SLE患者外周血T淋巴细胞p53基因表达及其与DNA甲基化的相关性

发布时间：2018-01-06 14:22

本文关键词：SLE患者外周血T淋巴细胞p53基因表达及其与DNA甲基化的相关性　出处：《中国皮肤性病学杂志》2016年04期 　论文类型：期刊论文

【摘要】：目的观察系统性红斑狼疮(SLE)患者外周血T淋巴细胞p53基因表达情况及探讨其与DNA甲基化的相关性。方法收集34例SLE患者与23例健康对照者,均取外周血T淋巴细胞,分别用5-甲基胞嘧啶抗体与流式细胞仪检测DNA甲基化状态和反转录-聚合酶链反应(real time RT-PCR)分析p53mRNA表达情况。结果 SLE患者DNA甲基化水平活动期(8.50±1.42)与正常人(11.31±1.34)相比,差异有统计学意义(P0.01)。SLE患者p53基因表达活动期(1.15±0.33)与正常人(0.81±0.27)相比,差异也有统计学意义(P0.01)。SLE患者p53基因的表达与狼疮疾病活动指数(SLEDAI)正相关(P0.01);而与DNA甲基化水平没有明显的相关性(P0.05)。结论 p53基因在外周血T淋巴细胞中的异常表达与SLE的发病相关,p53基因的异常表达在SLE表观遗传学发病机制的作用有待进一步研究。
[Abstract]:Objective To observe the effect of systemic lupus erythematosus (SLE) of T p53 lymphocytes in peripheral blood of patients with gene expression and investigate the correlation between DNA methylation. Methods 34 cases of SLE patients and 23 healthy controls were taken from peripheral blood T lymphocytes were used to detect the methylation status of DNA 5- methylcytocine antibody and flow cytometry cytometry and reverse transcription polymerase chain reaction (real time RT-PCR) to analyze the expression of p53mRNA. Results the level of activity of DNA in patients with SLE methylation (8.50 + 1.42) period and normal people (11.31 + 1.34) compared to the difference was statistically significant (P0.01) in patients with.SLE p53 gene expression in active stage (1.15 + 0.33) and normal people (0.81 + 0.27) compared to the difference was statistically significant (P0.01) in patients with.SLE p53 gene expression and disease activity index of SLE (SLEDAI) positive correlation (P0.01); while there was no obvious correlation with the methylation level of DNA (P0.05). Conclusion p53 gene in peripheral blood T Abnormal expression in lymphocytes is associated with the pathogenesis of SLE, and the role of abnormal expression of p53 gene in the pathogenesis of SLE epigenetics remains to be further studied.

【作者单位】：复旦大学附属华山医院皮肤科;
【基金】：国家自然科学基金(81371745) 上海市自然科学基金(12ZR1404500)
【分类号】：R593.241
【正文快照】： 系统性红斑狼疮(SLE)是一种病因未明的累及全身多器官的慢性进行性病谱性自身免疫病。SLE表观遗传学的研究显示,DNA低甲基化为解释SLE发病机制和开发新的治疗策略提供了新的线索。DNA低甲基化通过促进CD11a/CD18,CD70等共刺激分子的表达,首先使T细胞具有自身反应性,活化的T细

【相似文献】