NADPH氧化酶下调海马内微清蛋白表达在老年小鼠术后认知功能障碍中的作用
发布时间：2018-08-13 20:29
【摘要】：目的观察海马内微清蛋白(PV)表达变化在老年小鼠术后认知功能障碍(POCD)中的作用,并探讨磷酸酰胺腺嘌呤二核苷酸(NADPH)氧化酶是否参与其中。方法采用异氟醚麻醉+剖腹探查术建立POCD动物模型。雄性16月龄C57BL/6小鼠21只,随机均分为三组(n=7):对照+溶剂组(C+V组)、异氟醚麻醉+剖腹探查+溶剂组(POCD+V组)、异氟醚麻醉+剖腹探查+夹竹桃麻素组(POCD+A组)。POCD+A组术后1h腹腔注射夹竹桃麻素5mg/kg,其后每天注射一次直至术后第7天;其余两组注射等容量的溶剂。术后第6天行旷场实验和条件性恐惧实验训练,第7天在给予药物或溶剂后2h行条件性恐惧测试。行为学测试后2h取小鼠海马组织,采用Western blot检测PV、gp91phox、p22phox及羟基壬烯酸(4-HNE)的表达变化。结果三组小鼠总探索路程和中央格停留时间差异无统计学意义。与C+V组比较,POCD+V组在场景性条件恐惧测试中僵直时间明显缩短,海马PV表达明显下降,而gp91phox、p22phox和4-HNE表达明显升高(P0.05);与POCD+V组比较,POCD+A组在场景性条件恐惧测试中僵直时间明显延长,海马PV表达明显升高,gp91phox、p22phox和4-HNE表达明显降低(P0.05)。结论海马NADPH氧化酶活化介导PV表达降低可能参与老年小鼠POCD。
[Abstract]:Objective to investigate the role of microalbumin (PV) expression in postoperatively aged mice with cognitive dysfunction (POCD), and to explore whether (NADPH) oxidase of adenine dinucleotide is involved in it. Methods POCD animal model was established by isoflurane anesthesia. Twenty-one male 16-month-old C57BL/6 mice, They were randomly divided into three groups: control group (C V group), isoflurane anesthesia group (POCD V group), isoflurane anesthesia group (POCD A group). After 5 mg / kg, the rats were injected once a day until the 7th day after operation. The other two groups were injected with the same volume of solvent. The open field test and conditioned fear test were performed on the 6th day after operation, and the conditioned fear test was performed on the 7th day at 2 hours after the administration of the drug or solvent. Western blot was used to detect the expression of PVGp91phoxP22phox and hydroxylazenoic acid (4-HNE) in hippocampus of mice 2 h after behavioral test. Results there was no significant difference in total exploration distance and residence time between the three groups. Compared with C V group, POCD V group had significantly shorter stiffness time and lower hippocampal PV expression. Compared with the POCD V group, the expression of gp91phoxtrophox p22phox and 4-HNE was significantly increased (P0.05). Compared with the POCD V group, the rigidity time was significantly prolonged and the expression of p22phox and 4-HNE in hippocampus was significantly increased (P0.05). Conclusion the decrease of PV expression mediated by NADPH oxidase activation in hippocampus may be involved in POCD expression in aged mice.
【作者单位】： 南京大学医学院临床学院南京军区南京总医院麻醉科;
【基金】：国家自然科学基金(81271216,81300946)
【分类号】：R614
[Abstract]:Objective to investigate the role of microalbumin (PV) expression in postoperatively aged mice with cognitive dysfunction (POCD), and to explore whether (NADPH) oxidase of adenine dinucleotide is involved in it. Methods POCD animal model was established by isoflurane anesthesia. Twenty-one male 16-month-old C57BL/6 mice, They were randomly divided into three groups: control group (C V group), isoflurane anesthesia group (POCD V group), isoflurane anesthesia group (POCD A group). After 5 mg / kg, the rats were injected once a day until the 7th day after operation. The other two groups were injected with the same volume of solvent. The open field test and conditioned fear test were performed on the 6th day after operation, and the conditioned fear test was performed on the 7th day at 2 hours after the administration of the drug or solvent. Western blot was used to detect the expression of PVGp91phoxP22phox and hydroxylazenoic acid (4-HNE) in hippocampus of mice 2 h after behavioral test. Results there was no significant difference in total exploration distance and residence time between the three groups. Compared with C V group, POCD V group had significantly shorter stiffness time and lower hippocampal PV expression. Compared with the POCD V group, the expression of gp91phoxtrophox p22phox and 4-HNE was significantly increased (P0.05). Compared with the POCD V group, the rigidity time was significantly prolonged and the expression of p22phox and 4-HNE in hippocampus was significantly increased (P0.05). Conclusion the decrease of PV expression mediated by NADPH oxidase activation in hippocampus may be involved in POCD expression in aged mice.
【作者单位】： 南京大学医学院临床学院南京军区南京总医院麻醉科;
【基金】：国家自然科学基金(81271216,81300946)
【分类号】：R614
【共引文献】
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