铁摄入过量对雄鼠组织损伤及雌鼠胚胎发育影响的实验研究

发布时间：2019-01-12 16:29

【摘要】：目的铁是人体必需的微量元素之一,但摄入过多可使机体氧化应激水平增强,造成一系列病理变化或损伤。本实验以大鼠为研究对象,探索铁摄入过量对组织损伤及胚胎发育的影响。方法雄性Wistar大鼠50只,随机分为5组并分别饲喂含不同剂量铁的饲料,贫铁组(X1组)：每日铁摄入量为1.67mg/kg体重,正常对照组(X2组)：每日铁摄入量为7mg/kg体重,低剂量组(X3组)：每日铁摄入量为21mg/kg体重,中剂量组(X4组)：每日铁摄入量为63mg/kg体重,高剂量组(X5组)：每日铁摄入量为189mg/kg体重。自由饮水,连续干预12周后处死,腹主动脉取血,用全自动生化仪检测血浆总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)、白/球比(A/G)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、尿素氮(BUN)、肌酐(CREA)、血红蛋白浓度等各项生化指标；剖取肝、脑、肾等脏器,检测血浆铁含量、肝、脑、肾铁含量等。雌性Wistar大鼠60只,随机分为5组：贫铁组(C1组)、正常对照组(C2组)、低剂量组(C3组)、中剂量组(C4组)、高剂量组(C5组),与雄鼠各组干预措施相同,6周后,与雄鼠合笼交配。怀孕雌鼠妊娠第20天称重,麻醉、剖取胎鼠,检查胎鼠的死胎数、吸收胎数、外观畸形数、着床数、体长、尾长等,制作胎鼠内脏和骨骼标本,检查内脏与骨骼的情况。结果随铁摄入剂量的升高,雄鼠血浆铁含量、血红蛋白浓度显著提高：肝脏铁含量、谷丙转氨酶、谷草转氨酶活性显著提高,血浆总蛋白、球蛋白含量显著降低；肾脏铁含量、血浆尿素氮、肌酐含量随着铁摄入剂量的增高而显著升高；各剂量组脑铁含量无显著差异。胎鼠出生情况检查显示,不同铁剂量组胎鼠体表颜色随铁剂量增加而逐渐变深,贫铁组胎鼠颜色较苍白,正常对照组淡红(正常),低剂量组较红润、中剂量组深红、高剂量组紫红；生长情况分析发现三个剂量组胎鼠的胎重、体长、尾长和胎盘重量随铁剂量的增加而下降；与正常对照组比较,胎重分别降低了13.3%、16.7%和17.2%(P值均0.05)；体长分别减少了3.8%、9.7%和10.0%,(P值均0.05)；尾长分别降低了3.6%、7.9%和5.0%(P值均0.05)；胎盘重分别降低了21.2%、23.1%和26.9%,(P值均0.05)；同时发现,低、中、高剂量组胎鼠与正常对照组比较,吸收胎数、椎骨色黑率、内脏出血率增加,中剂量组和高剂量组吸收胎数增加显著(P0.05),分别升高了5.88%和5.97%;椎骨色黑率增加显著(P0.05),分别升高了12.7%和12.9%;内脏出血率三个剂量组均显著增加(P0.05),分别提高了16%、27%和39%,随铁摄入量的增加而升高。结论雄性大鼠摄入过量的铁可使机体多组织、脏器铁含量提高,造成组织器官的损伤,使机体正常生理状况发生紊乱；雌性大鼠摄入过量铁可增加死胎、吸收胎甚至胚胎畸形的机率,导致胚胎发育毒性。因此铁过量的危害应引起关注。
[Abstract]:Objective Iron is one of the essential trace elements in human body, but excessive intake of iron can increase the level of oxidative stress and cause a series of pathological changes or injuries. In this study, the effects of excessive iron intake on tissue damage and embryonic development in rats were investigated. Methods 50 male Wistar rats were randomly divided into 5 groups and fed with different doses of iron. Iron deficiency group (X1 group): daily iron intake was 1.67mg/kg body weight, normal control group (X2 group): daily iron intake was 7mg/kg body weight. Low dose group (X3 group): daily iron intake was 21mg/kg body weight, medium dose group (X4 group): daily iron intake was 63mg/kg body weight, high dose group (X5 group): daily iron intake was 189mg/kg body weight. After 12 weeks of continuous intervention, blood was collected from abdominal aorta. Plasma total protein (TP), albumin (ALB), globulin (GLB), white / ball ratio (A / G) and alanine aminotransferase (ALT),) were detected by automatic biochemical instrument. Aspartate aminotransferase (AST), urea nitrogen (BUN), creatinine (CREA), hemoglobin concentration and other biochemical indicators; Liver, brain, kidney and other organs were dissected and plasma iron content, liver, brain and kidney iron content were measured. 60 female Wistar rats were randomly divided into 5 groups: iron deficient group (C1 group), normal control group (C2 group), low dose group (C3 group), middle dose group (C4 group) and high dose group (C5 group). Mate with male rat cage. On the 20th day of pregnancy, the female mice were weighed, anesthetized, the fetal rats were dissected, the number of dead fetus, the number of absorbed embryos, the number of appearance deformities, the number of implantation, the length of body, the length of tail and so on were examined. The viscera and bone specimens were made and the viscera and bone were examined. Results with the increase of iron intake dose, the plasma iron content and hemoglobin concentration of male rats were significantly increased: liver iron content, activity of alanine aminotransferase, glutamic oxaloacetic transaminase, plasma total protein and globulin decreased significantly. The contents of renal iron, plasma urea nitrogen and creatinine increased significantly with the increase of iron intake dose, but there was no significant difference in brain iron content among different dose groups. Birth examination of fetal mice showed that the body surface color of fetal mice in different iron dosage groups gradually became darker with the increase of iron dosage, the color of iron deficient group was paler, that of normal control group was light red (normal), that of low dose group was red and that of medium dose group was deep red, and that of iron poor group was lower than that of low dose group, and that of middle dose group was deep red. High dose group; Growth analysis showed that the fetal weight, body length, tail length and placental weight of the three dose groups decreased with the increase of iron dose. Compared with the normal control group, the fetal weight decreased by 13.3% and 17.2% (P < 0.05), the body length decreased by 3.8% and 10.0%, respectively (P < 0.05). The tail length was decreased by 3. 6% and 5. 0% (P < 0. 05), the placenta weight was decreased by 21. 2% and 26. 9% (P < 0. 05). At the same time, compared with the normal control group, the number of absorbed fetus, the rate of vertebral color and black, the rate of visceral hemorrhage in the low, middle and high dose groups increased significantly (P0.05). Increased 5.88% and 5.97% respectively; The rate of vertebral color and black increased significantly (P0.05), increased by 12.7% and 12.9%, and the rate of visceral hemorrhage increased significantly (P0.05), increased by 16% and 39%, respectively, and increased with the increase of iron intake. Conclusion excessive intake of iron in male rats can increase the content of iron in organs and tissues, and lead to injury of tissues and organs and disorder of normal physiological state of the body. Excessive iron intake in female rats can increase the chance of fetal death, absorption and even embryo malformation, leading to embryo development toxicity. Therefore, the harm of iron overdose should be concerned.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R153.1

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