肾动脉移植骨髓间充质干细胞治疗大鼠慢性肾病结果分析

发布时间：2018-03-28 14:34

本文选题：骨髓间充质干细胞　切入点：干细胞移植　出处：《介入放射学杂志》2017年04期

【摘要】：目的分析和评价经肾动脉途径移植骨髓间充质干细胞(BMSC)对慢性阿霉素肾病模型大鼠的治疗结果。方法采用50只雄性Sprague-Dawley大鼠作为实验动物。其中2只12周龄大鼠用于BMSC分离培养;12只作为空白对照组(N组),36只经尾静脉注入阿霉素制作慢性肾病大鼠模型,并随机分为慢性肾病对照组(C组)、经肾动脉移植BMSC组(A组)和经尾静脉移植BMSC组(V组),每组12只。N组经尾静脉注入等量0.9%氯化钠溶液。结果 A组、V组、C组在各观察时点血尿素氮、血肌酐、24 h尿蛋白、24 h尿微量蛋白水平均明显高于N组(P0.01)。移植后1、2周A组24 h尿微量蛋白均低于C组(P0.01),且血肌酐均较C组和V组低(P0.01);移植后1周A组24 h尿蛋白、24 h尿微量蛋白均明显低于V组(P0.01),但2周时与V组差异无统计学意义。结论经肾动脉途径移植BMSC可提高细胞归巢效率,并提高对损伤组织的修复作用。
[Abstract]:Objective to analyze and evaluate the therapeutic effect of bone marrow mesenchymal stem cells (MSCs) transplantation via renal artery in chronic adriamycin nephropathy rats. Methods 50 male Sprague-Dawley rats were used as experimental animals. Twelve rats were isolated and cultured in BMSC as control group. 36 rats in group N were injected adriamycin into caudal vein to make chronic nephropathy model. They were randomly divided into chronic nephropathy control group (C group), renal artery transplantation group (BMSC group A group A) and tail vein transplantation BMSC group (group V). 12 rats in each group were injected with 0.9% sodium chloride solution via tail vein. Observe the blood urea nitrogen, The levels of 24 h urinary trace protein in group A were significantly lower than those in group C at 2 weeks after transplantation, and the levels of serum creatinine in group A were lower than those in group C and group V at 24 h, and in group A at 1 week after transplantation, the levels of urinary trace protein in group A were lower than those in group C and group V, and in group A at 24 h after transplantation, the levels of serum creatinine in group A were lower than those in group C and group V at 24 h after transplantation. Urinary microproteins in 24 h were significantly lower than those in group V (P 0.01), but there was no significant difference between group V and group 2 at 2 weeks. Conclusion Transrenal artery transplantation of BMSC can improve the efficiency of homing. And improve the repair effect of injured tissue.
【作者单位】：昆明医科大学第二附属医院放射科;
【分类号】：R699.2

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