内源性物质3-硫酸吲哚酚用于肾损伤早期诊断的探讨

发布时间：2019-04-01 08:55

【摘要】：目的研究内源性物质3-硫酸吲哚酚用于肾损伤早期诊断的意义。方法以抗癌药顺铂、甲氨蝶呤为研究对象,取雄性Sprague-Dawley大鼠36只,随机分为3组,分别为顺铂组、甲氨蝶呤组和空白组。于给药后第1、3和5天采集血清与新鲜肾组织。采用LC-MS法测定各组大鼠血清中3-硫酸吲哚酚的质量浓度;同时分析各组大鼠血清中生化指标肌酐和尿素氮浓度以及肾脏组织病理学变化。结果单次给药后,肾组织病理学结果显示顺铂组与甲氨蝶呤组大鼠在第3天均可见明显肾损伤;各组中内源性物质3-硫酸吲哚酚于第3天明显升高;顺铂组肌酐与尿素氮于第5天明显升高,甲氨蝶呤组肌酐与尿素氮无明显变化。结论在顺铂和甲氨蝶呤致肾损伤模型中,内源性物质3-硫酸吲哚酚显示了较常规生化指标血肌酐与尿素氮更高的灵敏度。
[Abstract]:Objective to study the value of endogenous substance 3-indophenol sulfate in early diagnosis of renal injury. Methods 36 male Sprague-Dawley rats were randomly divided into three groups: cisplatin group, methotrexate group and blank group. Serum and fresh renal tissue were collected on the 1st, 3rd and 5th day after administration. The concentration of indolephenol 3-sulfate in serum of rats in each group was determined by LC-MS and the biochemical indexes creatinine and urea nitrogen in serum and histopathological changes of kidney in each group were analyzed at the same time. Results after single administration, renal histopathology showed that both cisplatin group and methotrexate group had obvious renal injury on the 3rd day, the endogenous substance 3-indophenol sulfate significantly increased on the 3rd day, and the renal injury was observed on the 3rd day after the administration of cisplatin and methotrexate. Creatinine and urea nitrogen increased significantly in cisplatin group on the 5th day, but there was no significant change in methotrexate group. Conclusion in the model of renal injury induced by cisplatin and methotrexate, the endogenous substance 3-indophenol sulfate shows a higher sensitivity than routine biochemical markers such as serum creatinine and urea nitrogen.
【作者单位】：沈阳药科大学药学院;亳州职业技术学院;
【基金】：国家自然科学基金资助项目(81373367)
【分类号】：R692

【相似文献】