牙本质厚度（RDT）。 结果： 1、术后1d，炎症反应中，各组之间差异具有统计学意义，P＜0.05，C组炎症反应明显较轻，与A、B、D三组比较有统计学意义，A、B、D三组之间差距无统计学意义。各组在组织紊乱、修复性牙本质形成及细菌渗透中差异无统计学意义。 2、术后7d，各组炎性反应明显减轻，各组间炎性反应、组织紊乱及修复性牙本质及细菌渗透差异无统计学意义。 3、术后14d，各组牙髓组织基本恢复正常，各组间炎性反应、组织紊乱、修复性牙本质形成及细菌渗透差异无统计学意义。 4、由于观察时间较短，各组未见明显修复性牙本质形成及细菌渗透。 5、炎性反应程度及组织紊乱程度与剩余牙本质厚度呈负相关。 结论： 1、Gluma脱敏剂可作为一种较好的盖髓材料，对牙髓组织刺激性较小。 2、Urbical LC光固化氢氧化钙组、3M Adper Easy One自酸蚀粘结剂、3M Vitrebond光固化玻璃离子之间对牙髓刺激无统计学差异，可认为其在对牙髓刺激方面相似。 3、随着时间的延长，各组炎症反应减轻，最后均能恢复正常。 4、牙髓炎症反应及组织紊乱程度与剩余牙本质厚度呈负相关。
[Abstract]:Objective: to compare the pathological changes of Urbical LC photocurable calcium hydroxide 3M Adper Easy One Adper Easy One desensitizer and 3M Vitrebond photocurable glass ion on pulp tissue in rats with deep caries. It plays a reference role in the selection of pulp capping material in resin cavity filling of deep caries. Methods: 72 male Wistar rats, weighing 250 ~ 300g, were selected from the experimental center of Hebei Medical University. The rats were 5 ~ 6 months old. The oral hygiene condition was good and there were no dental hard tissue diseases such as dental caries and cracked teeth. According to different ways of medullary capping, they were randomly divided into four groups. Each group of 18 rats. Group A is the control group. Group D is the experimental group A: Urbical LC light-cured calcium hydroxide group B: 3M Adper Easy One self-etching binder C group C group: Gluma desensitizer group D Group A: 100g/L hydrate (3mL/kg) was anesthetized by intraperitoneal injection. Supine fixation, 30ml/L saturated H2O2 cotton ball was used to clean oral cavity 750mg / l ethanol randomly. The maxillary first molars were selected as experimental teeth. The cavity near pulp fossa was prepared with small ball drill on the experimental occlusal surface, and the red penetration at the bottom of the cavity was taken as the standard. After wetting and drying, apply a thin layer of Urbical LC photocurable calcium hydroxide, illumination 20s, 3MAdper Easy One self-etching adhesive 20s, light blow 5s, illumination 20s, flow resin filling. B group direct coating adhesive. Before using Gluma desensitizer and group D, the other steps of vitreous ion pulp capping were the same as A. The rats were perfused with 4% paraformaldehyde for 1 hour under general anesthesia for 14 days. The experimental teeth were cut off from the root tip 1mm, and the pulp was fixed with 4% polyformaldehyde for 48 hours. The rats were decalcified for 46-week, gradient ethanol was dehydrated, paraffin was embedded. The buccal and tongue sections were made in parallel to the long axis of teeth, and 5 渭 m thick sections were prepared by HE staining and Gram bacterial staining. Costa and Hebling were used to evaluate the four histological features, including inflammatory cell reaction, tissue damage, reactive dentin formation and bacterial staining. (RDT). Was measured using an electronic ruler to measure the thickness of the remaining dentin in each group. Results: 1. In the inflammatory reaction at 1 day after operation, there was a significant difference between the three groups (P < 0.05). The inflammatory response in group C was significantly less than that in group A (P < 0.05), but there was no significant difference between group D and group A (P < 0.05). There was no significant difference in tissue disorder, formation of repaired dentin and bacterial permeation in each group. 2. On the 7th day after operation, the inflammatory reaction of each group was obviously alleviated, and the inflammatory reaction between groups was obviously alleviated. There was no significant difference in tissue disorder, repair dentin and bacterial osmosis. (3) on the 14th day after operation, the pulp tissue of each group returned to normal, inflammatory reaction and disordered tissue were observed in each group. There was no significant difference in the formation of prosthetic dentin and bacterial osmosis. 4. There was no obvious repair dentin formation and bacterial osmosis. 5. The degree of inflammatory reaction and the degree of tissue disorder were negatively correlated with the thickness of residual dentin. Conclusion: 1 Gluma desensitizer can be used as a better pulp capping material. 2Urbical LC photocurable calcium hydroxide group, 3M Adper Easy One self-etching binder and 3M Vitrebond photocurable glass ion had no significant difference on pulp stimulation. It can be considered that the pulp stimulation is similar. 3. With the prolongation of time, the inflammatory reaction of each group is reduced, and finally can return to normal. 4. The degree of inflammatory reaction and tissue disorder of dental pulp is negatively correlated with the thickness of residual dentin.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R783.1
本文编号：2145366
[Abstract]:Objective: to compare the pathological changes of Urbical LC photocurable calcium hydroxide 3M Adper Easy One Adper Easy One desensitizer and 3M Vitrebond photocurable glass ion on pulp tissue in rats with deep caries. It plays a reference role in the selection of pulp capping material in resin cavity filling of deep caries. Methods: 72 male Wistar rats, weighing 250 ~ 300g, were selected from the experimental center of Hebei Medical University. The rats were 5 ~ 6 months old. The oral hygiene condition was good and there were no dental hard tissue diseases such as dental caries and cracked teeth. According to different ways of medullary capping, they were randomly divided into four groups. Each group of 18 rats. Group A is the control group. Group D is the experimental group A: Urbical LC light-cured calcium hydroxide group B: 3M Adper Easy One self-etching binder C group C group: Gluma desensitizer group D Group A: 100g/L hydrate (3mL/kg) was anesthetized by intraperitoneal injection. Supine fixation, 30ml/L saturated H2O2 cotton ball was used to clean oral cavity 750mg / l ethanol randomly. The maxillary first molars were selected as experimental teeth. The cavity near pulp fossa was prepared with small ball drill on the experimental occlusal surface, and the red penetration at the bottom of the cavity was taken as the standard. After wetting and drying, apply a thin layer of Urbical LC photocurable calcium hydroxide, illumination 20s, 3MAdper Easy One self-etching adhesive 20s, light blow 5s, illumination 20s, flow resin filling. B group direct coating adhesive. Before using Gluma desensitizer and group D, the other steps of vitreous ion pulp capping were the same as A. The rats were perfused with 4% paraformaldehyde for 1 hour under general anesthesia for 14 days. The experimental teeth were cut off from the root tip 1mm, and the pulp was fixed with 4% polyformaldehyde for 48 hours. The rats were decalcified for 46-week, gradient ethanol was dehydrated, paraffin was embedded. The buccal and tongue sections were made in parallel to the long axis of teeth, and 5 渭 m thick sections were prepared by HE staining and Gram bacterial staining. Costa and Hebling were used to evaluate the four histological features, including inflammatory cell reaction, tissue damage, reactive dentin formation and bacterial staining. (RDT). Was measured using an electronic ruler to measure the thickness of the remaining dentin in each group. Results: 1. In the inflammatory reaction at 1 day after operation, there was a significant difference between the three groups (P < 0.05). The inflammatory response in group C was significantly less than that in group A (P < 0.05), but there was no significant difference between group D and group A (P < 0.05). There was no significant difference in tissue disorder, formation of repaired dentin and bacterial permeation in each group. 2. On the 7th day after operation, the inflammatory reaction of each group was obviously alleviated, and the inflammatory reaction between groups was obviously alleviated. There was no significant difference in tissue disorder, repair dentin and bacterial osmosis. (3) on the 14th day after operation, the pulp tissue of each group returned to normal, inflammatory reaction and disordered tissue were observed in each group. There was no significant difference in the formation of prosthetic dentin and bacterial osmosis. 4. There was no obvious repair dentin formation and bacterial osmosis. 5. The degree of inflammatory reaction and the degree of tissue disorder were negatively correlated with the thickness of residual dentin. Conclusion: 1 Gluma desensitizer can be used as a better pulp capping material. 2Urbical LC photocurable calcium hydroxide group, 3M Adper Easy One self-etching binder and 3M Vitrebond photocurable glass ion had no significant difference on pulp stimulation. It can be considered that the pulp stimulation is similar. 3. With the prolongation of time, the inflammatory reaction of each group is reduced, and finally can return to normal. 4. The degree of inflammatory reaction and tissue disorder of dental pulp is negatively correlated with the thickness of residual dentin.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R783.1
【引证文献】
相关期刊论文 前1条
1 赵千宁;王健平;佟玮玮;苏丹;余彦衡;;SonicFill超声树脂对大鼠成牙本质细胞和牙髓微血管的影响[J];中国组织工程研究;2016年16期
本文编号：2145366
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