早期电针对局灶性脑缺血大鼠双侧皮质脑源性神经营养因子表达的影响

发布时间：2019-04-29 11:19

【摘要】：目的:观察早期电针治疗对局灶性脑缺血大鼠双侧皮质脑源性神经营养因子(BDNF-m RNA)的时序性变化,探讨电针治疗偏瘫的交叉迁移效果的分子生物学机制。方法:用线栓法建立局灶性脑缺血模型,将建模成功的54只SD大鼠随机分为健肢治疗组(UALTG)、患肢治疗组(ALTG)、对照组(CG),各组再随机分为1周、2周、3周组,造模成功24h后对治疗组浅麻醉行电针穴位治疗,每组各时间段结束24h内分离缺血皮质及对侧同部位皮质,采用RT-PCR法测定BDNF-m RNA表达量。结果:1对缺血皮质BDNF-m RNA表达的影响:ALTG组BDNF-m RNA在第7天、第14天、第21天时表达量均有增加,与CG组相比有显著性增高(P0.01),而UALTG组在第7天和第21天时表达量均有增加,与CG组相比有显著性增高(P0.01);在第7天和第21天时UALTG组较ALTG组表达量更高(P0.01)。2对缺血对侧皮质BDNF-m RNA表达的影响:ALTG组在第7天和第21天时BDNF-m RNA表达量高于CG组(P0.01);UALTG组在第14天时表达量高于CG组(P0.01),且ALTG组表达量同时也高于UALTG组(P0.01);而UALTG组和ALTG组在第21天时的表达量均下降,低于CG组(P0.01)。结论:早期电针治疗MCAO局灶性脑缺血大鼠健侧肢体和患侧肢体都能有效促使双侧皮质BDNF-m RNA的表达上调,具有明显的交叉迁移效果,且健侧较患侧更大幅度提高缺血皮质BDNF-m RNA的表达,这种变化可能是大鼠功能恢复较好和病死率较低的分子机制之一。
[Abstract]:Aim: to observe the sequential changes of brain-derived neurotrophic factor (BDNF-m RNA) in bilateral cortex of rats with focal cerebral ischemia and to explore the molecular biological mechanism of the effect of electroacupuncture on cross-migration of hemiplegia. Methods: the focal cerebral ischemia model was established by thread occlusion method. 54 SD rats were randomly divided into three groups: healthy limb treatment group (UALTG), affected limb group (ALTG), control group (CG), and then divided into three groups at random: 1 week, 2 weeks, 3 weeks group. At the end of 24 hours, the ischemic cortex and the contralateral cortex were separated from the ischemic cortex and the contralateral cortex within 24 hours. The expression of BDNF-m RNA was measured by RT-PCR method. Results: 1Effects on the expression of BDNF-m RNA in ischemic cortex: the expression of BDNF-m RNA in ALTG group increased on the 7th day, 14th day and 21st day, which was significantly higher than that in CG group (P0.01). The expression level in UALTG group was significantly higher than that in CG group on the 7th and 21st day (P0.01). On the 7th and 21st day, the expression of BDNF-m RNA in UALTG group was higher than that in ALTG group (P0.01). 2 the expression of BDNF-m RNA in ischemic contralateral cortex was higher in ALTG group than that in CG group on 7th and 21st day (P0.01). On the 14th day, the expression of UALTG group was higher than that of CG group (P0.01), and that of ALTG group was also higher than that of UALTG group (P0.01), while that of UALTG group and ALTG group was lower than that of CG group on the 21st day (P0.01). Conclusion: early electro-acupuncture treatment of MCAO focal cerebral ischemia rats can effectively promote the expression of BDNF-m RNA in both the contralateral limbs and the affected limbs, and it has obvious effect of cross-migration. The expression of BDNF-m RNA in ischemic cortex was significantly increased in the healthy side than in the affected side, which may be one of the molecular mechanisms of the better functional recovery and lower mortality in the ischemic cortex of rats.
【作者单位】：陕西省体育科学研究所;天津体育学院;天津市运动生理学与运动医学重点实验室;北京市第二人民医院;天津医科大学;澳大利亚南十字星大学;
【基金】：天津市高等学校科技发展基金计划项目(2006ZX02)
【分类号】：R245

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