亮氨酸改善盲肠结扎穿孔小鼠生长激素抵抗

发布时间：2019-06-21 01:08

【摘要】：目的:探讨盲肠结扎穿孔小鼠生长激素-胰岛素样生长因子-1(GH-IGF-1)轴的变化及亮氨酸的干预作用。方法:将40只小鼠随机分为四组,每组10只。即正常对照组(Control组)、假手术组(Sham组)、盲肠结扎穿孔等渗盐水组(CLP-N组)和盲肠结扎穿孔亮氨酸组(CLP-L组)。采用盲肠结扎穿孔法制作腹腔感染模型后,CLP-N组给予等渗盐水灌胃,CLP-L组给予亮氨酸灌胃,连续7 d。第8天腹腔麻醉后取门静脉血,检测小鼠血清生长激素(GH)和胰岛素样生长因子-1(IGF-1)水平。同时测定小鼠肝组织生长激素受体(GHR)、IGF-1、胰岛素样生长因子结合蛋白-1(IGFBP-1)和胰岛素样生长因子结合蛋白-3(IGFBP-3)mRNA表达量。结果:与Control组比,CLP-N组小鼠血清GH水平明显升高(P0.01)、而血清IGF-1水平急剧下降(P0.01),并且伴随着肝组织GHR和IGFBP-3 mRNA水平显著降低(P0.01)以及IGFBP-1mRNA水平明显升高(P0.01);而CLP-L组小鼠血清GH水平明显升高(P0.01)、血清IGF-1水平轻微下降(P0.05)以及肝组织GHR、IGFBP-1和IGFBP-3 mRNA水平明显升高(P0.01)。结论:盲肠结扎穿孔小鼠存在获得性GH抵抗,亮氨酸可改善此现象。
[Abstract]:Aim: to investigate the changes of growth hormone-insulin-like growth factor-1 (GH-IGF-1) axis in mice with cecal ligation and perforation and the intervention effect of Leucine. Methods: forty mice were randomly divided into four groups with 10 mice in each group. That is, normal control group (Control group), pseudo-operation group (Sham group), cecal ligation and perforation isoosmotic saline group (CLP-N group) and cecal ligation and perforation Leucine group (CLP-L group). After abdominal infection model was established by cecal ligation and perforation, CLP-N group was given isotonic saline and CLP-L group was given Leucine intragastrically for 7 days. On the 8th day after intraabdominal anesthesia, portal vein blood was taken to detect the levels of serum growth hormone (GH) and insulin-like growth factor-1 (IGF-1) in mice. The expression of growth hormone receptor (GHR), IGF-1, insulin-like growth factor binding protein-1 (IGFBP-1) and insulin-like growth factor binding protein-3 (IGFBP-3) mRNA in mouse liver tissue was also measured. Results: compared with Control group, the level of serum GH in CLP-N group was significantly higher than that in CLP-N group (P01), while the level of serum IGF-1 decreased sharply (P01), accompanied by the decrease of GHR and IGFBP-3 mRNA levels in liver tissue (P01) and the increase of IGFBP-1mRNA level (P01). In CLP-L group, the serum GH level was significantly increased (P01), the serum IGF-1 level was slightly decreased (P05), and the GHR,IGFBP-1 and IGFBP-3 mRNA levels in liver tissue were significantly increased (P01). Conclusion: acquired GH resistance exists in mice with cecal ligation and perforation, and Leucine can improve this phenomenon.
【作者单位】：南方医科大学;南京军区南京总医院解放军普通外科研究所;
【基金】：国家自然科学基金资助项目(81470797)
【分类号】：R459.3

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