和肾络法对肾间质纤维化miR-200a、miR-29c的影响
发布时间：2018-11-11 00:43
【摘要】：目的:观察和络泄浊颗粒对大鼠肾脏mi R-200a、mi R-29c的影响,探讨和法在肾间质纤维化的应用。方法:选雄性SD大鼠30只,随机分为Sham组(n=10)、UUO组(n=10)和UUO+REG组(n=10),手术采用单侧输尿管结扎术(UUO),以10%水合氯醛按0.35mg/kg腹腔注射麻醉实验大鼠后,取大鼠左侧腹部肾区切口,逐层分离并暴露左侧输尿管,以5-0缝合线结扎肾盏和肾下极处输尿管2道,并不离断输尿管,术中注意不碰伤肾包膜及周围组织,结扎输尿管后,肾置于原位;缝合腹膜、肌肉、皮肤。Sham组不结扎输尿管,其余操作步骤均与上述相同。术后予Sham组和UUO组生理盐水按10ml/kg.d-1灌胃,而UUO+REG组则给予生药含量为1g/ml和络泄浊颗粒按10g/kg.d-1灌胃。各组于术后第7天、第14天处死5只。每个大鼠左肾脏取出后,于低温下沿着肾脏纵切面(矢状面)分两半,一半肾脏组织用于RT-PCR检测mi R-200a和mi R-29c;另一半则用于HE染色、免疫组化检测α-SMA。结果:UUO成功建立慢性肾脏纤维大鼠模型。与Sham组相比,UUO组和UUO+REG组肾组织中α-SMA明显增多,其随时间的延长而增多。与Sham组相比,mi R-200a和mi R-29c在UUO组和UUO+REG组均表达下降,并随时间的延长,表达量逐渐降低;UUO+REG组高于UUO组表达量。在第7天和第14天,与UUO组相比较,mi R-200a在Sham组表达量有明显的统计学意义(P0.01);与UUO+REG组相比,与两组亦有差异(P0.05)。在第7天,与Sham组相比,其余两组mi R-29c表达量均较Sham组少(P0.05),UUO组与UUO+REG组在第7天无明显差异(P0.05),但在14天UUO组表达量少于Sham组和UUO+REG组(P0.05)。结论:以和法为指导思想运用和络泄浊颗粒可以增加肾脏组织mi R-200a和mi R-29c表达量,延缓肾间质纤维化。
[Abstract]:Aim: to observe the effect of Heluoxiezhuo granule (HXG) on renal mi R-200aFMI R-29c in rats and to explore the application of Hehe method in renal interstitial fibrosis. Methods: thirty male SD rats were randomly divided into Sham group (n = 10) and UUO REG group (n = 10). The experimental rats were anesthetized with 10% chloral hydrate and 10% chloral hydrate by unilateral ureteral ligation (UUO),). The left ureter was separated and exposed layer by incision of left abdominal and renal region in rats. The ureter was ligated with 5-0 suture line and the ureter at the lower pole of the kidney was not broken, and the renal capsule and surrounding tissues were not injured. After ligation of ureter, kidney was placed in situ. Peritoneum, muscle and skin were sutured. Ureter was not ligated in Sham group. The other procedures were the same as above. After operation, Sham group and UUO group were intragastrically perfused with normal saline as 10ml/kg.d-1, while in UUO REG group, the content of crude drugs was 1g/ml and Luoxiezhuo granule was given by 10g/kg.d-1. The rats in each group were killed on the 7th day and the 14th day after operation. After each rat left kidney was removed, it was divided into two halves along the longitudinal section (sagittal plane) of the kidney at low temperature. Half of the kidney tissue was used for RT-PCR detection of mi R-200a and mi R-29c.The other half was used for HE staining and immunohistochemistry for 伪 -SMA. detection. Results: chronic renal fiber rat model was successfully established by UUO. Compared with Sham group, 伪-SMA in renal tissue of UUO group and UUO REG group increased obviously and increased with time. Compared with Sham group, the expression of mi R-200a and mi R-29c decreased in both UUO group and UUO REG group, and the expression level of; UUO REG group was lower than that of UUO group with the increase of time. On the 7th and 14th day, the expression of mi R-200a in Sham group was significantly higher than that in UUO group (P0.01), and there was also significant difference compared with UUO REG group (P0.05). On the 7th day, compared with Sham group, the expression of mi R-29c in the other two groups was lower than that in Sham group (P0.05). There was no significant difference between), UUO group and UUO REG group on the 7th day (P0.05), but on the 14th day, the expression of R-29c in UUO group was lower than that in Sham group and UUO REG group (P0.05). Conclusion: the application of Heluoxiezhuo granule can increase the expression of mi R-200a and mi R-29c in renal tissue and delay renal interstitial fibrosis.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R692
[Abstract]:Aim: to observe the effect of Heluoxiezhuo granule (HXG) on renal mi R-200aFMI R-29c in rats and to explore the application of Hehe method in renal interstitial fibrosis. Methods: thirty male SD rats were randomly divided into Sham group (n = 10) and UUO REG group (n = 10). The experimental rats were anesthetized with 10% chloral hydrate and 10% chloral hydrate by unilateral ureteral ligation (UUO),). The left ureter was separated and exposed layer by incision of left abdominal and renal region in rats. The ureter was ligated with 5-0 suture line and the ureter at the lower pole of the kidney was not broken, and the renal capsule and surrounding tissues were not injured. After ligation of ureter, kidney was placed in situ. Peritoneum, muscle and skin were sutured. Ureter was not ligated in Sham group. The other procedures were the same as above. After operation, Sham group and UUO group were intragastrically perfused with normal saline as 10ml/kg.d-1, while in UUO REG group, the content of crude drugs was 1g/ml and Luoxiezhuo granule was given by 10g/kg.d-1. The rats in each group were killed on the 7th day and the 14th day after operation. After each rat left kidney was removed, it was divided into two halves along the longitudinal section (sagittal plane) of the kidney at low temperature. Half of the kidney tissue was used for RT-PCR detection of mi R-200a and mi R-29c.The other half was used for HE staining and immunohistochemistry for 伪 -SMA. detection. Results: chronic renal fiber rat model was successfully established by UUO. Compared with Sham group, 伪-SMA in renal tissue of UUO group and UUO REG group increased obviously and increased with time. Compared with Sham group, the expression of mi R-200a and mi R-29c decreased in both UUO group and UUO REG group, and the expression level of; UUO REG group was lower than that of UUO group with the increase of time. On the 7th and 14th day, the expression of mi R-200a in Sham group was significantly higher than that in UUO group (P0.01), and there was also significant difference compared with UUO REG group (P0.05). On the 7th day, compared with Sham group, the expression of mi R-29c in the other two groups was lower than that in Sham group (P0.05). There was no significant difference between), UUO group and UUO REG group on the 7th day (P0.05), but on the 14th day, the expression of R-29c in UUO group was lower than that in Sham group and UUO REG group (P0.05). Conclusion: the application of Heluoxiezhuo granule can increase the expression of mi R-200a and mi R-29c in renal tissue and delay renal interstitial fibrosis.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R692
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