长链非编码RNA-UBC1对膀胱癌细胞生物学功能的影响

发布时间：2018-05-31 14:24

本文选题：RNA + 膀胱肿瘤　；参考：《重庆医学》2017年02期

【摘要】：目的探讨长链非编码RNA(lncRNA)-尿路上皮癌相关基因1(UBC1)对膀胱癌细胞增殖、凋亡和侵袭转移的作用及其可能的机制。方法采用siRNA干扰技术沉默膀胱癌细胞系T24细胞的lncRNA-UBC1,设置阴性对照组,CCK-8检测细胞增殖,流式细胞仪检测细胞凋亡,Transwell检测细胞侵袭能力,Western blot印迹法检测与转移相关的基质金属蛋白2(MMP-2)与zeste基因增强子同源2(EZH2)。结果 lncRNA-UBC1在T24细胞株中表达明显高于人胚胎膀胱组织来源细胞;与阴性对照组和空白对照组相比,沉默UBC1后,UBC1沉默组膀胱癌细胞增殖速率慢于对照组,UBC1沉默组膀胱癌细胞凋亡细胞比例高于阴性对照组[(18.72±7.79)%vs.(13.09±5.66)%,P0.05],UBC1沉默组细胞侵袭能力受到抑制,T24细胞MMP-2与EZH2表达降低。结论沉默lncRNA-UBC1可抑制T24细胞增殖、凋亡及侵袭能力,其机制可能是通过MMP-2与EZH2途径。
[Abstract]:Objective to investigate the effect of long chain noncoding RNA-lncRNA-UBC1 on the proliferation, apoptosis, invasion and metastasis of bladder cancer cells and its possible mechanism. Methods IncRNA-UBC1 of bladder cancer cell line T24 was silenced by siRNA interference technique, and CCK-8 was used to detect cell proliferation in negative control group. Cell apoptosis was detected by flow cytometry and the invasion ability was detected by Transwell. Western blot was used to detect the matrix metalloprotein 2 (MMP-2) associated with metastasis and zeste gene enhancer 2EZH2. Results the expression of lncRNA-UBC1 in T24 cell line was significantly higher than that in human embryonic bladder tissue derived cells, and was significantly higher than that in negative control group and blank control group. After silencing UBC1, the proliferation rate of bladder cancer cells in UBC1 silencing group was slower than that in UBC1 control group, and the percentage of apoptotic cells in UBC1 silencing group was higher than that in negative control group [18.72 卤7.79)%vs.(13.09 卤5.66p0.05] the cell invasion ability of UBC1 silencing group was inhibited and the expression of MMP-2 and EZH2 in UBC1 silencing group was lower than that in UBC1 silencing group. Conclusion silencing lncRNA-UBC1 can inhibit the proliferation, apoptosis and invasion of T24 cells, and its mechanism may be through MMP-2 and EZH2 pathway.
【作者单位】：湖北省武汉市中心医院泌尿外科;
【分类号】：R737.14

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