脂多糖经TLR4介导Cx43的表达对膀胱癌细胞增殖和凋亡的影响

发布时间：2019-07-24 21:00

【摘要】：目的:探讨脂多糖对膀胱癌细胞株Cx43和TLR4蛋白的表达,及其对细胞增殖和凋亡的影响。方法:体外培养膀胱癌5637细胞,在LPS刺激下,采用蛋白质印迹法检测膀胱癌5637细胞中TLR4和Cx43蛋白的表达,通过CCK-8检测5637-control组和5637-LPS组细胞的增殖情况;利用流式细胞术检测5637-control组、5637-LPS组和5637-LPS+顺铂组细胞的凋亡情况。结果:在LPS刺激下,膀胱癌5637细胞中的Cx43基因表达较对照组明显增高,两组之间比较差异有统计学意义(P=0.012),而5637-LPS组(LPS,2μg/mL,24 h)中TLR4及Cx43的表达均较5637-control组明显增高,差异有统计学意义(P=0.019,P=0.003)。CCK-8结果显示,在LPS刺激下,5637-LPS组随着时间(0、1、2、3 d)的增加,细胞的增殖较5637-control组明显,差异有统计学意义(P=0.018)。流式细胞仪检测结果显示,5637-control组和5637-LPS组凋亡率分别为(8.3±1.58)%和(7.8±2.03)%,差异无统计学意义(P=0.099)。而5637-顺铂组凋亡率[(60±4.35)%]较5637-顺铂+LPS组[(52±6.25)%]高,差异有统计学意义(P=0.019)。结论:在LPS刺激下,膀胱肿瘤细胞可能通过提高TLR4和Cx43的表达来介导肿瘤细胞逃脱免疫监视。
[Abstract]:Aim: to investigate the expression of lipopolysaccharide (lipopolysaccharide) on bladder cancer cell line Cx43 and TLR4, and the effect of lipopolysaccharide on cell proliferation and apoptosis. Methods: bladder cancer cell line 5637 was cultured in vitro. The expression of TLR4 and Cx43 protein in bladder cancer 5637 cell line was detected by Western imprinting, the proliferation of 5637-control group and 5637-LPS group was detected by CCK-8, and the apoptosis of 5637-control group, 5637-LPS group and 5637-LPS platinum group was detected by flow cytometry. Results: the expression of Cx43 gene in bladder cancer 5637 cells stimulated by LPS was significantly higher than that in the control group (P 鈮，

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