沉默长链非编码RNA Meg3损伤小鼠胰岛细胞的胰岛素分泌功能

发布时间：2017-12-31 20:08

本文关键词：沉默长链非编码RNA Meg3损伤小鼠胰岛细胞的胰岛素分泌功能　出处：《中国生物化学与分子生物学报》2016年03期 　论文类型：期刊论文

【摘要】：MEG3是一种长链非编码RNA。已有研究证明,鼠源Meg3参与小鼠诱导多能干细胞、神经元和视网膜的分化过程。最新报道,MEG3在人胰岛β细胞中高表达,但其对维持成年胰岛β细胞的功能尚不清楚。本研究旨在探讨Meg3在小鼠胰岛细胞胰岛素分泌功能中的作用。实时定量PCR揭示,与Balb/c小鼠心、肝、脾、肺、肌、肾等组织/器官比较,Meg3在胰腺组织中高表达。在非糖尿病小鼠发生自发性糖尿病的第8、12周,Meg3在胰岛中的表达水平分别下调24%±8%和29%±9%(P0.01);而当血糖升高20 mmol/L,小鼠胰岛中Meg3表达下调72%±16%(P0.01)。在MIN6细胞中采用RNA干扰敲减Meg3的表达,在高糖浓度(20 mmol/L)刺激条件下,胰岛素分泌显著减少。小鼠静脉注射si RNA,结合血糖测定或葡萄糖耐受试验(IPGTT)显示,si-Meg3小鼠血清胰岛素水平显著下降。注射葡萄糖前血糖升高,注射葡萄糖后耐受能力降低;免疫组化分析显示,si-Meg3小鼠胰岛素阳性细胞的面积减少。实验结果提示,Meg3通过参与胰岛素的合成和分泌维持成年小鼠胰岛功能。Meg3表达失调可能参与I型糖尿病(T1DM)发病过程。
[Abstract]:MEG3 is a long chain of non existing research on RNA. encoding murine Meg3 in mice demonstrated that induced pluripotent stem cells, differentiation of neurons and retina. The latest reports, the high expression of MEG3 in human pancreatic beta cells, but the maintenance of adult islet beta cell function is unclear. This study aims to explore the Meg3 the role of insulin secretion in mouse islet cells. Real time quantitative PCR revealed, and Balb/c mice heart, liver, spleen, lung, muscle, kidney tissue / organ, the high expression of Meg3 in pancreatic tissue. The spontaneous diabetes occurred in non diabetic mice at 8,12 weeks, the expression level of Meg3 in islets respectively. By 24% + 8% and 29% + 9% (P0.01); and when the blood glucose increased 20 mmol/L, down 72% + 16% Meg3 expression in mouse islet beta (P0.01). The expression by RNA interference in MIN6 cells Meg3 knockdown, in high glucose concentration (20 mmol/L) stimulation conditions, insulin secretion significantly Reduce. Mice were injected with Si RNA, combined with the determination of blood sugar or glucose tolerance test (IPGTT) showed that the serum insulin level were significantly decreased. Si-Meg3 before the injection of glucose tolerance to reduce hyperglycemia after glucose injection; immunohistochemical analysis showed that si-Meg3 mouse insulin positive cells decreased. The results suggest that the Meg3 involved in the synthesis and secretion of insulin to maintain adult mouse islet function.Meg3 expression imbalance may participate in type I diabetes mellitus (T1DM) patients.

【作者单位】：铜陵职业技术学院医学系;南京医科大学基础医学院;
【基金】：安徽省高校自然科学研究基金重点项目(No.KJ2015A374)资助~~
【分类号】：R587.1
【正文快照】： 安徽省高校自然科学研究基金重点项目(No.KJ2015A374)资助*联系人E-mail:Tel:0562-2802925;20534361@qq.comReceived:October 6,2015;Accepted:January 7,2016Supported by Colleges and Universities Natural Science Foundation of Anhui Province(No.KJ2015A374)*Correspon

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