FSH对特发性少、弱精子症DNA碎片修复机理初步研究

发布时间：2018-05-31 16:13

本文选题：FSH + 特发性少弱精子症　；参考：《山东中医药大学》2014年硕士论文

【摘要】：目的：初步探讨纯尿促卵泡素（FSH）对特发性少、弱精子症患者DNA碎片的修复机理。通过分析比较注射FSH治疗前后精子浓度、活力、畸形精子率、DNA碎片率及MDA水平，论证FSH能够改善男性生育能力，初步揭示FSH对特发性少、弱精子症患者DNA碎片的修复机理。方法：初步筛选2012年11月—2013年8月在我中心就诊，并符合特发性少、弱精子症西医诊断标准的不育患者，入组病人148例，，规范化采集其精液标本，检测其精液量、精子浓度、活力、畸形精子率以及MDA水平。随机分为三组，分别为生精散组50例、FSH组49例、安慰剂组49例。三组患者均不采取生活干预。生精散组服用生精散（2.3g po tid），用药3个月。FSH组肌肉注射FSH（75IU imqod），用药20天。安慰剂组服用安慰剂（2.3g po tid），用药3个月。通过比较各组每个月治疗前后患者精液的诸项指标，特别是DNA碎片率，初步探讨FSH的作用机理。结果：1、各组临床总体疗效治疗前后的对比：FSH组和生精散组总体疗效优于安慰剂组，有统计学意义（P＜0.05）。两组之间总体疗效比较无统计学意义（P＞0.05）。2、治疗前后精子浓度的对比：生精散组和FSH组治疗后的精子浓度都显著提高，有统计学意义（P＜0.05），且较安慰剂组治疗比较有统计学意义（P＜0.05）。治疗后，FSH组的精子浓度显著高于生精散组，有统计学意义（P＜0.05）。3、治疗前后精子活力的对比：FSH组和生精散组治疗后的前向运动精子和非前向运动精子有显著提高，有统计学意义（P＜0.05），且较安慰剂组治疗比较有统计学意义（P＜0.05），但FSH组和生精散组之间比较无统计学意义（P＞0.05）。4、治疗前后精子畸形率的比较：FSH组治疗后的畸形精子率显著降低，有统计学意义（P＜0.05），且较生精散组和安慰剂组治疗后比较有统计学意义（P＜0.05）。生精散组和安慰剂组治疗后比较无统计学意义（P＞0.05）。5、治疗前后精子DNA碎片率的比较：FSH组和生精散组治疗后的精子DNA碎片率有显著降低，有统计学意义（P＜0.05），且较安慰剂组比较有统计学意义（P＜0.05），但FSH组和生精散组之间比较无统计学意义（P＞0.05）。6、治疗前后精浆MDA水平的比较：FSH组和生精散组治疗后MDA水平有显著降低，有统计学意义（P＜0.05），且较安慰剂组比较有统计学意义（P＜0.05），但FSH组和生精散组之间比较无统计学意义（P＞0.05）。结论：本研究表明精子活力与DNA碎片率具有相关性。FSH通过降低MDA水平，提高精子活力，降低精子DNA碎片率。能够明显改善特发性少、弱精子症患者精液质量（包括提高精子浓度，提高前向运动精子和非前向运动精子活力，降低精子畸形率等），进而提高其生育能力。MDA水平与精子DNA碎片率呈正相关。
[Abstract]:Aim: to investigate the repair mechanism of DNA fragments in patients with idiopathic oligozoospermia by pure urinary follicle stimulating hormone (FSH). By analyzing and comparing sperm concentration, motility, abnormal sperm rate and MDA level before and after FSH injection, it was demonstrated that FSH could improve male fertility, and the repair mechanism of DNA fragments in patients with idiopathic and weak spermatospermia was preliminarily revealed by FSH. Methods: a total of 148 infertile patients who met the diagnostic criteria of idiopathic oligozoospermia and western medicine were preliminarily selected from November 2012 to August 2013. The semen samples were collected from 148 patients, and their semen volume and sperm concentration were measured. Motility, abnormal sperm rate and MDA level. They were randomly divided into three groups: 50 cases of FSH group and 49 cases of placebo group. Three groups of patients did not take life intervention. Shengjingsan group was given 2. 3 g of Shengjingsan po tidai, and the group of 3 months. FSH group was intramuscularly injected with FSH(75IU imqodus for 20 days. The placebo group received placebo 2.3 g po tidd for 3 months. The action mechanism of FSH was preliminarily discussed by comparing the indexes of semen, especially the rate of DNA fragments, before and after every month of treatment in each group. Results compared with the control group before and after treatment, the total curative effect of the two groups was better than that of the placebo group (P < 0.05). There was no significant difference in the total therapeutic effect between the two groups (P > 0.05). The sperm concentration in Shengjingsan group and FSH group was significantly increased after treatment (P < 0.05), and was significantly higher than that in placebo group (P < 0.05). After treatment, the sperm concentration in FSH group was significantly higher than that in Shengjingsan group (P < 0.05). The sperm motility of FSH group was significantly higher than that of non-forward motile sperm group after treatment. There was significant difference (P < 0.05) between FSH group and Shengjingsan group (P < 0.05), but there was no significant difference between FSH group and Shengjingsan group (P > 0.05). There was significant difference (P < 0.05) between Shengjingsan group and placebo group (P < 0.05). There was no significant difference after treatment between Shengjingsan group and placebo group (P > 0.05). The rate of sperm DNA fragment was significantly decreased in the two groups after treatment. There was significant difference (P < 0.05) between FSH group and Shengjingsan group (P < 0.05), but there was no significant difference between FSH group and Shengjingsan group (P > 0.05). The level of MDA in seminal plasma was significantly lower in the control group than that in the control group before and after treatment, and the level of MDA in the control group was significantly lower than that in the control group (P < 0.05). There was significant difference between FSH group and Shengjingsan group (P < 0.05), but there was no significant difference between FSH group and Shengjing San group (P > 0.05). Conclusion: this study indicates that sperm motility is correlated with DNA fragment rate. FSH can decrease MDA level, increase sperm motility and decrease DNA fragment rate. Can significantly improve the semen quality of patients with idiopathic oligozoospermia (including increased sperm concentration, forward motile sperm and non-forward motile sperm motility). There was a positive correlation between the sperm deformity rate and the rate of sperm DNA fragments.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R698

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