microRNA-214在膀胱癌中表达的生物学功能、调控机制及临床应用探讨

发布时间：2018-03-18 19:39

本文选题：microRNA-　切入点：膀胱癌　出处：《中华肿瘤防治杂志》2016年S1期 　论文类型：期刊论文

【摘要】：目的分析microRNA-214在膀胱癌中表达的生物学功能、调控机制以及临床应用探讨。方法选取我院2014-2015年收治的膀胱癌120例患者作为研究对象,设为实验组,取120例患者的膀胱癌新鲜冰冻组织,其中包括40例NMIBC,80例MIBC,选取120例正常人作为对照组。采用qRT-PCR方法对两组患者中的生化上皮细胞以及两种膀胱癌细胞系中的表达情况,采用体外用脂体2 000将miR-214模拟物进行转染至膀胱癌细胞中,设计阴性对照组和空白对照组。采用qRT-PCR方法评估各组转染效率。利用综合生物学信息分析平台,联合五种计算方法,寻找miR-214的靶基因,并且采用双荧光素酶报告系统进行验证。采用siRNA对膀胱癌细胞系中的靶基因的表达水平进行干扰。结果miR-214在两种膀胱癌细胞中的表达与生化上皮细胞比较明显下降,差异有统计学意义,P0.05。为患者进行随访发现,120例患者中有66例患者膀胱癌复发,复发率为55%。总生存患者有72例,总生存率为60%。结论 miR-214分析在膀胱癌组织中出现表达水平下降,与膀胱癌负面性临床病例特征有密切联系,提示膀胱癌疾病的发生和发展,在其过程中发挥重要的作用。MiR-214在膀胱癌疾病中发挥抑制癌基因的作用,抑制膀胱癌细胞的增值和迁移,促进癌细胞凋亡。
[Abstract]:Objective to analyze the biological function, regulatory mechanism and clinical application of microRNA-214 expression in bladder cancer. Methods 120 patients with bladder cancer admitted in our hospital from 2014 to 2015 were selected as the experimental group. The fresh frozen tissues of 120 patients with bladder cancer, including 40 patients with NMIBC, 80 patients with MIBCs, and 120 normal controls were selected as control group. The expression of biochemical epithelial cells and two kinds of bladder cancer cell lines were detected by qRT-PCR method. MiR-214 mimics were transfected into bladder cancer cells with lipofectin 2 000 in vitro. Negative control group and blank control group were designed. Transfection efficiency of each group was evaluated by qRT-PCR method. Looking for the target gene of miR-214, The expression level of target gene in bladder cancer cell line was interfered by siRNA. Results the expression of miR-214 in two kinds of bladder cancer cell lines was significantly lower than that in biochemical epithelial cells. The difference was statistically significant (P 0.05). 66 out of 120 patients with bladder cancer recurred, and the recurrence rate was 555.The total survival rate was 60% and 72 patients survived. Conclusion the expression level of miR-214 in bladder cancer tissues decreased. It is suggested that the occurrence and development of bladder cancer play an important role in the development of bladder cancer. MiR-214 plays an important role in the suppression of oncogene in bladder cancer. Inhibit the proliferation and migration of bladder cancer cells, promote apoptosis of cancer cells.
【作者单位】：郑州大学第二附属医院;
【分类号】：R737.14

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