丝胶蛋白对链脲佐菌素诱导胰岛细胞株INS-1凋亡相关因子的影响研究

发布时间：2018-01-27 16:04

  本文关键词： 丝胶蛋白质类 链脲菌素 凋亡诱导因子 基因 Bcl- Bcl-相关X蛋白质　出处：《中国全科医学》2017年14期 　论文类型：期刊论文

【摘要】：目的探讨丝胶蛋白对链脲佐菌素(STZ)诱导胰岛细胞株INS-1凋亡相关因子B淋巴细胞瘤2(Bcl-2)、Bcl-2相关X蛋白(Bax)的影响。方法 2015年6月—2016年3月,将大鼠胰岛细胞株INS-1分为3组:正常组胰岛细胞株INS-1不作任何处理,STZ组以STZ作用胰岛细胞株INS-1,丝胶蛋白组以STZ和丝胶蛋白作用胰岛细胞株INS-1。CCK-8法检测各组细胞存活率;流式细胞术检测各组细胞凋亡率;实时荧光定量聚合酶链式反应(RT-PCR)法和Western blotting法检测凋亡相关因子Bcl-2和Bax mRNA表达及其蛋白的表达。结果 STZ组胰岛细胞株INS-1细胞存活率较正常组降低,丝胶蛋白组胰岛细胞株INS-1细胞存活率较STZ组升高(P0.05)。流式细胞术检测结果显示,STZ组胰岛细胞株INS-1细胞凋亡率较正常组升高,丝胶蛋白组胰岛细胞株INS-1细胞凋亡率较STZ组降低(P0.05)。STZ组胰岛细胞株INS-1 Bcl-2 mRNA及其蛋白表达水平、Bcl-2 mRNA/Bax mRNA、Bcl-2/Bax较正常组降低,Bax mRNA及其蛋白表达水平较正常组升高(P0.05);丝胶蛋白组胰岛细胞株INS-1 Bcl-2 mRNA及其蛋白表达水平、Bcl-2mRNA/Bax mRNA、Bcl-2/Bax较STZ组升高,Bax mRNA及其蛋白表达水平较STZ组降低(P0.05)。结论丝胶蛋白可抑制STZ诱导的胰岛细胞株INS-1的凋亡,提高Bcl-2/Bax的比值。
[Abstract]:Objective to investigate the effect of sericin on streptozotocin (STZ) -induced pancreatic islet cell line INS-1 apoptosis-related factor B lymphocytoma (Bcl-2). The effect of Bcl-2 related X protein Bax.Methods from June 2015 to March 2016. Rat islet cell line INS-1 was divided into three groups: normal islet cell line INS-1 was treated with STZ without any treatment and islet cell line INS-1 was treated with STZ. STZ and sericin treated islet cell line INS-1.CCK-8 were used to detect the survival rate of each group. Apoptosis rate was detected by flow cytometry. Real-time quantitative polymerase chain reaction (RT-PCR). The expression of apoptosis-related factors Bcl-2 and Bax mRNA and its protein expression were detected by Western blotting method. The survival rate of INS-1 cells in STZ group was lower than that in normal group. The survival rate of INS-1 cells in sericin group was higher than that in STZ group (P 0.05). The apoptosis rate of INS-1 cells in STZ group was higher than that in normal group. The apoptotic rate of INS-1 cells in sericin group was lower than that in STZ group. The expression level of INS-1 Bcl-2 mRNA and its protein in islet cell line INS-1 Bcl-2 in sericin group was lower than that in STZ group. Bcl-2 mRNA/Bax mRNAs Bcl-2 / Bax decreased the expression level of Bax mRNA and its protein in Bcl-2 / Bax group compared with the normal group, and the expression level of Bcl-2 / Bax protein was higher than that of the normal group. The expression level of INS-1 Bcl-2mRNA and its protein in sericin group islet cell line was determined by Bcl-2mRNA-Bax mRNA. Bcl-2/Bax was higher than that in STZ group. The expression level of Bax mRNA and its protein was lower than that of STZ group. Conclusion sericin protein can inhibit the apoptosis of islet cell line INS-1 induced by STZ. The ratio of Bcl-2/Bax was increased.
【作者单位】： 承德医学院基础医学研究所;承德医学院附属医院神经外科;承德医学院中药研究所;承德医学院人体解剖学教研室;
【基金】：国家自然科学基金资助项目(81441133) 河北省自然科学基金资助项目(H2013406096) 河北省教育厅青年基金项目(QN20131089)
【分类号】：R587.1
【正文快照】： 有研究者预测,到2030年,全球糖尿病患者人数将增加至5.25亿,其中90%的患者为2型糖尿病[1],2型糖尿病严重影响着人类的健康并且已经成为公共卫生系统的沉重负担[2]。有研究表明,2型糖尿病时细胞凋亡是造成胰岛β细胞功能受损的重要原因,胰岛β细胞凋亡可造成胰岛结构的破坏和功，

本文编号：1468851