小鼠背部皮肤的在体阻抗谱实验研究

发布时间：2018-10-08 07:11

【摘要】：皮肤中角质层与其他层面阻抗的巨大差异,使得将皮肤作为一个整体的阻抗特性研究较难开展。研究在体条件下无创或微创地检测与分析包含角质层的小鼠背部皮肤的阻抗特性。使用Agilent 4294A阻抗分析仪,测量麻醉状态下的15只C57BL/6小鼠背部皮肤的电阻抗频谱,使用表面电极和银针电极,分别对应皮肤和活性皮肤层阻抗数据。在整个实验频率范围(40~10~7Hz)内,随频率升高,皮肤阻抗幅值呈现-20dB/dec下降;活性皮肤层在40~10~3Hz之间幅值呈现-10 dB/dec下降,而在10~3~10~7Hz之间呈现-3dB/dec下降。皮肤相位随频率上升呈现V型曲线,而活性皮肤层相位呈现Π型曲线。低频时,皮肤阻抗完全由角质层阻抗组成,活性皮肤层的贡献可以忽略不计;当频率为10~5Hz时,活性皮肤层阻抗占皮肤的10%多,而在10~7Hz以上时,活性皮肤层阻抗占整个皮肤的80%以上。皮肤阻抗与活性皮肤层阻抗在10~3Hz以下具有显著性差异(P0.05),在10~4Hz以上无显著性差异。结果表明,表面电极与银针电极相结合的对比分析方法能够在微创前提下有效分析角质层在皮肤阻抗中的作用。
[Abstract]:The great difference of impedance between cuticle and other layers makes it difficult to study the impedance characteristics of skin as a whole. To study the noninvasive or minimally invasive detection and analysis of the impedance characteristics of mouse dorsal skin containing cuticle in vivo. Agilent 4294A impedance analyzer was used to measure the electrical impedance spectrum of the back skin of 15 C57BL/6 mice under anesthesia. The surface electrode and silver needle electrode were used to correspond to the impedance data of the skin and the active skin layer, respectively. In the whole experimental frequency range (40~10~7Hz), the skin impedance amplitude decreased by -20 dB / dec, and the active skin layer decreased by -10 dB/dec between 40~10~3Hz and -3 dB / dec between 10~3~10~7Hz. The phase of the skin showed V shape curve with the frequency rising, while the phase of the active skin layer showed a Pi shape curve. At low frequency, the skin impedance is completely composed of the cuticle impedance, and the contribution of the active skin layer is negligible. When the frequency is 10~5Hz, the active skin layer impedance accounts for more than 10% of the skin, but above the 10~7Hz, The impedance of active skin layer accounted for more than 80% of the whole skin. There was significant difference between skin impedance and active skin layer impedance below 10~3Hz (P0.05), but there was no significant difference above 10~4Hz. The results show that the comparative analysis method of surface electrode and silver needle electrode can effectively analyze the role of cuticle in skin impedance on the premise of minimally invasive.
【作者单位】：中国医学科学院北京协和医学院生物医学工程研究所;英国卡迪夫大学生物医学与生命科学学院口腔医院;
【基金】：国家自然科学基金仪器专项(81127003);国家自然科学基金(81171424)
【分类号】：R318

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