高氧液对大鼠急性一氧化碳中毒脑损伤保护作用的研究

发布时间：2018-10-15 13:23

【摘要】：目的:探讨不同剂量高氧液(hyperoxygenated solution,HOS)对急性CO中毒脑损伤的保护作用,为临床应用提供实验依据。方法:将30只SD大鼠随机分为5组,每组6只。正常组(N组)、中毒组(C组)和中毒治疗组(H组),H组再根据输注HOS剂量不同分为,HOS 10 ml/kg(H10组)、15 ml/kg(H15组)和20 ml/kg(H20组)。C组与H组大鼠经腹腔注入CO 120 ml/kg建立中毒模型,N组给予等量空气。各组大鼠分别在输注不同剂量的液体后即刻取血0.5 ml行血气检测,1 d抽取血3 ml进行神经元特异性敏感生化指标测定。实验结束后,所有动物均在麻醉状态下切取部分脑组织制作病理切片行病理学观察。结果:CO 120 ml/kg腹腔注射1.5 h能引起严重的低氧血症。中毒后1 d神经元特异性烯醇化酶、S-100β蛋白明显升高(P0.01)。中毒后24 h脑细胞出现明显水肿、间质增宽。中毒治疗组在静脉输注不同剂量的HOS后,动脉血氧分压(mm Hg)由43.04±4.13分别上升到69.56±3.41、77.11±2.49和81.65±3.85,脑组织病理学改变明显减轻,其中H20组降低最明显,具有显著的剂量依赖性。结论:HOS能明显提高CO中毒大鼠的Pa O2和动脉血氧饱和度,降低神经元特异性敏感指标的含量,对一氧化碳中毒脑损伤具有很好的保护作用,并具有剂量依赖关系。
[Abstract]:Objective: to investigate the protective effect of different doses of hyperoxia solution (hyperoxygenated solution,HOS) on brain injury caused by acute CO poisoning and to provide experimental evidence for clinical application. Methods: 30 SD rats were randomly divided into 5 groups with 6 rats in each group. Normal group (group N), poisoning group (group C) and poisoning treatment group (group H) were divided into HOS 10 ml/kg (H10 group), 15 ml/kg (H15 group), 20 ml/kg (H20). C group) and H group rats by intraperitoneal injection of CO 120 ml/kg. The blood samples of each group were collected immediately after infusion of different doses of liquid for blood gas detection. Blood samples were extracted for 3 ml on day 1 for the determination of specific sensitive biochemical indexes of neurons. At the end of the experiment, all animals were cut off part of brain tissue under anesthesia to make pathological sections for pathological observation. Results: CO 120 ml/kg intraperitoneal injection for 1. 5 h resulted in severe hypoxemia. The neuron-specific enolase and S-100 尾 protein increased significantly 1 day after poisoning (P0.01). At 24 h after poisoning, the brain cells showed edema and interstitial enlargement. The arterial partial pressure of oxygen (mm Hg) increased from 43.04 卤4.13 to 69.56 卤3.41 卤77.11 卤2.49 and 81.65 卤3.85 respectively in the poisoning group after intravenous infusion of different doses of HOS. The pathological changes of brain tissue were alleviated significantly in H20 group, which was significantly dose-dependent. Conclusion: HOS can significantly increase Pa O2 and arterial oxygen saturation in rats with CO poisoning, decrease the content of neuron specific sensitive indexes, and have a good protective effect on brain injury caused by carbon monoxide poisoning in a dose-dependent manner.
【作者单位】：西安医学院基础医学研究所;西安高氧医疗设备有限公司;华中科技大学同济医学院麻醉科;第四军医大学口腔医院麻醉科;
【基金】：国家自然科学基金(81471265) 陕西省科技统筹创新工程计划(2012KTCG01-02,2013KTZB03-03)
【分类号】：R595.1

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