薯蓣多糖体外对精子存活率和DNA完整性的影响

发布时间：2019-04-04 11:48

【摘要】：目的:通过观察薯蓣多糖体外对精子膜及DNA完整性的影响。为少弱精子症的治疗提供新的途径。方法:正常已生育成年男性,禁欲3~5 d手淫法取精液。分为6组,每组各取0.2 ml精液;对照组为精液原液,其他5组分别加入等量的生理盐水、维生素C溶液及不同浓度薯蓣多糖溶液(0.25、1.0、5.0 mg/ml)。通过伊红-Y染色试验、精子低渗肿胀实验(HOS)以及精子染色质扩散(SCD)实验,观察不同浓度薯蓣多糖体外对精子存活率、精子膜完整性以及精子核DNA的影响。结果:6份精液样本孵育24 h及48 h后各组的精子存活率均有降低。维生素C组精子存活率分别为(28.5±3.1)%、(6.5±1.2)%;薯蓣多糖低、中、高剂量组的精子存活率分别为(31.3±3.5)%、(6.5±2.2)%,(37.1±3.5)%、(9.5±2.8)%,(38.3±3.3)%、(9.0±3.2)%,均显著高于生理盐水组[(13.4±4.1)%、(3.1±2.0)%,P0.01],中、高剂量薯蓣多糖组显著高于维生素C组(P0.05及P0.01)。精子DNA碎片率低剂量薯蓣多糖组为(29.4±2.6)%,与维生素C组[(30.5±2.5)%]无明显差异,但显著低于对照组[(41.7±2.2)%]和生理盐水组[(42.1±3.3)%];中、高剂量薯蓣多糖组精子DNA碎片率分别为(28.5±2.3)%和(27.9±1.9)%,显著低于对照组、生理盐水组及维生素C组(P0.05及P0.01)。结论:薯蓣多糖具有体外提高精子存活率和保护精子DNA完整性的作用。
[Abstract]:Objective: to observe the effect of Dioscorea zingiberensis polysaccharide on sperm membrane and DNA integrity in vitro. It provides a new way for the treatment of oligoasthenospermia. Methods: normal fertile adult male, abstinence 3 days 5 days by hand to get semen. Six groups were divided into 6 groups, each group received 0.2 ml semen, the control group was semen fluid, the other 5 groups were added the same amount of saline, vitamin C solution and different concentration of dioscorea solution (0.25, 1.0, 5.0 mg/ml). The effects of Dioscorea zingiberensis on sperm viability, sperm membrane integrity and sperm nuclear DNA were observed in vitro by eosin-Y staining, sperm hypoosmotic swelling test (HOS) and sperm chromatin diffusion (SCD) test. Results: the sperm survival rate of each group decreased after 24 h and 48 h incubation with 6 semen samples. The sperm survival rate in vitamin C group was (28.5 卤3.1)% and (6.5 卤1.2)%, respectively. The sperm survival rates in the high dose group were (31.3 卤3.5)%, (6.5 卤2.2)%, (37.1 卤3.5)%, (9.5 卤2.8)%, (38.3 卤3.3)%, (9.0 卤3.2)%, respectively. It was significantly higher than that in saline group [(13.4 卤4.1)%, (3.1 卤2.0)%, P0.01], and that in high-dose dioscorea group was significantly higher than that in vitamin C group (P0.05 and P0.01). The sperm DNA fragmentation rate was (29.4 卤2.6)% in the low-dose dioscorea group, which was not significantly different from that in the vitamin C group [(30.5 卤2.5)%]. But it was significantly lower than that of the control group [(41.7 卤2.2)%] and the saline group [(42.1 卤3.3)%]. The DNA fragmentation rate of high dose dioscorea polysaccharide group was (28.5 卤2.3)% and (27.9 卤1.9)% respectively, which was significantly lower than that of control group, physiological saline group and vitamin C group (P0.05 and P0.01). Conclusion: Dioscorea zingiberensis can improve sperm survival rate and protect sperm DNA integrity in vitro.
【作者单位】：山东省妇幼保健院遗传中心国家卫生和计划生育委员会生育调控技术重点实验室;
【基金】：山东省科技发展计划(2015GSF118072)~~
【分类号】：R698.2

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