七氟烷后处理对失血性休克猪肠黏膜AQP8和I-FABP表达的影响

发布时间：2018-08-28 16:25

【摘要】：目的观察七氟烷后处理对失血性休克巴马小型猪肠黏膜水通道蛋白8(AQP8)和血清肠脂肪酸结合蛋白(I-FABP)表达的影响,探讨七氟烷后处理对失血性休克引起的肠黏膜屏障损伤的保护作用及可能机制。方法 18只巴马小型猪随机分为对照组(S组)、失血性休克组(HS组)和七氟烷后处理组(Post/Sev组),每组6只。实验动物术前禁食8h,给予丙泊酚3mg/kg实施麻醉。S组麻醉后经股动脉和颈内静脉置管;HS组麻醉置管后建立失血性休克模型;Post/Sev组于麻醉置管建立失血性休克模型成功后给予2%七氟烷吸入30min。各实验组均于麻醉前(T0)及失血性休克后30min(T1)、1h(T2)、1.5h(T3)、2h(T4)、3h(T5)、4h(T6)时间点颈内静脉取血,ELISA法检测血清I-FABP的含量。失血性休克4h后放血处死实验动物,取肠组织制作病理切片,HE染色观察各组病理组织学变化;用干湿比法计算肠组织的含水量;ELISA法检测肠黏膜AQP8的表达水平。结果与S组相比,HS组和Post/Sev组血清I-FABP含量、肠黏膜AQP8表达水平和肠组织含水量均显著升高,差异有统计学意义(P0.05);与HS组比较,Post/Sev组上述指标明显降低,差异有统计学意义(P0.05)。光镜观察显示,S组肠黏膜未见明显变化;HS组肠黏膜损伤严重,可见肠黏膜出血、炎细胞浸润、上皮细胞坏死;Post/Sev组肠黏膜损伤明显减轻,仅见黏膜层腺体轻度扩张,上皮层和固有层中度分离,上皮下间隙轻度水肿,少许炎细胞浸润。结论七氟烷后处理可减轻失血性休克引起的肠黏膜屏障损伤,其机制可能与降低I-FABP和AQP8表达、减轻肠黏膜水肿有关。
[Abstract]:Objective to observe the effects of sevoflurane post-treatment on the expression of aquaporin 8 (AQP8) and fatty acid binding protein (I-FABP) in intestinal mucosa of Bama miniature pigs after hemorrhagic shock. To investigate the protective effect and possible mechanism of sevoflurane post-treatment on intestinal mucosal barrier injury induced by hemorrhagic shock. Methods 18 Bama miniature pigs were randomly divided into control group (S group), hemorrhagic shock group (HS group) and sevoflurane post-treatment group (Post/Sev group). Experimental animals fasted for 8 hours before operation. Propofol 3mg/kg was given to anesthetize. Group S was anesthetized by femoral artery and internal jugular vein. The model of hemorrhagic shock was established in HS group after intubation of femoral artery and internal jugular vein. 2% sevoflurane was inhaled for 30 min. Before anesthesia (T0) and 1 hour (T2) after hemorrhagic shock, the serum I-FABP levels were detected by Elisa at 3 h (T 5) and 4 h (T 6) after 30min (T 1) 1 h (T 2) and 1.5 h (T 3) 2 h (T 4) and 4 h (T 6). Four hours after hemorrhagic shock, the experimental animals were bled to death, and the histopathological changes of each group were observed by HE staining, and the water content of intestinal tissue was calculated by dry and wet ratio method and the expression level of AQP8 in intestinal mucosa was detected by Elisa. Results compared with S group, the levels of serum I-FABP, intestinal mucosal AQP8 expression and intestinal water content in HS group and Post/Sev group were significantly increased (P0.05), and those in HS group were significantly lower than those in HS group (P0.05). Light microscopic observation showed that there was no obvious change in intestinal mucosa in the S group. In HS group, there were severe intestinal mucosal damage, intestinal mucosal hemorrhage, inflammatory cell infiltration, epithelial cell necrosis and intestinal mucosal injury in Post- / Sev group. The mucosal glands were only slightly dilated. The epithelium and lamina propria were moderately separated, the upper subcutaneous space was slightly edematous and a few inflammatory cells infiltrated. Conclusion Post-treatment with sevoflurane can reduce the intestinal mucosal barrier injury induced by hemorrhagic shock, and its mechanism may be related to the reduction of I-FABP and AQP8 expression and the reduction of intestinal mucosal edema.
【作者单位】：沈阳军区总医院麻醉科;康平县人民医院麻醉科;
【基金】：全军“十二五”医学科研重点项目(BWS12J008)~~
【分类号】：R614

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