促红细胞生成素对SD大鼠坐骨神经损伤后Caspase-3和GAP-43表达及神经修复的影响

发布时间：2019-04-27 09:42

【摘要】：目的：探讨促红细胞生成素(EPO)对SD大鼠坐骨神经损伤后相应节段脊髓组织内Caspase-3和GAP-43表达情况及神经修复的影响，为临床治疗周围神经损伤提供实验性依据。方法：生长发育正常的洁净SD大鼠45只，雌雄不限，按照随机原则分为3个组，即对照组、模型组和实验组，每组15只。坐骨神经损伤模型制作方法：大鼠麻醉完全后取俯卧位固定，手术区域常规消毒、覆盖无菌单，在右侧大腿中部梨状肌下充分暴露坐骨神经后用普通外科持针器压榨坐骨神经2次，每次30秒钟，间隔10秒钟，损伤长度约为2mm。对照组正常饲养，模型组每日腹腔注射生理盐水，实验组每日腹腔注射促红细胞生成素。于术后的1w、2w、3w每组随机提取5只大鼠行坐骨神经功能指数检测，受损坐骨神经组织学观察，PCR方法检测相应节段脊髓组织内Caspase-3和GAP-43的表达情况。结果：坐骨神经功能指数检测显示对照组各时间段正常，，模型组与实验组显著降低，但第1w无明显差异（P〉0.05），第2w、3w实验组神经功能恢复明显优于模型组（P〈0.05）。HE染色显示对照组坐骨神经组织结构基本正常，神经纤维排列整齐、染色较均匀，组织未见明显水肿和炎细胞浸润；模型组的组织切片显示轴突肿胀明显、空泡变性，细胞核固缩，神经组织肿胀，炎细胞浸润明显；实验组同期病理表现较模型组明显减轻。相应节段的脊髓组织Caspase-3和GAP-43在对照组呈低表达状态，模型组和实验组在神经损伤后表达均增加，第1w两组表达情况差异较轻微，第2w、3w实验组Caspase-3表达较模型组明显减少，GAP-43实验组较模型组表达明显增加。结论：EPO可能通过抑制受损神经组织相应节段脊髓内Caspase-3的表达，促进GAP-43的表达，对SD大鼠坐骨神经损伤发挥保护、修复作用。
[Abstract]:Aim: to investigate the effect of erythropoietin (EPO) (EPO) on the expression of Caspase-3 and GAP-43 in the spinal cord of SD rats after sciatic nerve injury, and the effect of erythropoietin on nerve repair in order to provide experimental evidence for clinical treatment of peripheral nerve injury. Methods: 45 clean SD rats with normal growth and development were randomly divided into three groups: control group, model group and experimental group, with 15 rats in each group. The model of sciatic nerve injury was made: the rats were anesthetized completely and fixed in prone position, the operation area was routinely sterilized, and the sterile single was covered. After fully exposing the sciatic nerve under the piriform muscle in the middle part of the right thigh, the sciatic nerve was squeezed twice with a general surgical needle holder for 30 seconds at an interval of 10 seconds, and the length of the injury was about 2 mm. The model group was intraperitoneally injected with saline, and the experimental group was intraperitoneally injected with erythropoietin (Erythropoietin). At 1 week, 2 weeks and 3 weeks after operation, 5 rats in each group were randomly extracted for sciatic nerve function index detection, histological observation of injured sciatic nerve, and PCR method was used to detect the expression of Caspase-3 and GAP-43 in the corresponding spinal cord tissue. Results: the sciatic nerve function index showed that the control group was normal at each period of time, the model group and the experimental group decreased significantly, but there was no significant difference at the 1st week (P > 0.05), at the 2nd week, The recovery of nerve function in the experimental group was significantly better than that in the model group (P < 0. 05). HE staining showed that the sciatic nerve in the control group was basically normal, the nerve fibers were arranged neatly, the staining was more uniform, and there was no obvious edema and infiltration of inflammatory cells in the tissue. The histological sections of the model group showed obvious axonal swelling, vacuolar degeneration, nuclear shrinkage, nerve tissue swelling and inflammatory cell infiltration, and the pathological manifestations of the experimental group were significantly less than those of the model group at the same time. The expression of Caspase-3 and GAP-43 in the spinal cord tissue of the corresponding segments was low in the control group, and increased in both the model group and the experimental group after nerve injury. The difference between the two groups at the 1st week was slight, and the expression at the 2nd week was slightly different between the two groups. After 3 weeks, the expression of Caspase-3 in experimental group was significantly lower than that in model group, and the expression in GAP-43 group was significantly higher than that in model group. Conclusion: EPO may protect and repair sciatic nerve injury in SD rats by inhibiting the expression of Caspase-3 in the corresponding segments of the injured nerve tissue and promoting the expression of GAP-43.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R651.3

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