不同浓度依托咪酯诱导人肝癌HepG2细胞体外凋亡的研究

发布时间：2019-05-24 06:23

【摘要】：目的探讨不同浓度的依托咪酯是否具有直接诱导人肝癌HepG2细胞凋亡的作用,寻找依托咪酯临床治疗的新途径。方法人肝癌细胞株HepG2体外扩增培养备用,选取不同浓度的依托咪酯E1、E2、E3和对照组(空白),分别培养12h、24h、48h后观察。结果与空白对照组相比,培养12h、24h、48h后E1组、E2组人肝癌细胞株HepG2细胞凋亡率无明显差异(P0.05),E3组凋亡率不断增加,具有显著性差异(P0.05);随着培养时间的延长及浓度的不断增加,E1与E2组对比人肝癌细胞株HepG2细胞凋亡率无明显差异(t=1.732,P0.05),E1与E3组对比人肝癌细胞株HepG2细胞凋亡率具有显著性差异(t=1.864,P0.05),E2与E3组对比人肝癌细胞株HepG2细胞凋亡率具有明显差异(t=1.691,P0.05)。结论依托咪酯在临床安全有效的浓度下不直接诱导人肝癌HepG2细胞的体外凋亡,浓度及时间达到一定峰值时可具有直接诱导人肝癌HepG2细胞体外凋亡的作用。
[Abstract]:Objective to investigate whether different concentrations of etomidate can directly induce apoptosis of human hepatocellular carcinoma HepG2 cells and to find a new way of clinical treatment of etomidate. Methods Human liver cancer cell line HepG2 was expanded and cultured in vitro. Different concentrations of etomidate E1, E2, E3 and control group (blank) were cultured for 12 hours, 24 hours and 48 hours respectively. Results compared with the blank control group, there was no significant difference in the apoptosis rate of human hepatocellular carcinoma cell line HepG2 in E1 group and E2 group 48 hours after culture for 12 hours, but the apoptosis rate in E3 group increased significantly (P 0.05). With the prolongation of culture time and the increase of concentration, there was no significant difference in apoptosis rate between E1 group and E2 group (t 鈮，

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