薯蓣丸加减方治疗变应性鼻炎大鼠的实验研究
发布时间：2019-06-20 23:25
【摘要】：目的：复制变应性鼻炎(AR)大鼠模型，观察薯蓣丸加减方对AR大鼠行为学指标、血清总IgE和IL-4含量值、鼻黏膜病理形态及水通道蛋白5(AQP5)表达的影响，初步探讨该方治疗AR的作用机理，为该方更好地运用于临床提供实验理论依据。 方法：SPF级SD大鼠70只，雄性，随机分为：正常对照组、模型组、辛芩颗粒组、通窍鼻炎片组及薯蓣丸加减方高、中、低剂量组，每组10只。除正常对照组外，各组采用卵白蛋白(OVA)复制AR大鼠模型。造模成功后，模型组和正常对照组给予口饲生理盐水(1ml/0.1kg·d)。高、中、低剂量组分别给予薯蓣丸加减方水煎剂(32、16、8g/kg·d)灌胃，阳性对照组采用辛芩颗粒(5g/kg·d)、通窍鼻炎片(1.8g/kg·d)灌胃。均给药14d后，麻醉大鼠，抽取腹主动脉血，用酶联免疫吸附测定法(ELISA)检测血清总IgE、IL-4含量值；处死大鼠摘取鼻黏膜，，HE染色观察；免疫组化SABC法检测鼻黏膜AQP5表达。 结果：造模后各组行为学积分均高于正常对照组(P＜0.01)；各组间差异不明显(P＞0.05)；灌胃14d后，各治疗组行为学积分均低于模型组(P＜0.01)。模型组血清总IgE、IL-4含量均高于正常对照组(P＜0.01)；各治疗组均低于模型组(P＜0.01)；高、低剂量组与辛芩颗粒组比较，差异不明显(P＞0.05)；中剂量组较辛芩颗粒组降低明显(P＜0.05)；高、中、低剂量组与通窍鼻炎片组比较，差异无统计学意义(P＞0.05)。光镜下，模型组大鼠鼻黏膜以大量炎细胞浸润、水肿、充血，腺体增生为主，部分上皮组织脱落。各治疗组鼻黏膜病理形态较模型组有显著改善，以中剂量组对组织的修复作用突出。模型组AQP5平均光密度值(MOD)低于正常对照组(P＜0.05)；除辛芩颗粒组外，各治疗组均高于模型组(P＜0.05)；高、中剂量组高于通窍鼻炎片组(P＜0.01)；低剂量组与通窍鼻炎片组比较，差异无统计学意义(P＞0.05)；高、中剂量组高于低剂量组(P＜0.01)；而高、中剂量组间比较，无统计学意义(P＞0.05)。结论：薯蓣丸加减方对AR大鼠疗效显著。降低血清IL-4含量，抑制IgE合成，减轻 免疫炎症反应，缓解鼻黏膜组织炎症细胞浸润，促进AQP5蛋白表达，调节鼻腔水液代谢，改善喷嚏、鼻痒、流涕等临床症状，可能是该方对AR良好疗效的作用机理之一。
[Abstract]:Aim: to establish the (AR) rat model of allergic rhinitis, to observe the effects of Dioscozhuang Pill on behavioral indexes, serum total IgE and IL-4 content, nasal mucosa pathological morphology and aquaporin 5 (AQP5) expression in AR rats, and to explore the mechanism of the prescription in the treatment of AR, so as to provide experimental theoretical basis for its better application in clinical practice. Methods: 70 SPF SD rats, male, were randomly divided into normal control group, model group, Xinqin granule group, Tongqiao rhinitis tablet group and Dioscorea zingiberensis pill addition and subtraction prescription group with 10 rats in each group. In addition to the normal control group, the AR rat model was established by ovalbumin (OVA) in each group. After the successful establishment of the model, the model group and the normal control group were given oral saline (1ml/ 0.1kg 路d). The high, middle and low dose groups were given Dioscorea Pill decoction (32, 16, 8 g / kg 路d), while the positive control group was given Xinqin granule (5g/ kg 路d), Tongqiao Biyan tablet (1.8g/ kg 路d). After 14 days of administration, abdominal aortic blood was taken from anesthetized rats, the total IgE,IL-4 content in serum was detected by enzyme-linked immunosorbent assay (ELISA), the nasal mucosa was removed and observed by HE staining, and the expression of AQP5 in nasal mucosa was detected by SABC method. Results: after modeling, the behavioral scores of each group were higher than those of the normal control group (P < 0.01), but there was no significant difference among the three groups (P > 0.05). After 14 days of intragastric administration, the behavioral scores of each treatment group were lower than those of the model group (P < 0.01). The content of serum total IgE,IL-4 in the model group was higher than that in the normal control group, and that in each treatment group was lower than that in the model group (P < 0.01). There was no significant difference between the high and low dose groups and Xinqin granule group (P > 0.05), but there was no significant difference between the high, middle and low dose groups and Tongqiao rhinitis tablet group (P > 0.05). Under light microscope, a large number of inflammatory cells infiltrated, edema, hyperemia, gland hyperplasia and partial epithelial tissue shedding in the nasal mucosa of the model group. The pathological morphology of nasal mucosa in each treatment group was significantly better than that in the model group, and the repair effect of the middle dose group on the tissue was more prominent. The mean optical density of AQP5 in the model group was lower than that in the normal control group (P < 0.05), except Xinqin granule group, all the treatment groups were higher than the model group (P < 0.05), the high and middle dose groups were higher than those in the Tongqiao rhinitis tablet group (P < 0.01), and there was no significant difference between the low dose group and the Tongqiao rhinitis tablet group (P > 0.05), and the high and middle dose groups were higher than the low dose group (P < 0.01). There was no significant difference between high and middle dose groups (P > 0.05). Conclusion: Dioscorea Pill is effective in AR rats. Reducing serum IL-4 content, inhibiting IgE synthesis, reducing immune inflammatory reaction, alleviating inflammatory cell infiltration in nasal mucosa, promoting AQP5 protein expression, regulating nasal water metabolism, improving sneezing, nasal itching, runny nose and other clinical symptoms may be one of the good therapeutic effects of this prescription on AR.
【学位授予单位】：云南中医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R276.1
[Abstract]:Aim: to establish the (AR) rat model of allergic rhinitis, to observe the effects of Dioscozhuang Pill on behavioral indexes, serum total IgE and IL-4 content, nasal mucosa pathological morphology and aquaporin 5 (AQP5) expression in AR rats, and to explore the mechanism of the prescription in the treatment of AR, so as to provide experimental theoretical basis for its better application in clinical practice. Methods: 70 SPF SD rats, male, were randomly divided into normal control group, model group, Xinqin granule group, Tongqiao rhinitis tablet group and Dioscorea zingiberensis pill addition and subtraction prescription group with 10 rats in each group. In addition to the normal control group, the AR rat model was established by ovalbumin (OVA) in each group. After the successful establishment of the model, the model group and the normal control group were given oral saline (1ml/ 0.1kg 路d). The high, middle and low dose groups were given Dioscorea Pill decoction (32, 16, 8 g / kg 路d), while the positive control group was given Xinqin granule (5g/ kg 路d), Tongqiao Biyan tablet (1.8g/ kg 路d). After 14 days of administration, abdominal aortic blood was taken from anesthetized rats, the total IgE,IL-4 content in serum was detected by enzyme-linked immunosorbent assay (ELISA), the nasal mucosa was removed and observed by HE staining, and the expression of AQP5 in nasal mucosa was detected by SABC method. Results: after modeling, the behavioral scores of each group were higher than those of the normal control group (P < 0.01), but there was no significant difference among the three groups (P > 0.05). After 14 days of intragastric administration, the behavioral scores of each treatment group were lower than those of the model group (P < 0.01). The content of serum total IgE,IL-4 in the model group was higher than that in the normal control group, and that in each treatment group was lower than that in the model group (P < 0.01). There was no significant difference between the high and low dose groups and Xinqin granule group (P > 0.05), but there was no significant difference between the high, middle and low dose groups and Tongqiao rhinitis tablet group (P > 0.05). Under light microscope, a large number of inflammatory cells infiltrated, edema, hyperemia, gland hyperplasia and partial epithelial tissue shedding in the nasal mucosa of the model group. The pathological morphology of nasal mucosa in each treatment group was significantly better than that in the model group, and the repair effect of the middle dose group on the tissue was more prominent. The mean optical density of AQP5 in the model group was lower than that in the normal control group (P < 0.05), except Xinqin granule group, all the treatment groups were higher than the model group (P < 0.05), the high and middle dose groups were higher than those in the Tongqiao rhinitis tablet group (P < 0.01), and there was no significant difference between the low dose group and the Tongqiao rhinitis tablet group (P > 0.05), and the high and middle dose groups were higher than the low dose group (P < 0.01). There was no significant difference between high and middle dose groups (P > 0.05). Conclusion: Dioscorea Pill is effective in AR rats. Reducing serum IL-4 content, inhibiting IgE synthesis, reducing immune inflammatory reaction, alleviating inflammatory cell infiltration in nasal mucosa, promoting AQP5 protein expression, regulating nasal water metabolism, improving sneezing, nasal itching, runny nose and other clinical symptoms may be one of the good therapeutic effects of this prescription on AR.
【学位授予单位】：云南中医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R276.1
【参考文献】
相关期刊论文 前10条
1 张罗,周兵,韩德民,顾之燕;变应性鼻炎研究进展(一):发病机制[J];耳鼻咽喉头颈外科;2003年05期
2 张罗;顾之燕;郑铭;韩德民;;过敏性鼻炎患病状况研究[J];中国耳鼻咽喉头颈外科;2009年11期
3 高学清,雷福云;薯蓣糖浆防治小儿反复呼吸道感染39例[J];国医论坛;2001年02期
4 崔秀丽;许振林;;顽固性皮肤瘙痒从脾论治验案3则[J];中医药临床杂志;2009年04期
5 李寿v
本文编号：2503599
本文链接：https://www.wllwen.com/yixuelunwen/mazuiyixuelunwen/2503599.html
最近更新
教材专著
