桦褐孔菌对实验性Ⅱ型糖尿病治疗作用的研究

发布时间：2018-08-07 20:52

【摘要】：桦褐孔菌Inonotus obliquus (Fr.)Pilat是一种珍贵的药用真菌,在我国主要分布在黑龙江、吉林省的大兴安岭地区。国外学者对桦褐孔菌的活性成分进行了研究,并将其用于肿瘤、糖尿病、心血管疾病、肝病及艾滋病的防治中。目前我国学者对桦褐孔菌的研究还处于起步阶段。本课题首次对桦褐孔菌进行小鼠急性毒性实验研究,通过研究桦褐孔菌提取物对小鼠实验性Ⅱ型糖尿病机体氧化应激状态、慢性炎症反应以及糖脂代谢的影响,评价其药效并进一步研究药效物质基础。 采用STZ诱导实验性Ⅱ型糖尿病小鼠模型,以二甲双胍片、消渴灵片为阳性药,研究桦褐孔菌水提物及醇提物对Ⅱ型糖尿病小鼠的影响。连续给药4周,检测动物饮水量、采食量、体重和FBG值。末次给药后2h麻醉小鼠取血,用血细胞仪检测血全细胞分类,用全自动生化分析仪检钡(?)TC、TG、HDL、LDL,用酶标仪检测SOD、MDA、GSH-Px、 GSP、FINS,用ELISA法检测(?)TNF-α、IL.8、IL-10等相关指标。结果表明：桦褐孔菌水提物及醇提物均能缓解实验性Ⅱ型糖尿病小鼠的一般症状,降低FBG、FINS、GSP、TC、 TG、WBC和PLT,提高机体抗氧化能力,降低氧化产物MDA含量；抑制炎性反应,与模型对照组相比有差异显著。 采用水提醇沉法制取桦褐孔菌多糖,结合光电甩尾实验和小鼠体能评价实验,考察桦褐孔菌多糖对糖尿病的综合治疗作用,结果表明桦褐孔菌多糖能降低Ⅱ型糖尿病小鼠FBG、GSP、FINS,减少自发活动次数,缩短光电甩尾潜伏期,延长小鼠负重游泳力竭时间,与模型对照组相比有差异显著。 结论：桦褐孔菌提取物对实验性Ⅱ型糖尿病小鼠有显著的治疗作用,能提高小鼠体力和机体抗氧化能力,调节血脂代谢,抑制炎性反应,缓解外周神经病变；水提物的作用优于醇提物,其中水提物中的多糖成分有一定的降血糖作用,但其作用弱于水提物。
[Abstract]:Betula obliquus Inonotus obliquus (Fr.) Pilat is a precious medicinal fungus, which is mainly distributed in Heilongjiang and Daxing'anling area of Jilin Province. Foreign scholars have studied the active components of Betula obliquus and used them in the prevention and treatment of tumor, diabetes, cardiovascular disease, liver disease and AIDS. At present, the research on Betula obliquus is still in its infancy. In this study, the acute toxicity of Betula obliquus in mice was studied for the first time. The effects of the extract of Betula obliquus on oxidative stress, chronic inflammatory reaction and metabolism of glucose and lipid in mice with type 2 diabetes mellitus were studied. Evaluate its efficacy and further study its substance basis. The experimental model of type 2 diabetes induced by STZ was used to study the effects of water extract and alcohol extract of Betula platyphylla on type 2 diabetic mice with metformin tablets and Xiaokeling tablets as positive drugs. After 4 weeks of administration, the amount of water consumed, intake, body weight and FBG were measured. The blood samples were collected from anesthetized mice 2 hours after the last administration, the whole blood cell classification was detected by blood cell analyzer, the LDLs of TCG-TGG + HDLX, GSH-Px, GSPFINSwere detected by enzyme standard instrument, and the related indexes such as TNF- 伪 IL.8- IL-10 were detected by ELISA method. The results showed that the water extract and alcohol extract of Betula obliquus could relieve the general symptoms of experimental type 2 diabetic mice, decrease the Tc, TGG WBC and PLT, increase the antioxidant ability, decrease the content of MDA, and inhibit the inflammatory reaction. There was significant difference between the model control group and the model control group. The polysaccharides of Betula obliquus were prepared by water extraction and alcohol precipitation. The comprehensive therapeutic effect of polysaccharides from Betula obliquus on diabetes mellitus was investigated by combining the photoelectric tail flick test and the physical fitness evaluation test of mice. The results showed that the polysaccharides of Betula obliquus could decrease the number of spontaneous activities, shorten the latency of photoelectricity tail flick and prolong the exhausted time of swimming in mice with type 鈪，

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