miRNA-93在膀胱癌中的表达及其对T24细胞生物学特性的影响

发布时间：2018-04-09 16:06

本文选题：miRNA-　切入点：膀胱癌　出处：《中国肿瘤生物治疗杂志》2017年02期

【摘要】：目的:探讨miRNA-93在膀胱癌中的表达及对人膀胱癌细胞株T24细胞生物学特性的影响及其作用机制。方法:选取郑州大学附属南阳市中心医院泌尿外科2010年5月至2016年5月收治的79例膀胱癌患者病理组织标本及配对癌旁正常组织,通过qRT-PCR检测miRNA-93的表达水平。沉默miRNA-93后,采用CCK-8法、Transwell实验和流式细胞术分别检测细胞增殖和迁移能力的变化及细胞的凋亡情况;Western blotting检测AKT/p-AKT、GSK3β/p-GSK3β蛋白表达水平的变化。结果:miRNA-93在膀胱癌组织及癌细胞中高表达,且表达水平与癌灶大小、淋巴结转移、病理分级及T分期有关(均P0.05)。沉默miRNA-93后,T24细胞的增殖能力显著降低(P0.05),沉默miRNA-93促进T24细胞的凋亡并抑制其迁移;同时,沉默miRNA-93后p-AKT和p-GSK3β的蛋白表达水平均显著下降(P0.05)。结论:miRNA-93能够促进人膀胱癌细胞株T24细胞的增殖和迁移,并抑制凋亡,其作用机制可能与下调p-AKT、p-GSK3β蛋白表达有关,提示miRNA-93可以作为诊断和靶向治疗膀胱癌的潜在作用位点。
[Abstract]:Aim: to investigate the expression of miRNA-93 in bladder cancer and its effect on the biological characteristics of human bladder cancer cell line T24 and its mechanism.Methods: qRT-PCR was used to detect the expression of miRNA-93 in 79 specimens of bladder cancer from May 2010 to May 2016 in the Department of Urology, Nanyang Central Hospital affiliated to Zhengzhou University.After silencing miRNA-93, the changes of cell proliferation and migration ability and apoptosis were detected by CCK-8 assay and flow cytometry respectively. Western blotting was used to detect the expression of GSK3 尾 / p-GSK3 尾 protein in AKT / p-AKTU GSK3 尾 / p-GSK3 尾.Results the expression of microRNA-93 was highly expressed in bladder cancer tissues and cancer cells, and the expression level was related to the size of tumor focus, lymph node metastasis, pathological grade and T stage (all P 0.05).After silencing miRNA-93, the proliferative ability of T24 cells decreased significantly, and the apoptosis and migration of T24 cells were inhibited by silencing miRNA-93, while the protein expression of p-AKT and p-GSK3 尾 decreased significantly after miRNA-93 silencing.Conclusion: 1 miRNA-93 can promote the proliferation and migration of human bladder cancer cell line T24 and inhibit apoptosis. The mechanism may be related to the down-regulation of p-AKTc-p-GSK3 尾 protein, suggesting that miRNA-93 may be a potential site for the diagnosis and targeted treatment of bladder cancer.
【作者单位】：郑州大学附属南阳市中心医院肾病风湿免疫科;郑州大学附属南阳市中心医院骨二科;郑州大学附属南阳市中心医院泌尿外科;郑州大学第一附属医院泌尿外科;
【基金】：河南省基础与前沿技术研究计划基金资助(No.142300410034)~~
【分类号】：R737.14

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