昆明地区110例精液检测结果分析及健康人与特发性弱精子症患者精浆蛋白组学比较的初步研究

发布时间：2018-05-02 05:53

本文选题：特发性弱精子症 + 精液分析　；参考：《昆明医科大学》2017年硕士论文

【摘要】：[目的]通过对110例精液检验结果的分析了解昆明市男性不育的主要影响因素,并对特发性弱精子症患者与精液分析结果正常人群的精浆蛋白组化检测出两组人群中精浆蛋白质水平,以初步探讨蛋白质在两组人群中的差异。[方法]通过计算机辅助精液分析系统(CASA)得出分析结果并做数据分析,其中选取较典型的10名符合弱精子症诊断标准的育龄期男性作为特发性弱精子症组,8名年龄相匹配的健康者作为对照组。采集特发性弱精子症组和对照组所有受试对象的精浆冻干粉作为标本。应用LC-MS质谱鉴定(Shotgun)法检测所有标本中蛋白质的表达水平。所有结果均利用统计软件SPSS进行分析,以P0.05说明有统计学意义。[结果]1、110例患者精液标本中,精液量1.5ml的有16例,占14. 55%;酸碱度7. 2的有1例,占0.91%;精子浓度小于15×106的有36例,占32. 73%;精子总数小于39X 106的有43例,占39. 09%;精子总活力(PR+NP) 40%的有82例,占74. 55%;向前运动力(PR)小于32%的有84例,占76. 36%;液化时间60min的有3例,占2.73% ;畸形精子96%的有3例,占2. 73%。2、特发性弱精症患者的蛋白质组化结果共有274个蛋白质点与健康组不同(参考水平在三倍以上为有意义),差异均有统计学意义(P0.05)。3、在不同的274个不同蛋白质中,共有109个蛋白质点在特发性弱精症患者精浆中的含量大于健康组含量,差异均有统计学意义(P0.05),其中,99个蛋白质点在健康组中未表达。4、在不同的274个不同蛋白质中,共有165个蛋白质点在特发性弱精症患者精浆中的含量小于健康组含量,均有统计学意义,(P0.05),其中,144个蛋白质点在特发性弱精症患者精浆中未表达。5、以上我们排除了较小蛋白质片段与未经鉴定的蛋白质,其中热休克蛋白、角蛋白、附睾分泌蛋白、Ras蛋白、组蛋白、前列腺干细胞抗原较有意义,并讨论论证了它们可能均通过不同的机制影响到弱精子症的发生、发展的病理过程。[结论]在精液常规各项目异常指标中,昆明地区弱精子症患者的精液以精子活力异常多见,而且精子活力异常往往同时伴有精子总数或者精子浓度的异常。精浆中的多种蛋白质参与了弱精子症发生、发展的病理过程,对判断患者疾病的严重程度、预后及疗效有一定意义,且可以作出初步假设,各种蛋白质在弱精子症的发生发展中起到不同的作用,为下一步研究奠定了基础。
[Abstract]:[objective] to understand the main influencing factors of male infertility in Kunming by analyzing the results of 110 semen tests. The seminal plasma protein levels of the two groups were detected by the seminal plasma protein histochemistry in order to explore the difference of the proteins in the two groups. [methods] the results were obtained by computer aided semen analysis system (CASAA) and the data were analyzed. Among them, 10 typical infertile men who met the diagnostic criteria of asthenospermia were selected as the control group with 8 age-matched healthy persons as the idiopathic asthenospermia group. The seminal plasma freeze-dried powder of all subjects in idiopathic asthenospermia group and control group were collected as specimens. LC-MS mass spectrometry was used to detect the protein expression in all specimens. All the results were analyzed by statistical software SPSS. [results] of the 1110 semen samples, 16 (14.4%) had 1.5ml. The alkalinity is 7. 2 in 1 case (0. 91%) and sperm concentration less than 15 脳 10 6 in 36 cases (32. 2%). The total number of spermatozoa was less than 39X106 in 43 cases (39. 5%). The percentage of total sperm motility PR NPs was 40% in 82 cases (74. 5%). There were 84 cases (76. 5%) with forward motion force (PRA) less than 32%. There were 3 cases (2.73%) of 60min with liquefaction time and 3 cases (2. 7%) with abnormal sperm 96%. There were 274 protein spots in patients with idiopathic azoospermia, which were different from those in the healthy group (the reference level was more than three times as significant as that in the control group), and the difference was statistically significant (P 0.05. 3) among 274 different proteins. A total of 109 protein spots were found in the seminal plasma of idiopathic asthenospermia patients, and the difference was statistically significant (P 0.05). Among them, 99 protein spots were not expressed in the healthy group, and there were 274 different proteins in the seminal plasma of the patients with idiopathic asthenospermia. A total of 165 protein spots were found in the seminal plasma of patients with idiopathic asthenospermia. Among them, 144 protein spots were not expressed in the seminal plasma of idiopathic asthenospermia patients. Above, we excluded smaller protein fragments and unidentified proteins, including heat shock protein, keratin, heat shock protein and keratin. The epididymal secretory protein Ras, histone and prostate stem cell antigen are of significance. It is discussed that they may affect the pathogenesis and development of asthenospermia through different mechanisms. [conclusion] among the abnormal indexes of semen routine, sperm motility is more common in patients with asthenospermia in Kunming area, and the abnormal sperm motility is often accompanied by abnormal sperm count or sperm concentration. A variety of proteins in seminal plasma are involved in the pathogenesis and development of asthenospermia, which have certain significance in judging the severity, prognosis and curative effect of the disease, and can make a preliminary hypothesis. Various proteins play different roles in the occurrence and development of asthenospermia, which lays a foundation for further research.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R698.2

【参考文献】