泼尼松龙与地塞米松介导腰椎骨量降低的差异及其对成骨成脂基因表达的影响

发布时间：2019-01-29 03:55

【摘要】：目的:比较泼尼松龙(PRE)和地塞米松(DXM)诱导腰椎骨量丢失差异程度并利用成脂成骨经典信号通路探讨其可能机制。方法:选取3月龄SPF级雌性大鼠24只,随机分成4组:基线组(BL组)6只、年龄对照组(CON组)6只、泼尼松龙组(PRE组)6只、地塞米松组(DXM组)6只。BL组于实验开始时麻醉处死,其余3组正常喂养,其中CON组不作任何药物干预,PRE组予5mg/kg PRE每天一次皮下注射、DXM组以1mg/kg DXM每周两次皮下注射。干预3个月后麻醉下取材。取材时立即分离右侧股骨,获取髓腔内骨髓细胞进行涂片,油红"O"检测骨髓性状;取L6椎体作反转录式-聚合酶连锁反应(RT-PCR)检测骨保护素(OPG)及过氧化物酶体增殖物激活受体γ(PPAR-γ)表达;分离L1~L3椎体检测骨密度(BMD)。结果:在两种糖皮质激素(GC)分别干预3个月后,其BMD值均较CON组有明显下降(P0.05),DXM组下降更为显著(P0.01),且明显低于PRE组(P0.05)。DXM组骨组织OPG表达量明显低于其余三组(P0.05),但PPAR-γ则组间无明显差异。骨髓油红"O"涂片发现,两种GC组涂片阳性细胞数量多于BL组和CON组,但其组间无明显差异。结论:两种不同的糖皮质激素在诱导大鼠骨量丢失模型中,地塞米松诱导腰椎骨量减少的效果较泼尼松龙强,这可能与DXM通过OPG/RANKL/RANK信号通路中更大程度地抑制OPG表达有关。
[Abstract]:Aim: to compare the difference of bone mass loss induced by prednisolone (PRE) and dexamethasone (DXM) and to explore its possible mechanism by using the classical signal pathway of adipogenic osteogenesis. Methods: Twenty-four 3-month-old SPF female rats were randomly divided into four groups: baseline group (BL group, 6 rats), age control group (CON group, 6 rats) and prednisolone group (PRE group, 6 rats). Six rats in the dexamethasone group (DXM group) were anesthetized and killed at the beginning of the experiment. The other three groups were fed normally. The CON group received no drug intervention and the PRE group was subcutaneously injected with 5mg/kg PRE once a day. DXM group was subcutaneously injected with 1mg/kg DXM twice a week. After 3 months of intervention, the materials were taken under anesthesia. The right femur was separated immediately, bone marrow cells were obtained and smears were taken, and bone marrow characters were detected by oil red "O". The expression of osteoprotegerin (OPG) and peroxisome proliferator activated receptor 纬 (PPAR- 纬) was detected by reverse transcription-polymerase chain reaction (RT-PCR) in L6 vertebrae and bone mineral density (BMD). Was detected by separating L1~L3 vertebra. Results: after three months of intervention with (GC), the BMD values of both groups were significantly lower than those of CON group (P0.05), DXM group, P0.01). The expression of OPG in bone tissue of). DXM group was significantly lower than that of other three groups (P0.05), but there was no significant difference in PPAR- 纬 between groups. Bone marrow oil red "O" smear showed that the number of smear positive cells in GC group was more than that in BL group and CON group, but there was no significant difference between the two groups. Conclusion: in two different glucocorticoids induced bone loss models, dexamethasone can induce lumbar bone loss more effectively than prednisolone. This may be related to the greater inhibition of OPG expression by DXM through the OPG/RANKL/RANK signaling pathway.
【作者单位】：广州中医药大学;广州中医药大学第一附属医院二骨科;广州军区广州总医院博士后科研工作站;
【基金】：广东省教育厅学科建设专项基金(育苗工程)[2013LYM-0012] 广州中医药大学优秀青年学者科研基金项目(KAB110133K04)
【分类号】：R580

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