蒺藜提取物提高大强度耐力运动大鼠的运动能力及其机制

发布时间：2019-06-17 18:58

【摘要】：1.目的研究蒺藜提取物对大强度耐力运动大鼠运动能力的影响及其机制。 2.方法 32只SD大鼠随机分为4组：安静对照组（C，n=8）、蒺藜组（TT，n=8）、运动组（E，n=8）和蒺藜+运动组（TT+E，n=8）。安静对照组和运动组大鼠自由饮用纯水，而蒺藜组和蒺藜+运动组大鼠自由饮用蒺藜提取物配成的蒺藜饮料，记录每日大鼠的饮用量（大鼠摄取的蒺藜提取物在120mg/kg体重左右）。运动大鼠进行大强度的五周递增负荷运动，每周运动6天。5周耐力运动的倒数第二天进行30min的强度为30m/min的跑台运动，断尾取血，检测运动前和运动后30min、2h的血糖水平。5周耐力运动的最后一天，各组大鼠进行一次力竭运动。力竭运动结束后36h，大鼠麻醉后下腔静脉取血，检测血浆睾酮(Testosterone，T)水平、胰岛素样生长因子1（Insulin-like growth factor1，IGF-1）水平、血红细胞数（RBC）、血红蛋白含量（Hb）。大鼠处死后取腓肠肌、比目鱼肌，秤重，用BCA试剂盒检测总蛋白含量；用western blot方法检测雄激素受体（Androgenreceptor，AR）、胰岛素样生长因子1受体（Insulin-like growth factor receptor，IGF-1R）和肌球蛋白重链（Mysion heavy chain，MHC）的蛋白水平。 3.结果 1）蒺藜提取物提高大强度耐力运动大鼠的运动能力（1）蒺藜提取物减轻大强度耐力运动大鼠体重降低的程度实验前各组大鼠体重无显著性差异。从第三周开始，运动组、蒺藜+运动组大鼠的体重显著低于安静对照组（p0.05），该作用一直持续至实验结束（p0.01）。与运动组比较，蒺藜+运动组大鼠的体重在第五周时显著增加（p0.05）。此外，蒺藜组大鼠的体重与安静对照组相比，在各周都无显著性差异。（2）蒺藜提取物提高大强度耐力运动大鼠的一次力竭运动时间与对照组相比，蒺藜组大鼠的一次力竭运动时间无显著差异。五周耐力运动后，蒺藜+运动组大鼠的一次力竭运动时间比运动组大鼠显著延长（p0.01），约延长了63.2%。此外，运动训练大鼠的一次力竭运动时间比对照组显著延长（p0.05）。 2）蒺藜提取物提高大强度耐力运动大鼠运动能力的机制（1）蒺藜提取物提高大强度耐力运动大鼠的血浆睾酮水平和腓肠肌AR的蛋白水平与安静对照组相比，运动组大鼠的血浆睾酮水平显著降低（p0.01）；蒺藜+运动组大鼠的睾酮水平与运动组比较显著增加（p0.01），与对照组相比无显著差异，表明蒺藜提取物能完全逆转大强度运动所造成的血睾酮水平降低。此外，与安静组相比较，运动组大鼠腓肠肌的AR蛋白含量无显著性差异。蒺藜+运动组大鼠腓肠肌的AR蛋白表达水平比运动组的显著升高（p0.05）。（2）蒺藜提取物提高大强度耐力运动大鼠的血浆IGF-1水平和腓肠肌IGF-1R的蛋白水平与安静对照组相比，运动组大鼠血浆的IGF-1的水平没有显著性差异。蒺藜+运动组大鼠的IGF-1水平与运动组相比较显著增加（p0.05），表明补充蒺藜提取物能提高大强度运动大鼠血浆的IGF-1水平。此外，运动组与安静组相比较，腓肠肌IGF-1R蛋白含量没有显著性差异，蒺藜+运动组与运动组相比较，腓肠肌IGF-1R蛋白表达水平显著升高（p0.05）。（3）蒺藜提取物增加大强度耐力运动大鼠腓肠肌的总蛋白浓度和MHC的蛋白水平与对照组相比，运动组大鼠比目鱼肌和腓肠肌的相对重量均显著增加、且腓肠肌的蛋白浓度也显著增加；而蒺藜组大鼠的比目鱼肌和腓肠肌的相对重量、蛋白浓度均无显著差异。与运动组比较，蒺藜+运动组大鼠腓肠肌的蛋白浓度显著增加、但腓肠肌的相对重量以及比目鱼肌的蛋白浓度都没有显著性差异，而比目鱼肌相对重量却比较意外地下降（p0.05）。此外，与安静组相比，运动组的MHC蛋白水平没有显著性差异。与运动组相比，蒺藜+运动组的MHC蛋白水平显著增高（p0.05）。（4）藜提取物增加大强度耐力运动和不运动大鼠的一次力竭运动后30min的血糖水平蒺藜提取物能降低运动和不运动大鼠运动前的血糖水平。蒺藜组与安静组比较、蒺藜+运动组与运动组比较，血糖水平在一次力竭运动后的30min显著增加（分别是p0.05，p0.01），但在运动后2h，各组血糖水平无显著差异。（5）蒺藜提取物对大强度耐力运动和不运动大鼠的Hb、红细胞数无影响 4.结论： 1）蒺藜提取物延长大强度耐力运动大鼠的一次力竭运动时间，提高其运动能力。 2）蒺藜提取物增强大强度耐力运动大鼠运动能力的机制可能与其增加睾酮/AR水平和IGF-1/IGF-1R水平，，从而使腓肠肌的蛋白浓度、MHC蛋白水平增加，以及增加一次力竭运动后30min的血糖水平有关。 3）蒺藜提取物增强大强度耐力运动大鼠运动能力的机制与Hb和RBC数无关。
[Abstract]:1. Purpose The effect of the extract of the extract on the exercise ability of the large-strength endurance exercise rats and its machine System. 2. Methods 32 SD rats were randomly divided into 4 groups: the quiet control group (C, n = 8), the control group (TT, n = 8), the exercise group (E, n = 8), and the Astragalus + exercise group (TT + E). , n = 8). The quiet control group and the exercise group rats were free to drink pure water, while the rats in the control group and the exercise group were free to drink pure water, and the drinking quantity of the daily rats (the rat's uptake of the ginkgo leaf extract at 120 mg/ kg) was recorded. body weight). The exercise rats were subjected to five-week incremental load exercise with a large intensity, and the exercise was carried out for 6 days a week. The last day of the 5-week endurance exercise was performed with a treadmill exercise of 30 m/ min for 30 minutes, the blood glucose level of 2 hours was measured at 30 min and 2 hours before and after the exercise, and the 5-week endurance exercise One day of the last day, each group of rats The level of serum testosterone (T), insulin-like growth factor 1 (IGF-1), blood red blood cell count (RBC) and hemoglobin were measured at 36 h after the end of exhaustive exercise. (Hb). After the rats were sacrificed, the gastrocnemius muscle, the flounder muscle and the weight were weighed, and the total protein content was detected by the BCA kit; the androgen receptor (AR), the insulin-like growth factor receptor (IGF-1R) and the myosin heavy chain (MHC) were detected by western blot. protein water Ping.3. Results 1) The extract of the extract of the extract of the invention has the advantages of high strength, The exercise ability (1) of the force-moving rats to reduce the high-strength resistance to the extract Experimental study on the degree of weight loss in rats with force exercise There was no significant difference in the weight of the rats in the first group. From the third week, the weight of the rats in the exercise group and the group of the rats of the positive group was significantly lower than that of the quiet control group (p0.05), and the weight of the rats was always the same as that of the control group (p0.05). continued until the end of the experiment (p0.01). In comparison with the exercise group, the body weight of the baboon + exercise group was at the fifth Significant increases at week (p0.05). In addition, the body weight and the quiet control group in the treatment group There was no significant difference in each week. The time of exhaustive exercise of endurance sports rats was compared with that of the control group. There was no significant difference in the time of exhaustive exercise of the rats in the group. After the five-week endurance exercise, the time of exhaustive exercise of the rats in the group of exercise group and exercise group was significantly longer than that of the rats in the exercise group. (p0.01), about 63.2%. In addition, the exercise training rats' exhaustive exercise The time was significantly longer than that in the control group (p0.05). A mechanism (1) to improve the exercise capacity of a large-strength endurance exercise rat by taking the object (1) to improve the strength and endurance of the extract Plasma Testosterone in Exercise Rats Compared with the control group, the level of serum testosterone and the level of serum testosterone in the control group were significantly lower than that of the control group (p0.01), and the level of testosterone in the rats of the group of the rats of the group of the group of the rats of the group of the group of the group of the group of the group of the group of the group of the control group was significantly higher than that of the control group (p0.01), and there was no significant difference in the level of testosterone and the control group, indicating that the level of the serum testosterone of the rats in the exercise group The product can completely reverse the level of blood testosterone caused by the large-intensity exercise. In addition, compared with the quiet group, There was no significant difference in the AR protein content of the gastrocnemius in the exercise group. The expression of R-protein was significantly higher than that of the exercise group (p0.05). (2) The extract of Astragalus extract increased the exercise of large-intensity endurance. The level of IGF-1 and the level of IGF-1R in the gastrocnemius of the rat and the quiet control There was no significant difference in the level of IGF-1 in the rats in the exercise group compared with the group. In addition, the content of IGF-1R in the gastrocnemius was not significantly different from that of the quiet group, and the level of IGF-1R in the control group was compared with that of the exercise group. The expression of IGF-1R in gastrocnemius was significantly higher (p0.05). (3) The expression of IGF-1R in gastrocnemius was significantly higher (p0.05). (3) The expression of IGF-1R in gastrocnem@@ Compared with the control group, the total protein concentration of the gastrocnemius muscle and the protein level of MHC in the rats with high-intensity endurance exercise were higher than that of the control group. The relative weight of the fish muscle and the gastrocnemius was significantly increased, and the protein concentration of the gastrocnemius was also increased. The relative weight and protein concentration of the soleus muscle and the gastrocnemius muscle in the rats were not significantly different from those in the exercise group, but the relative weight of the gastrocnemius and the protein concentration of the soleus muscle were not increased. There was a significant difference, but the relative weight of the soleus was reduced unexpectedly (p0.05). 5). In addition, there was no significant difference in the level of MHC protein in the exercise group compared to the quiet group. Compared with the exercise group, the level of MHC protein was significantly higher in the + exercise group (p0.05). (4) The level of MHC protein in the exercise group was significantly higher than that of the exercise group (p0.05). After one-time exhaustive exercise of the high-intensity endurance exercise and the non-moving rats The blood glucose level of 0 min can decrease the blood glucose level before the exercise and the non-moving rats. The blood glucose level is compared with the quiet group, and the blood sugar level is significantly increased at 30 min after the exhaustive exercise. The blood glucose levels in each group were not significant after 2 h after exercise, respectively (p0.05, p0.01). The difference. (5) The amount of Hb and red blood cells in the high-strength endurance exercise and the non-moving rats were not affected by the extract of the extract. Conclusion:1) The mechanism of enhancing the exercise ability of the rats with high-intensity endurance exercise may increase the level of testosterone/ AR and the level of IGF-1/ IGF-1R. the protein concentration of the gastrocnemius muscle, the level of the MHC protein,
【学位授予单位】：上海体育学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：G804.2

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