尿路感染分离金黄色葡萄球菌的耐药性研究

发布时间：2018-02-13 08:48

本文关键词： 金黄色葡萄球菌耐药基因耐药性 K-B法　出处：《中国病原生物学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的研究金黄色葡萄球菌的耐药情况及耐药基因检出情况,为菌株感染的防治提供指导。方法收集尿路感染者送检标本,进行金黄色葡萄球菌培养及分离纯化,鉴定菌株,并采用PCR检测耐药基因。结果共分离金黄色葡萄球菌174株,K-B法测定金黄色葡萄球菌对阿米卡星、苯唑西林、四环素、红霉素、万古霉素、环丙沙星、替考拉宁、克林霉素、利福平、庆大霉素的耐药率分别为14.94%、39.08%、37.93%、59.20%、0、31.03%、0、47.70%、17.82%和45.98%,中介率分别为10.92%、0、5.17%、1.72%、0、2.87%、0、9.20%、0和6.90%,敏感率分别为74.14%、60.92%、56.90%、39.08%、100.00%、66.09%、100.00%、43.10%、82.18%和47.13%。PCR扩增金黄色葡萄球菌ermA基因为421bp、ermC基因为572bp、qacA基因为629bp、ermB基因为359bp、aac(6')/aph(2')基因为220bp、mecA基因为162bp,检出率分别为21.26%、10.92%、16.09%、35.06%、29.89%和43.10%。结论分离自尿路感染者尿液的金黄色葡萄球菌对常用抗生素有一定耐药性,在抗感染治疗中可优先考虑使用万古霉素和替考拉宁,但仍应合理选用。为控制菌株耐药性发生及发展,应及时分析菌株耐药基因分布情况。
[Abstract]:Objective to study the drug resistance of Staphylococcus aureus and the detection of drug resistance gene, and to provide guidance for the prevention and treatment of Staphylococcus aureus infection. Methods the samples of urinary tract infections were collected, the strains were cultured, isolated and purified, and the strains were identified. Results A total of 174 strains of Staphylococcus aureus were isolated for the determination of amikacin, oxacillin, tetracycline, erythromycin, vancomycin, ciprofloxacin, teicoplanin, tetracycline, tetracycline and clindamycin. Rifampicin, The drug resistance rates of gentamicin were 14.942% and 45.98%, respectively, and the intermediate rates were 10.922% and 1.722mC, respectively. The sensitive rates were 74.1460.9228.56.90 and 39.080.100.0066.09100.0066.09100.0066.09100.0043.10100.0043.10100.0042.18% and 47.13.PCR, respectively. Conclusion Staphylococcus aureus isolated from urine of patients with urinary tract infection is resistant to common antibiotics, which is 29.89% and 43.10% respectively. Vancomycin and teicoplanin should be given priority in anti-infective treatment, but should be reasonably selected. In order to control the occurrence and development of drug resistance of strains, the distribution of drug resistance genes of strains should be analyzed in time.
【作者单位】：郑州大学第二附属医院肾病风湿科;
【分类号】：R691.3

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