血栓素A2受体拮抗剂雷马曲班对足细胞凋亡和nephrin表达的影响

发布时间：2018-08-24 18:19

【摘要】：目的观察血栓素A2(TXA2)受体拮抗剂雷马曲班对嘌呤霉素氨基核苷(PAN)诱导的足细胞凋亡、nephrin mRNA和蛋白表达的影响。方法以条件永生性小鼠足细胞株为研究对象,随机分为4组:对照组、PAN组、雷马曲班组、PAN+雷马曲班组。PAN组加入100μg/mL PAN、雷马曲班组加入30nmol/L雷马曲班、PAN+雷马曲班组加入100μg/mL PAN和30nmol/L雷马曲班,3组均刺激48h。采用流式细胞仪检测足细胞凋亡水平,实时荧光定量RT-PCR检测nephrin mRNA的相对表达量,Western印迹法检测nephrin蛋白的表达状况。结果 PAN组的足细胞凋亡率显著高于对照组、雷马曲班组和PAN+雷马曲班组(P值均0.05),nephrin mRNA和蛋白表达量显著低于对照组、雷马曲班组和PAN+雷马曲班组(P值均0.05)。结论 TXA2受体拮抗剂雷马曲班可以抑制PAN诱导下的足细胞凋亡,使nephrin表达量上调。
[Abstract]:Aim to observe the effect of Ramatoban, a thromboxane A 2 (TXA2) receptor antagonist, on the expression of nephrin mRNA and protein in podocytes induced by purine mycin aminonucleoside (PAN). Methods A conditioned immortalized mouse podocyte cell line was randomly divided into four groups: control group, pan group, and control group. The rats in the three groups were stimulated for 48 hours after adding 100 渭 g/mL PAN, Reimaqu ban to 30nmol/L, 100 渭 g/mL PAN and 100 渭 g/mL PAN, respectively. The apoptosis level of podocyte was detected by flow cytometry, the relative expression of nephrin mRNA was detected by real-time fluorescence quantitative RT-PCR and the expression of nephrin protein was detected by Western blotting. Results the apoptotic rate of podocytes in PAN group was significantly higher than that in control group. The expression of nephrin mRNA and protein was significantly lower in Ramatreban group and PAN rematroban group than in control group. Conclusion Ramatoban, a TXA2 receptor antagonist, can inhibit the apoptosis of podocyte induced by PAN and up-regulate the expression of nephrin.
【作者单位】：江苏大学临床医学院;常熟市第二人民医院肾内科;上海交通大学医学院附属苏州九龙医院肾内科;
【基金】：江苏省苏州市科技计划资助项目(SYSD2011070、SS0716)
【分类号】：R692

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