LIGHT信号通路在鼠衣原体生殖道感染中的作用

发布时间：2018-11-08 15:49

【摘要】：【目的】初步探讨与单纯疱疹病毒糖蛋白D竞争结合疱疹病毒侵入介体的淋巴毒素类似物(lymphotoxin-like inducible protein that competes with glycoprotein D for herpesvirus entry on T cells,LIGHT)在抗衣原体感染免疫及介导衣原体生殖道病理损伤过程的作用。【方法】用1×104IFUs的Mo Pn经生殖道感染野生型(wild type,wt)、LIGHT KO小鼠,每组一半小鼠于感染后49d,再次感染相同剂量的Mo Pn。每隔3-4 d取生殖道分泌物,测定其中衣原体包涵体的数量。初次感染后80d,处死小鼠,眼眶取血,分离血清,用间接免疫荧光法测定其中抗体类型及效价;同时分离生殖道,肉眼观察其输卵管、子宫角水肿程度,然后甲醛固定、切片,HE染色后,显微镜下观察各组织炎性浸润程度和管腔水肿程度。分离小鼠脾细胞,体外用衣原体EB刺激,测定上清中IL-4、IL-5、IL-17和IFN-γ等细胞因子水平。【结果】LIGHT KO小鼠阴道带菌时间与wt组相当,大部分小鼠均在原发感染后28d左右完全清除感染,且均产生对再次感染的免疫力。LIGHT KO和wt小鼠子宫角和输卵管均出现一定程度的病变,但差异无统计学意义。两组小鼠在原发和继发感染Mo Pn后,均产生高效价的特异性抗Mo Pn Ig G抗体,总抗体及各Ig G抗体亚类效价差异均无统计学意义(P0.05),且Ig G2a/Ig G1比值均大于1。和wt小鼠一样,LIGHT KO小鼠脾淋巴细胞经衣原体再次刺激后均可产生较高水平的IFN-γ和IL-17,且未能检测到IL-4和IL-5。【结论】小鼠抗Mo Pn生殖道感染及Mo Pn引起的生殖道病理损伤不依赖于LIGHT信号通路。
[Abstract]:[objective] to explore the lymphotoxin analogues (lymphotoxin-like inducible protein that competes with glycoprotein D for herpesvirus entry on T cells,) that compete with herpes simplex virus glycoprotein D (HSV glycoprotein D) and invade herpes virus (HSV) vectors. [methods] Wild type (wild type,wt), LIGHT KO) mice were infected with 1 脳 104IFUs Mo Pn through the reproductive tract, half of the mice in each group were infected with chlamydia infection on the 49d after infection, [methods] the effect of LIGHT on the immunity against chlamydia infection and the process of chlamydia genital tract pathological injury mediated by 1 脳 104IFUs Mo Pn were observed. Reinfection with the same dose of Mo Pn. Genital secretions were collected every 3-4 days to determine the number of chlamydia inclusions. 80 days after the first infection, the mice were killed, the blood was taken from the orbit, the serum was isolated, and the antibody types and titers were determined by indirect immunofluorescence method. At the same time, the genital tract was separated, the oviduct and uterine horn edema were observed with the naked eye, then fixed with formaldehyde, sectioned and stained with HE, the degree of inflammatory infiltration and the degree of edema in the lumen were observed under microscope. Spleen cells of mice were isolated and stimulated by chlamydia EB in vitro. The levels of cytokines such as IL-4,IL-5,IL-17 and IFN- 纬 in the supernatant were measured. [results] the time of vaginal inoculation in LIGHT KO mice was the same as that in wt group. Most of the mice completely cleared the infection about 28 days after the primary infection, and all the mice developed immunity to the reinfection. The uterine horn and fallopian tube of. LIGHT KO and wt mice showed pathological changes to some extent, but the difference was not statistically significant. After primary and secondary Mo Pn infection, two groups of mice produced high titer specific anti Mo Pn Ig G antibodies. There was no significant difference in the titers of total antibodies and Ig G antibody subclasses (P0.05), and the Ig G2a/Ig G1 ratio was higher than 1. Like wt mice, spleen lymphocytes of, LIGHT KO mice were stimulated by chlamydia to produce higher levels of IFN- 纬 and IL-17,. IL-4 and IL-5. [conclusion] the anti Mo Pn reproductive tract infection and the pathological injury induced by Mo Pn were not dependent on LIGHT signaling pathway in mice.
【作者单位】：南华大学病原生物学研究所;南华大学公共卫生学院;第三军医大学免疫学研究所;
【基金】：国家自然科学基金项目(81001318,30971394) 湖南省教育厅科学研究青年项目(13B099,12B109) 湖南省自然科学基金(13JJ4072) 南华大学博士启动基金(2014XQD42) 特殊病原体防控湖南省重点实验室【湘科计字(2014)5号+湘教通(2012)312号】~~
【分类号】：R691.3

【参考文献】