七氟烷对糖尿病相关认知功能下降老年大鼠海马谷氨酸和γ-氨基丁酸的影响

发布时间：2018-08-02 16:25

【摘要】：目的观察七氟烷吸入麻醉后老年糖尿病大鼠的认知功能变化,并探索其神经递质谷氨酸(Glu)/γ-氨基丁酸(GABA)的变化规律。方法清洁级健康SD老年大鼠63只,高糖高脂饲料复合链脲佐菌素腹腔注射建立糖尿病模型,Morris水迷宫实验选取学习记忆能力下降的大鼠,观察吸入3%七氟烷1,2,3h后记忆能力的变化和海马组织中Glu和GABA的含量改变。结果建立糖尿病模型后,老年大鼠学习、记忆功能均下降,海马Glu降低,GABA升高,Glu/GABA比值降低(P0.05);吸入七氟烷后学习记忆能力、Glu/GABA比值降低更为明显(P0.05㖞,吸入3h后的差别更显著。结论长时间吸入高浓度(3%)七氟烷可进一步降低糖尿病相关认知功能降低的老年大鼠的记忆能力,其机制可能与海马中神经递质Glu和GABA失衡有关。
[Abstract]:Objective to observe the changes of cognitive function in aged diabetic rats after sevoflurane inhalation anesthesia and to explore the changes of glutamate (Glu) / 纬 -aminobutyric acid (GABA). Methods Sixty-three healthy SD rats with high glucose and high fat diet and streptozotocin were injected intraperitoneally to establish diabetes model, Morris water maze test was used to select the rats with impaired learning and memory ability. The changes of memory ability and the contents of Glu and GABA in hippocampus were observed after inhalation of 3% sevoflurane for 3 h. Results after the establishment of diabetic model, the learning and memory function of the aged rats decreased, the hippocampal Glu decreased and the Glu-P / GABA ratio decreased (P0.05), and the ratio of Glu-GABA / GABA decreased more significantly after sevoflurane inhalation (P0.05A, the difference was more significant after 3 h inhalation). Conclusion inhalation of high concentration (3%) of sevoflurane for a long time can further reduce the memory ability of the aged rats with impaired cognitive function associated with diabetes. The mechanism may be related to the imbalance of Glu and GABA in hippocampus.
【作者单位】：福建医科大学省立临床医学院福建省立医院麻醉科;三明市第二医院麻醉科;福建医科大学医学技术和工程学院;
【基金】：福建医科大学大学生创新创业训练计划项目(C13012)
【分类号】：R614

【参考文献】