右美托咪定对术后老龄小鼠海马组织白介素1β和肿瘤坏死因子α表达的影响

发布时间：2018-12-20 08:53

【摘要】：目的探讨右美托咪定对手术创伤后老龄小鼠海马组织白介素1β(IL-1β)和肿瘤坏死因子α(TNF-α)表达以及术后认知功能障碍(POCD)的影响。方法将105只老龄昆明小鼠随机分为4组。空白对照组(n=15):不实施麻醉和手术;麻醉药物组(n=30):腹腔注射氯胺酮100 mg/kg,持续吸入异氟醚60 min;麻醉药物+手术组(n=30):给予复合麻醉药物后实施剖腹探查脾切除手术;右美托咪定干预组(n=30):术前30 min各取15只小鼠分别给予右美托咪定15、25μg/kg腹腔内注射。术后第1日、第3日行Y迷宫实验(右美托咪定干预组在术后第3日进行),记录各组所需的训练次数;荧光定量PCR和Western blotting检测海马组织IL-1β、TNF-αmRNA及蛋白的表达。结果与空白对照组比较,麻醉药物组术后第1日训练次数增多(P0.05),术后第3日恢复正常(P0.05),麻醉药物+手术组术后第1日、第3日训练次数增多(P0.01,P0.05);术后第3日,右美托咪定15、25μg/kg干预组与空白对照组比较,差异均无统计学意义(P0.05)。与空白对照组比较,麻醉药物+手术组术后第1日、第3日IL-1β、TNF-αmRNA及蛋白的表达量均显著升高(P0.05或P0.01);麻醉药物组术后第1日、第3日及右美托咪定干预组术后第3日IL-1β、TNF-αmRNA及蛋白的表达量与空白对照组比较,差异无统计学意义(P0.05)。结论右美托咪定预处理能降低小鼠海马组织IL-1β、TNF-αmRNA及蛋白的表达,其改善小鼠POCD的作用与抑制海马炎症反应有关。
[Abstract]:Objective to investigate the effects of dexmetomidine on the expression of interleukin 1 尾 (IL-1 尾) and tumor necrosis factor 伪 (TNF- 伪) in hippocampus of posttraumatic aged mice and to investigate the effect of dexmetomidine on postoperative cognitive dysfunction (POCD). Methods 105 aged Kunming mice were randomly divided into 4 groups. Blank control group (nf15): no anesthesia and surgery; anesthetic group (nm30): ketamine 100 mg/kg, continuous inhalation of isoflurane for 60 min; The anesthetic group (n = 30) received laparotomy and splenectomy after combined anesthesia, and dexmetomidine intervention group (n = 30): 15 mice were treated with dexmetomidine 1525 渭 g/kg intraperitoneal injection 30 min before operation. Y-maze test was performed on the first and third day after operation (dexmetomidine intervention group was performed on the 3rd postoperative day). The training times were recorded and the expressions of IL-1 尾, TNF- 伪 mRNA and protein in hippocampal tissue were detected by fluorescence quantitative PCR and Western blotting. Results compared with the blank control group, the training times of the anesthetic group increased on the 1st day after operation (P0.05), returned to normal on the 3rd day after operation (P0.05), and increased on the 1st and 3rd day after operation in the anesthetic group (P0.01). P0.05); On the 3rd day after operation, there was no significant difference in dexmetomidine 1525 渭 g/kg intervention group and blank control group (P0.05). Compared with the blank control group, the expression of IL-1 尾, TNF- 伪 mRNA and protein in the anesthetic group on the 1st and 3rd postoperative day were significantly increased (P0.05 or P0.01). There was no significant difference in the expression of IL-1 尾, TNF- 伪 mRNA and protein between the anesthetic group and the blank control group on the 1st day, the 3rd day and the 3rd day after operation (P0.05). Conclusion dexmetomidine preconditioning can reduce the expression of IL-1 尾, TNF- 伪 mRNA and protein in hippocampus of mice. The effect of dexmetomidine on the expression of IL-1 尾, TNF- 伪 mRNA and protein is related to the inhibition of hippocampal inflammation.
【作者单位】：郑州大学药学院;郑州大学医药科学研究所;河南省直第三人民医院麻醉科;郑州大学第一附属医院麻醉科;
【基金】：河南省医学科技攻关计划项目(201304073)~~
【分类号】：R614

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