类风湿关节炎患者基质金属蛋白酶-3的检测

发布时间：2017-12-28 21:17

本文关键词：类风湿关节炎患者基质金属蛋白酶-3的检测　出处：《广东医学》2016年04期 　论文类型：期刊论文

【摘要】：目的探讨类风湿性关节炎(rheumatoid arthritis,RA)患者血清基质金属蛋白酶-3(MMP-3)的水平变化及意义。方法收集RA患者90例,其中活动期45例(活动期组),缓解期45例(缓解期组),另收集健康体检者45例为健康对照组。采用流式液相蛋白定量技术(CBA)检测RA患者血清IL-17、IL-18、IL-6的水平,采用酶联免疫法(ELISA)检测MMP-3的水平。结果活动期组、缓解期组MMP-3、IL-17、IL-18、IL-6的水平显著高于健康对照组(P0.01);活动期组MMP-3、IL-17、IL-18、IL-6的水平显著高于缓解期组(P0.01);活动期RA患者MMP-3与抗CCP抗体、RF、CRP、ESR、IL-17、IL-18、DAS58评分、关节压痛数、关节肿胀数呈正相关。结论 MMP-3与RA病理性炎症损伤及疾病的进展有关,MMP-3与IL-17、IL-18、IL-6联合检测可作为RA早期诊断及疗效判断的依据。
[Abstract]:Objective to explore the changes and significance of serum matrix metalloproteinase -3 (MMP-3) in patients with rheumatoid arthritis (RA). Methods 90 patients with RA were collected, of which 45 were active (active stage), 45 remission period (remission stage), and 45 healthy subjects as healthy control group. The level of IL-17, IL-18 and IL-6 in serum of RA patients was detected by flow liquid protein quantitative technique (CBA), and the level of MMP-3 was detected by enzyme linked immunoassay (ELISA). The active group and remission group MMP-3, IL-17, IL-18, IL-6 levels were significantly higher than the healthy control group (P0.01); group MMP-3, IL-17 activity and IL-18, IL-6 level was significantly higher than the remission group (P0.01); activities related to RA patients with anti CCP antibody, MMP-3, CRP, ESR, RF IL-17, IL-18, DAS58 score, joint tenderness, joint swelling number of positive. Conclusion MMP-3 is associated with the development of RA's pathological inflammation and disease. The combined detection of MMP-3 and IL-17, IL-18 and IL-6 can be used as a basis for early diagnosis and curative effect judgement of RA.
【作者单位】：承德医学院附属医院检验科;
【基金】：河北省承德市科学技术研究与发展计划项目(编号:20132059)
【分类号】：R593.22;R446.11
【正文快照】： 类风湿关节炎(RA)是一种以慢性、对称性、关节滑膜炎症为主要临床表现的系统性自身免疫性疾病。主要病理表现为滑膜细胞增生、炎症细胞浸润、软骨基质降解,血管翳形成[1],严重影响着患者的健康及生活质量。RA的发病机制至今尚未明确[2],因此,寻找早期能判断RA患者病情进展的指

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