沉默CHD1L对前列腺癌PC3细胞恶性生物学行为的影响及其可能机制

发布时间：2018-03-11 12:55

本文选题：前列腺癌　切入点：染色质解旋酶DNA结合蛋白样基因　出处：《中国肿瘤生物治疗杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的:探讨染色质解旋酶DNA结合蛋白1样基因(chromodomain helicase/ATPase DNA binding protein 1-like gene,CHD1L)对前列腺癌细胞侵袭、迁移能力的影响及其可能的作用机制。方法:采用实时荧光定量PCR技术检测前列腺癌细胞株LNCAP、PC3、DU145以及前列腺上皮细胞株RWPE-1中CHD1L mRNA表达水平;转染siRNA干扰前列腺癌PC3细胞CHD1L的表达,并用Transwell侵袭实验和划痕实验分析沉默CHD1L对前列腺癌细胞侵袭和迁移能力的影响;Western blotting检测PC3细胞MMP-9、N-钙黏蛋白和E-钙黏蛋白的表达水平。结果:CHD1L mRNA在前列腺癌细胞中的表达水平明显高于前列腺上皮细胞(P0.01),其中以前列腺癌PC3细胞的表达水平最高。侵袭实验中,干扰组的穿膜细胞数明显低于阴性对照组和空白对照组[(49.67±6.67)vs(113.67±5.69)和(112.00±12.49)个,P0.05)。划痕实验中,干扰组48 h伤口愈合率也低于阴性对照组和空白对照组[(21.27±3.27)%vs(48.47±5.72)%和(49.93±3.35)%,P0.05]。干扰组细胞MMP-9和N-钙黏蛋白表达下调,E-钙黏蛋白表达上调。结论:沉默CHD1L可降低前列腺癌PC3细胞的侵袭迁移能力,该作用可能是通过调控MMP-9和EMT相关蛋白表达实现的。
[Abstract]:Objective: to investigate the invasion of chromatin helicase DNA binding protein-1 gene (helicase/ATPase DNA binding protein 1-like gene CHD1L) on prostate cancer cells. Methods: the expression of CHD1L mRNA in prostate cancer cell line LNCAP- PC3DU145 and prostatic epithelial cell line RWPE-1 was detected by real-time fluorescence quantitative PCR, and the expression of CHD1L in PC3 cell line was interfered with by transfection of siRNA. The effect of silencing CHD1L on the invasion and migration of prostate cancer cells was analyzed by Transwell invasion assay and scratch test. The expression of MMP-9 N- cadherin and Ecadherin in PC3 cells was detected by Western blotting. The expression level of PC3 cells in prostate cancer was significantly higher than that in prostatic epithelial cells (P0.01), and the highest expression level was found in prostate cancer PC3 cells. The number of perforating cells in the interference group was significantly lower than that in the negative control group and the blank control group [49.67 卤6.67 vs 113.67 卤5.69] and 112.00 卤12.49, respectively. The wound healing rate in the interference group was also lower than that in the negative control group and the blank control group [21.27 卤3.27% vs 48.47 卤5.72% and 49.93 卤3.35% respectively]. The expression of MMP-9 and N- cadherin in the interfering group was down-regulated. Conclusion: silencing CHD1L can reduce the invasion and migration ability of prostate cancer PC3 cells. This effect may be achieved by regulating the expression of MMP-9 and EMT related proteins.
【作者单位】：河南省人民医院检验科;郑州大学附属肿瘤医院病理科;
【基金】：河南省医学科技攻关计划项目(No.201303155)~~
【分类号】：R730.43;R737.25

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