热毒炽盛型和阴虚内热型狼疮性肾炎与调节性T细胞、干扰素-α的关系探讨

发布时间：2018-04-24 11:59

本文选题：狼疮性肾炎 + 调节性T淋巴细胞　；参考：《福建中医药大学》2014年硕士论文

【摘要】：目的：通过检测热毒炽盛型和阴虚内热型狼疮性肾炎患者外周血调节性T细胞、IL-10、IL-6、干扰素-α水平,探讨微观指标与中医证型及疾病活动性之间的关系,寻求狼疮性肾炎中医辨证的物质基础,为探索中医证型的本质提供实验证据。方法：收集符合纳入标准的热毒炽盛型狼疮性肾炎患者28例,阴虚内热型狼疮性肾炎患者27例,健康对照组21例,应用三联荧光染色通过流式细胞仪分析外周血中Treg细胞水平,并用CBA流式蛋白分析系统检测外周血中IL-10、IL-6、IFN-α水平。结果： 1、热毒炽盛组IL-10、IL-6水平明显高于阴虚内热组(P0.01)；热毒炽盛组Treg显著低于阴虚内热组和正常组(P0.01),阴虚内热组和正常组比较Treg有显著性差异(P0.05)；热毒炽盛组IFN-α显著高于阴虚内热组和正常组(P0.01),阴虚内热组和正常组比较IFN-α无显著性差异(P0.05)。 2、狼疮性肾炎患者外周血Treg与SLEDAI、IL-10、IL-6呈负相关(P0.05)。狼疮性肾炎患者外周血IFN-α与SLEDAI、IL-10、IL-6呈正相关(P0.01)。Treg与IFN-α之间无相关性。结论： 1、LN患者外周血Treg水平与正常组比较有显著性差异,Treg减少可能是狼疮性肾炎患者出现免疫功能异常的重要原因。 2、热毒炽盛组LN患者Treg水平与阴虚内热组比较有显著性差异,可作为区别两证型的微观指标。 3、热毒炽盛组LN患者IFN-α水平与阴虚内热组比较有显著性差异,可作为区别两证型的微观指标。 4、狼疮性肾炎患者外周血Treg与SLEDAI、IL-10、IL-6呈负相关,IFN-α与SLEDAI、 IL-10、IL-6呈正相关。因此IFN-α、IL-10、IL-6、Treg可作为狼疮活动性的指标。但IFN-α和Treg之间并没有相关性。
[Abstract]:Objective: to investigate the relationship between microcosmic indexes and TCM syndromes and disease activity by detecting the levels of IL-10 / IL-6 and IFN- 伪 in peripheral blood regulatory T cells in patients with lupus nephritis with excessive heat toxin and internal heat of yin deficiency. To seek the material basis of TCM syndrome differentiation of lupus nephritis, to provide experimental evidence for exploring the essence of TCM syndrome type. Methods: Twenty-eight patients with heat toxic and incandescent lupus nephritis, 27 patients with heat type lupus nephritis due to yin deficiency and 21 healthy controls were collected. The levels of Treg cells in peripheral blood were analyzed by flow cytometry with triple fluorescent staining. The level of IL-6 and IFN-伪 in peripheral blood was detected by CBA flow protein analysis system. Results: 1. The level of IL-6 in heat toxic heat group was significantly higher than that in yin deficiency group (P 0.01), the Treg level in heat toxin heat group was significantly lower than that in yin deficiency group and normal group (P 0.01), there was significant difference in Treg between yin deficiency heat group and normal group (P 0.05), and the level of IFN- 伪 in heat toxin heat group was significantly higher than that in yin deficiency group. There was no significant difference in IFN- 伪 between the internal heat group and the normal group. 2. There was a negative correlation between Treg and IL-6 in patients with lupus nephritis. There was no positive correlation between IFN- 伪 and IL-6 in peripheral blood of patients with lupus nephritis. Conclusion: 1There was significant difference in the level of Treg in peripheral blood between LN patients and normal controls. The decrease of Treg may be an important reason for abnormal immune function in patients with lupus nephritis. 2. The level of Treg in LN group was significantly different from that in Yin deficiency group, and could be used as a microscopic index to distinguish the two syndromes. 3. The level of IFN- 伪 in LN group was significantly different from that in Yin-deficiency group, and could be used as a microscopic index to distinguish the two syndrome types. 4. There was a negative correlation between Treg and IL-6 in peripheral blood of patients with lupus nephritis and a positive correlation between IFN- 伪 and SLEDAI, IL-10- 6 in patients with lupus nephritis. Therefore, IFN- 伪 IL-10 and IL-6 Treg may be used as an indicator of lupus activity. But there is no correlation between IFN- 伪 and Treg.
【学位授予单位】：福建中医药大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R593.242

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