落新妇苷对高糖刺激的血管内皮细胞SDF-1α表达的影响

发布时间：2018-02-01 13:55

  本文关键词： 高糖 落新妇苷 间质细胞衍生因子α 人脐静脉内皮细胞　出处：《中国病理生理杂志》2016年04期 　论文类型：期刊论文

【摘要】：目的:分析落新妇苷对高糖刺激的血管内皮细胞间质细胞衍生因子1α(SDF-1α)表达的影响。方法:SD大鼠腹腔注射链脲佐菌素后诱导成急性糖尿病模型,采用硫代巴比妥酸法测量血浆丙二醛(MDA)浓度,采用紫外分光光度法检测血浆H2O2浓度,采用免疫荧光检测大鼠胸主动脉内皮细胞中SDF-1α的表达。人脐静脉内皮细胞(HUVECs)经高糖刺激后,用real-time PCR、ELISA和免疫荧光检测SDF-1α的表达,流式细胞术观察细胞周期分布情况,免疫荧光检测p65的表达和细胞内定位。结果:糖尿病大鼠血浆MDA和H2O2水平均高于正常对照组,其胸主动脉内皮细胞SDF-1α的表达较正常组减弱;落新妇苷能降低糖尿病大鼠血浆MDA及H2O2的水平并增加胸主动脉内皮细胞SDF-1α的表达。体外高糖刺激呈浓度和时间依赖性减少HUVECs中SDF-1α表达,落新妇苷能一定程度地恢复SDF-1α表达。高糖刺激增加HUVECs S期比例,而落新妇苷减少其S期分布。高糖刺激HUVECs中p65蛋白核转位,落新妇苷抑制高糖诱导的p65核转位。结论:落新妇苷能增加高糖刺激下内皮细胞表达SDF-1α,减少高糖状态下的氧化应激和NF-κB的活化。
[Abstract]:Objective: to analyze the effect of Acanthrin on vascular endothelial cell mesenchymal cell derived factor-1 伪 (SDF-1 伪) stimulated by high glucose. Methods Acute diabetic model was induced by intraperitoneal injection of streptozotocin into SD rats. Plasma malondialdehyde (MDA) concentration was measured by thiobarbituric acid method and plasma H _ 2O _ 2 concentration was detected by ultraviolet spectrophotometry. The expression of SDF-1 伪 in rat thoracic aortic endothelial cells was detected by immunofluorescence. Human umbilical vein endothelial cells (HUVECs) were stimulated with high glucose and real-time PCR was used. The expression of SDF-1 伪 was detected by ELISA and immunofluorescence, and the distribution of cell cycle was observed by flow cytometry. Results: the levels of MDA and H _ 2O _ 2 in plasma of diabetic rats were higher than those of normal control group. The expression of SDF-1 伪 in the endothelial cells of thoracic aorta was lower than that in the normal group. Acanthrin could decrease the levels of plasma MDA and H2O2 and increase the expression of SDF-1 伪 in thoracic aortic endothelial cells in diabetic rats. In vitro, hyperglycemic stimulation decreased the expression of SDF-1 伪 in HUVECs in a dose-and time-dependent manner. SDF-1 伪 expression. The expression of SDF-1 伪 was restored to some extent by Acanthrin, and the proportion of HUVECs S phase was increased by high glucose stimulation. But Acanthoside reduced its S phase distribution. High glucose stimulated the nuclear translocation of p65 protein in HUVECs. Conclusion: Acanthrin can increase the expression of SDF-1 伪 in endothelial cells stimulated by high glucose, and decrease the oxidative stress and the activation of NF- 魏 B in endothelial cells induced by high glucose.
【作者单位】： 广东医学院病理学系;广东医学院附属医院病理诊断与研究中心;肇庆市第一人民医院儿科;广东医学院基础医学院病理生理教研室;
【基金】：广东省大学生创新训练项目(No.1057112014) 广东医学院大学生创新实验项目(No.2011ZZZF002) 国家自然科学基金资助项目(No.81170121)
【分类号】：R587.1
【正文快照】： 糖尿病是危害人群健康的常见病、多发病。广东省是中国的高发区之一,发病率超过全国平均水平,且年轻化趋势明显[1-2]。糖尿病的一个主要特征就是高血糖症。在长期的高糖刺激下,血管内皮细胞容易受到损伤。而血管内皮细胞内有关信号的活化,进而导致内皮细胞出现分泌细胞因子功，

本文编号：1482016