水蕨抗艾滋病病毒蛋白CVNH及其突变体在毕赤酵母中的表达（英文）

发布时间：2018-01-29 03:04

本文关键词： 抗HIV蛋白水蕨突变体毕赤酵母　出处：《中山大学学报(自然科学版)》2017年04期 　论文类型：期刊论文

【摘要】：CVN(Cyanovirin-N)是一种高效的抗HIV蛋白。Cyanovirin-N(CVNH)家族是CVN的同源蛋白,具有与CVN的肽链折叠类型并且具有高度保守的抗HIV的结构域。把水蕨Ceratopteris thalictroides野生型和突变型CtCVNH蛋白的编码序列分别克隆到真核表达载体pP ICZαA,结果所有野生型wC tC VNH和突变型mCtCVNH基因分别产生两条带,Western blot确认大小分别为22 000和24 000。采用超滤离心和Ni-NTA亲和色谱法纯化蛋白质,糖基化检测证实wC tC VNH蛋白不存在N-糖基化位点,可能的原因是载体pPICZαA中α因子信号肽序列的不完全切割或存在一些非糖基化的其它翻译后修饰,为今后进一步研究CtCVNH的药代动力学和药效学奠定了坚实的基础。
[Abstract]:CVN Cyanovirin-N) is a highly efficient family of anti HIV protein. Cyanovirin-Ncv CVN family is a homologous protein of CVN. Has a peptide chain folding type with CVN and has a highly conserved domain of resistance to HIV. Ceratopteris. The encoding sequences of thalictroides wild-type and mutant CtCVNH protein were cloned into eukaryotic expression vector pP ICZ 伪 A. Results all wild-type wC tCVNH and mutant mCtCVNH genes produced two bands respectively. The size of Western blot was 22 000 and 24 000, respectively. The protein was purified by ultrafiltration centrifugation and Ni-NTA affinity chromatography. The results of glycosylation showed that there was no N-glycosylation site in WTC VNH protein. This may be due to incomplete cleavage of the 伪 -factor signal peptide sequence in the vector pPICZ 伪 A or other non-glycosylated post-translational modifications. It lays a solid foundation for the further study of pharmacokinetics and pharmacodynamics of CtCVNH.
【作者单位】：中山大学生命科学学院;南京农业大学生命科学学院;华南农业大学生命科学学院;中山大学深圳研究院;
【基金】：国家自然科学基金(31370364,31570652,31670200) 深圳市科技创新委员会资助项目(JCYJ20160425165447211)
【分类号】：Q78;R512.91
【正文快照】： AIDS(Acquired Immune Deficiency Syndrome)of the possible occurance of typical drawbacks such asis a global concern epidemic caused by human immu-short plasma half-life,proteolysis,and immunogenici-nodeficiency virus(HIV),which forces a great impactty,it

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