发育期Wistar大鼠鼻腔阻塞对硬腭生长发育的影响
发布时间：2018-09-19 11:26
【摘要】：目的呼吸方式的改变与颅颌面畸形存在着密切关系。本研究利用micro CT三维重建技术,评价生长发育期Wistar大鼠鼻腔阻塞对硬腭生长发育的影响。旨在为生长发育期鼻腔阻塞的青少年患者的矫治提供治疗参考依据。材料与方法本研究选取SPF(specific pathogen free,无特定病原体)级四周龄的Wistar大鼠30只,雄性。实验动物随机分为三组:双侧鼻阻塞组(张口呼吸组)、单侧鼻阻塞组(左侧)、对照组(未给予任何处理),每组老鼠10只。三组给予相同的饲养,同期行相同检查和处理。因大鼠的生长发育期为三周龄至十周龄,因此实验时间设定为49天,开始于大鼠三周龄,结束于大鼠十周龄。对所有老鼠进行称重。同一操作者使用同一 micro CT 扫描机(Skyscan 1076:Skyscan,Antwerp,Belgium)将大鼠麻醉后固定于仰卧位进行头颈部micro CT扫描获得原始数据,获得的micro CT数据以医学数字成像和通信(digital imaging and communications in medicine,DICOM)格式储存。共获取双侧鼻阻塞组数据、单侧鼻阻塞组数据及对照组数据三组数据。利用micro CT自带软件CTAn分别测量各大鼠硬腭骨密度(bone mineral density,BMD)、硬腭骨体积(bone volume,BV)和硬腭表面积(bone surface,BS)。将DICOM格式的数据导入Mimics17.0软件,确定图像的三维坐标方向,调节对比度。选取合适的菲尔德单位(Hounsfield units,HU)分离重建颅颌面骨骼三维图像,并分离出上颌骨三维图像。在硬腭上建立一个冠状观的测量网格,网格共含18个点。垂直于矢状面,分别经过上颌第一磨牙、上颌第二磨牙、上颌第三磨牙的近中解剖接触点创建3个测量水平(MLs),在这3个测量水平所对应的冠状面上分别定点,每个平面定6个点左边3个:左侧腭骨最高点(LH),左侧腭骨最低点(LL),左侧近中缝最高点(LM);右边3个:右侧腭骨最高点(RH),右侧腭骨最低点(RL),右侧近中缝最高点(RM)。分别测量各点处骨的厚度。为了测量的可靠性,两周后重复进行定点测量。使用SPSS19.0统计学软件对各测量数据进行配对t检验。结果1.实验组与对照组硬腭骨密度的比较双侧鼻阻塞组大鼠的平均硬腭骨密度为3.824±0.0008g/cm2,单侧鼻阻塞组的平均硬腭骨密度3.906±0.0016 g/cm2,对照组大鼠的平均硬腭骨密度为3.905±0.0029 g/cm2。双侧鼻阻塞组大鼠的硬腭骨密度相较于单侧鼻阻塞组和对照组大鼠的硬腭骨密度有明显减少(P0.05)。而单侧鼻阻塞组大鼠的硬腭骨密度与对照组大鼠的硬腭骨密度变化幅度较小,两组数据之间的差异无统计学意义(p0.05)。2.实验组与对照组腭骨厚度的比较双侧鼻阻塞组、单侧鼻阻塞组、对照组的硬腭骨厚度测量值之间无显著性差异的存在(P0.05)。在单个测量平面中,单侧(左侧或右侧)颚骨厚度,两边较厚,中间相对较薄,即接近额中缝处、及接近牙齿处颚骨相对较厚。3.实验组与对照组硬腭体积的比较双侧鼻阻塞组大鼠的平均硬腭体积为2150439.064±25419.23864 μm3,单侧鼻阻塞组的平均硬腭体积为2451010.931 ±55305.99638 μm3,对照组大鼠的平均硬腭体积为2817135.950±21169.73211 μm3。双侧鼻阻塞组、单侧鼻阻塞组、对照组的硬腭体积依次增大(P0.05)。4.实验组与对照组硬腭表面积的比较双侧鼻阻塞组大鼠的平均硬腭表面积为513731.6679±6679.34973 μm2,单侧鼻阻塞组的平均硬腭表面积为586816.7717±1216.05429 μm2,对照组大鼠的平均硬腭表面积为713360.1986± 17371.86358μm2。双侧鼻阻塞组、单侧鼻阻塞组、对照组的硬腭表面积依次增大(P0.05)。5.实验组与对照组大鼠体重的比较实验前大鼠平均体重为76.4±7.54 g,其中双侧鼻阻塞组为76.6±7.45 g,单侧鼻阻塞组为76.3 ±7.56 g,对照组为76.3 ±6.94g。实验结束时双侧鼻阻塞组大鼠平均体重为169.6±7.08 g,单侧鼻阻塞组为249.5±7.15 g,对照组为307.7±7.06 g。双侧鼻阻塞组、单侧鼻阻塞组、对照组大鼠体重依次增大(P0.05)。结论在Wistar大鼠生长发育过程中,鼻阻塞会导致大鼠硬腭体积、表面积的减小及体重减轻,但对硬腭骨厚度的发育没有明显影响。在双侧鼻阻塞时,大鼠硬腭骨密度有下降趋势。
[Abstract]:Objective To evaluate the effects of nasal obstruction on the growth and development of hard palate in growing Wistar rats by three-dimensional reconstruction of micro-CT. Thirty four-week-old Wistar rats of SPF (specific pathogen free) grade were randomly divided into three groups: bilateral nasal obstruction group (mouth-opening breathing group), unilateral nasal obstruction group (left side) and control group (without any treatment), with 10 rats in each group. All rats were weighed. The same operator used the same micro-CT scanner (Skyscan 1076: Skyscan, Antwerp, Belgium) to fix the rats in the supine position for head and neck MICR after anesthesia. The original data were obtained by o-CT scan, and the obtained micro-CT data were stored in the format of digital imaging and communication in medicine (DICOM). Bone mineral density (BMD), hard palate volume (BV) and hard palate surface area (BS). Direction of three-dimensional coordinates and contrast were determined by importing DICOM format data into Mimics 17.0 software. Appropriate Hounsfield units (HU) were selected to separate and reconstruct the three-dimensional skeleton images of craniomaxillofacial region. 3-D maxillary image. A coronal measurement grid was constructed on the hard palate with 18 points. Vertical to the sagittal plane, three measurement levels (MLs) were created by passing through the mesial anatomical contact points of the maxillary first molars, the maxillary second molars and the maxillary third molars, and each level was fixed on the coronal plane corresponding to the three measurement levels. Six points were fixed on the left side: the highest point of the left palate bone (LH), the lowest point of the left palate bone (LL), the highest point of the left near-middle suture (LM), the highest point of the right palate bone (RH), the lowest point of the right palate bone (RL) and the highest point of the right near-middle suture (RM). Results 1. Comparing the bone mineral density of hard palate between experimental group and control group, the average bone mineral density of hard palate in bilateral nasal obstruction group was 3.824.0008 g/cm 2, unilateral nasal obstruction group was 3.906.0016 g/cm 2 and control group was 3.905.0029 g/cm 2. Bone mineral density of hard palate in bilateral nasal obstruction group was significantly lower than that in unilateral nasal obstruction group and control group (P 0.05). Bone mineral density of hard palate in unilateral nasal obstruction group was smaller than that in control group, and there was no significant difference between the two groups (P 0.05). 2. There was no significant difference in the measurements of the thickness of the hard palate between the unilateral nasal obstruction group and the control group (P 0.05). 3. Comparing the hard palate volume between the experimental group and the control group, the average hard palate volume of the bilateral nasal obstruction group was 2150439.064 (+ 25419.23864 micron 3), the unilateral nasal obstruction group was 245110.931 (+ 55305.99638 micron 3), and the control group was 281735.950 (+ 21169.73211 micron 3). Comparing the hard palate surface area of the experimental group with that of the control group, the average hard palate surface area of the bilateral nasal obstruction group was 513 731.667 9 9 973 micron 2, the average hard palate surface area of the unilateral nasal obstruction group was 586 816.7717 (+ 1216.054 29 micron 2) and the control group was 713 360.1. The average body weight of the experimental group and the control group was 76.4+7.54 g, of which the bilateral nasal obstruction group was 76.6+7.45 g, the unilateral nasal obstruction group was 76.3+7.56 g, and the control group was 76.3+6.94 G. At the end of the test, the average body weight of rats in bilateral nasal obstruction group, unilateral nasal obstruction group and control group was 169.6 (+ 7.08 g), 249.5 (+ 7.15 g) and 307.7 (+ 7.06 g). The body weight of rats in bilateral nasal obstruction group, unilateral nasal obstruction group and control group increased in turn (P 0.05). Conclusion During the growth and development of Wistar rats, nasal obstruction can lead to the volume and surface area of the hard palate. The bone mineral density of the hard palate decreased with bilateral nasal obstruction.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R782
[Abstract]:Objective To evaluate the effects of nasal obstruction on the growth and development of hard palate in growing Wistar rats by three-dimensional reconstruction of micro-CT. Thirty four-week-old Wistar rats of SPF (specific pathogen free) grade were randomly divided into three groups: bilateral nasal obstruction group (mouth-opening breathing group), unilateral nasal obstruction group (left side) and control group (without any treatment), with 10 rats in each group. All rats were weighed. The same operator used the same micro-CT scanner (Skyscan 1076: Skyscan, Antwerp, Belgium) to fix the rats in the supine position for head and neck MICR after anesthesia. The original data were obtained by o-CT scan, and the obtained micro-CT data were stored in the format of digital imaging and communication in medicine (DICOM). Bone mineral density (BMD), hard palate volume (BV) and hard palate surface area (BS). Direction of three-dimensional coordinates and contrast were determined by importing DICOM format data into Mimics 17.0 software. Appropriate Hounsfield units (HU) were selected to separate and reconstruct the three-dimensional skeleton images of craniomaxillofacial region. 3-D maxillary image. A coronal measurement grid was constructed on the hard palate with 18 points. Vertical to the sagittal plane, three measurement levels (MLs) were created by passing through the mesial anatomical contact points of the maxillary first molars, the maxillary second molars and the maxillary third molars, and each level was fixed on the coronal plane corresponding to the three measurement levels. Six points were fixed on the left side: the highest point of the left palate bone (LH), the lowest point of the left palate bone (LL), the highest point of the left near-middle suture (LM), the highest point of the right palate bone (RH), the lowest point of the right palate bone (RL) and the highest point of the right near-middle suture (RM). Results 1. Comparing the bone mineral density of hard palate between experimental group and control group, the average bone mineral density of hard palate in bilateral nasal obstruction group was 3.824.0008 g/cm 2, unilateral nasal obstruction group was 3.906.0016 g/cm 2 and control group was 3.905.0029 g/cm 2. Bone mineral density of hard palate in bilateral nasal obstruction group was significantly lower than that in unilateral nasal obstruction group and control group (P 0.05). Bone mineral density of hard palate in unilateral nasal obstruction group was smaller than that in control group, and there was no significant difference between the two groups (P 0.05). 2. There was no significant difference in the measurements of the thickness of the hard palate between the unilateral nasal obstruction group and the control group (P 0.05). 3. Comparing the hard palate volume between the experimental group and the control group, the average hard palate volume of the bilateral nasal obstruction group was 2150439.064 (+ 25419.23864 micron 3), the unilateral nasal obstruction group was 245110.931 (+ 55305.99638 micron 3), and the control group was 281735.950 (+ 21169.73211 micron 3). Comparing the hard palate surface area of the experimental group with that of the control group, the average hard palate surface area of the bilateral nasal obstruction group was 513 731.667 9 9 973 micron 2, the average hard palate surface area of the unilateral nasal obstruction group was 586 816.7717 (+ 1216.054 29 micron 2) and the control group was 713 360.1. The average body weight of the experimental group and the control group was 76.4+7.54 g, of which the bilateral nasal obstruction group was 76.6+7.45 g, the unilateral nasal obstruction group was 76.3+7.56 g, and the control group was 76.3+6.94 G. At the end of the test, the average body weight of rats in bilateral nasal obstruction group, unilateral nasal obstruction group and control group was 169.6 (+ 7.08 g), 249.5 (+ 7.15 g) and 307.7 (+ 7.06 g). The body weight of rats in bilateral nasal obstruction group, unilateral nasal obstruction group and control group increased in turn (P 0.05). Conclusion During the growth and development of Wistar rats, nasal obstruction can lead to the volume and surface area of the hard palate. The bone mineral density of the hard palate decreased with bilateral nasal obstruction.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R782
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